عليكم السالم

Culturing technique

SwabBroth agar

Flaming loop and needle

Flaming loop :

it is a aseptic technique to assure that contaminates r not introduced into a specimen.

How can we make it

be attention please

Condition of incubation

temperature = it depend on me

ph ( acidophils ,neutrophils

,alkalophils )

Gaseous requirments

How to red the plates

Slide of becteria and (gs):

What is gram staining ?

Is common technique used to differentiate two large group of becteria based in their cell wall .

GRAM +VE : stain violet ( thick layer of peptidoglycan )

GRAM -VE :red stain (thinner peptidoglycan ) which doesn’t retain tha crystal violet during tha decoloring process ..

Any comment …..!

Thank u for listening ..

Hala Kutbi