Cv dr. ravi kumar chilukoti revised_march 2017

1 Flat no 101, Sai Ram Palace, 16th A Cross, Neeladri Road, Electronic City Phase 1, Bangalore- 560100. INDIA

E-mail: [email protected], Phone (Mobile): +91-99590 13223

CURRICULUM VITAE

Dr. Ravi Kumar Chilukoti , PhD [email protected]

Phone: +91-99590 13223 Research Profile: ResearchGate Good team leader, with the capability of working as an individual team player as well, having strong cross-functional domain expertise encompassing:

➢ NGS applications: Clinical exome sequencing, WGS (HiSeqx X10), WES (HiSeq 2500/4000), Amplicon Seq, RNA-Seq (HiSeq 4000), small RNA Seq (HiSeq 2500), Targeted sequencing etc.

➢ Genomics: Transcriptome/miRNA profiling/Chromosomal Microarray analysis using Affymetrix/Agilent arrays, GWAS/SNP genotyping using Affymetrix/SEQUENOM MassARRAY/Sanger sequencing (3730XL/3130)/TaqMan/Exiqon LNA/ Fluidigm

➢ Proteomics: Gel-free/gel-based Protein expression analysis using LC-MSMS/ABI 4800 MALDI TOF-TOF

➢ Cell culture: HL-1, HUVEC, SMC, Primary Cardiac Fibroblasts, HEK 293, L929, 3T3-L1 ➢ Data Analysis tools: Rosetta Resolver, Genedata Analyst, Genedata Expressionist,

Expression Console & Genotyping Console (Affymetrix), Rosetta Elucidator, Proteome Discoverer, Scaffold3, PLINK, Quicktest, SNPTEST, Metal, Haploview, WGAViewer, R (basic script writing knowledge), Copy Caller, Data Assist, Array Assist

➢ Pathway analysis tools: IPA, GenMAPP, Panther ➢ Food-Molecular Biology: Rapid screening and quantification of Genetic Modifications in

Foods, Rapid detection of Food-Borne Pathogens by RT-PCR and Rapid quantification of Allergens in Foods

Specialties: Good at 'Omics' data translation to hypothetical cell signaling cascades. 24.10.2016 to till date: MedGenome Labs Pvt Ltd., Bengaluru, India

Currently working as Senior Scientist (Project Manager) and managing NGS/Microarray based projects/clinical samples that include Clinical Exome Sequencing, WGS, WES, Amplicon Seq, RNA-Seq, small RNA Seq, Targeted sequencing and Chromosomal Microarray Analysis etc.

Job profile includes, but not limited, the following responsibilities in the inter-disciplinary areas:

➢ Oversee and manage all the Indian Academic commercial service projects (NGS and Microarray).

Summary

Current Research Position & Past Experience

mailto:[email protected]


https://www.researchgate.net/profile/Ravi_Kumar_Chilukoti



➢ End to end management starting from sample receipt (coordinating with customer support), QC analysis

➢ Coordination of lab team, sample processing (coordinating with respective lab teams), data generation, QC of samples

➢ reports/data analysis (coordinating with bioinformatics). Proactive follow up of all the processes

➢ Effective communication with all the clients and ensure that project timelines are met ➢ Involves a lot of technical know-how on NGS projects ➢ Helping sales/BD with technical proposals, following SOPs, pricing calculations etc. ➢ Visiting clients when needed and discuss the project progress. ➢ Any other responsibility assigned by COO

28.12.2015 to August 2016: VIMTA Life Sciences, Hyderabad, India

Rejoined VIMTA in December 2015, and worked as Deputy Manager – Genomics & Food-Molecular Biology. As a HOD for these two Departments, managing operations, personnel of Genomics facility and Food safety testing labs. Job profile includes the following responsibilities in the inter-disciplinary areas:

Genomics:

➢ To ensure a smooth process flow - from sample receipt to reporting ➢ To expand and provide high quality services in the areas of

transcriptome/miRNA/genotyping/CNV/Chromosomal Microarray analysis using Affymetrix microarray platform, as an Affymetrix Authorized Service Provider

➢ To analyze and interpret complex 'omics' data, integrated analysis of genomic and other biological and medical data, statistical data analysis, data mining, and database and informatics work

➢ Bio-similar quality assessment using standard molecular biology methods ➢ Project planning, execution, data analysis, interpretation and reporting ➢ To review results and trouble-shoot. ➢ To handle technical complaints or queries. ➢ To ensure timely delivery of reports/results. ➢ To train existing and new joiners, data analysis for customer projects, finding solutions

to customer related queries, troubleshooting, providing technical/scientific guidance to BD/scientific teams as and when required.

Molecular Diagnostics (CRL): ➢ To work in association with Cytogenetics department to provide Chromosomal

Microarray Analysis (CMA) to their existing/new customers ➢ Data analysis and interpretation of CMA results in accordance with phenotype or clinical

case history

Food safety testing (Molecular Testing): ➢ To establish a central and satellite RT-PCR labs for food/GMO/Allergin testing across

India




➢ To implement protocols for food safety testing using standard molecular biology methods

➢ Rapid screening and quantification of Genetic Modifications in Foods, Rapid detection of Food-Borne Pathogens by RT-PCR and Rapid quantification of Allergens in Foods

Personnel Management: ➢ To assign roles and delegate responsibilities to team members. ➢ To plan for training needs and schedules such as food safety testing, Chromosomal

Microarray Analysis etc. ➢ To ensure implementation of quality systems in association Quality Assurance staff

17.06.2015 to 18.12.2015: Imperial Life Sciences (P) Limited, Gurgaon, New Delhi, India

Worked as National Team Lead for Genomics Applications. As a team lead at ILS, lead the Genomics application team and coordinated activities related to Affymetrix, Agena, Nanostring and PacBio. Activities include training sessions, data analysis for customer projects, finding solutions to customer related queries, troubleshooting, providing technical/scientific guidance to sales/research teams as and when required. Furthermore, was responsible for data analysis and reporting of Cytogenetic Microarray Analysis under Clinical Diagnostics division.

01.12.2014 to 31.05.2015: Department of Medical Biochemistry and Molecular Biology (IMBM), Universitätsmedizin Greifswald, Germany.

At IMBM, continued the projects within the EUTRAF related to molecular cardiology/anatomy & cell biology, under the guidance of Prof. Dr. Uwe Lendeckel. Primarily responsible for transcriptome analysis and subsequent in-depth cell signaling analysis in many projects within the EUTRAF (European Network for translational research in atrial fibrillation) consortium, with the objective of studying the etiology of underlying mechanisms in atrial fibrillation (AF).

Based on the transcriptomics data, the impact of the risk factors such as arterial hypertension, heart failure, diabetes mellitus or ageing in contributing the generation and persistence of Atrial Fibrillation is being assessed. To achieve this purpose, various animal (in vivo) and cell/tissue culture (in vitro) models such as Pigs, spontaneously hypertensive rats (SHR), Diet Induced Diabetic (Zucker Diabetic Fatty/ ZDF) Rats, HL1 (Murine Cardiomyocytes), Primary Cardiac Fibroblasts etc. are being used to simulate the AF and other risk factors.

15.08.2008 to 31.12.2014: Department of Functional Genomics, University of Greifswald, Greifswald, Germany.

Worked in the in the Department of Functional Genomics. Area of work related to Molecular Cardiology/Anatomy & Cell Biology. Primarily responsible for transcriptome analysis and subsequent in-depth cell signaling analysis in many projects within the EUTRAF consortium. Determined the impact of the risk factors such as arterial hypertension, heart failure, diabetes




mellitus or ageing in contributing the generation and persistence of Atrial Fibrillation. I served as primary- and co-author in several manuscripts both published in peer-reviewed journals and in the process of being published.

Furthermore, actively involved in studying the effects of dronedarone on gene

regulation in the infarcted area and border zone in a porcine model of anterior myocardial infarction. Also, involved in the Transcriptome/Pharmacogenomics/Protein Expression analysis of the projects from medical departments such as assessing the impact of arterial wall shear stress (HUVEC, HCASMC cell culture models) on atherosclerosis, anatomy (glomerular hypertension/podocytes), physiology (T2DM with impaired glucose tolerance) etc.

Additionally, played a key role in a project aimed to identify potential circulating miRNA

biomarkers of BMI, age and sex in SHIP-Trend cohort. Furthermore, participated in the data analysis of Genome-Wide Association Studies

(GWAS) using the SHIP cohort (cIMT phenotype).

01.04.2007 to 11.08.2008: VIMTA Life Sciences, Hyderabad, India

Worked as a Group Leader-Genomics in VIMTA Life Sciences, Hyderabad, India. Here I am responsible for Affymetrix Microarray platform, SEQUENOM MassARRAY Platform.

Job Profile: Performance and supervision of activities related to Genomics Division especially Affymetrix Microarray platform and SEQUENOM MALDI-TOF platform. It includes the supervision of operations in these two platforms and analysis of results, preparing/reviewing the Standard Operating Procedures (SOP’s), standardizing & validating the SOP’s, train the Scientists and supervision of scientist’s activities in the lab and ensuring that lab activities are carried out in accordance with SOP's etc... And played a key role in getting the authorization for VIMTA Labs Ltd., to be the Affymetrix authorized service provider in India.

Project: Whole Genome Association Analysis of a Ocular Disease using Genome-Wide Human SNP 6.0 arrays-An Indo-Australian collaborative project

This study was aimed to identify DNA variations underlying a Ocular disease predisposition using the Affymetrix Genome-Wide Human SNP 6.0 array which contains more than 1.8 million markers of genetic variation, including single nucleotide polymorphisms (SNPs) as well as probes for the detection of copy number variation. This is an Indo-Australian collaborative project. VIMTA is working on this as a service provider and this is the first project in India using the Affymetrix Genome-Wide Human SNP 6.0 array. This project has two phases, 200 samples in each phase. Further the associated SNP’s will be validated in large number of samples using SEQUENOM MassARRAY Platform.




February 2006 to March 31, 2007: VIMTA Life Sciences, Hyderabad, India

Worked as a Scientist in VIMTA Life Sciences, Hyderabad, India. I have worked in Advanced Molecular Biology (Genomics and Proteomics) Division and performed some pilot work using Affymetrix Microarray platform, SEQUENOM MassARRAY Platform and ABI 4800 MALDI TOF/TOF.

Job Profile: Establishment of Lab, Procurement of instruments, consumables and other raw materials required for lab, preparing the Standard Operating Procedures (SOP’s), standardizing & validating the SOP’s, train the Scientist-Trainees etc...

Pilot Project (In-house funded): RNA transcriptional (Gene Expression) Profiling Using Affymetrix microarray platform: Performed two sets of experiments (four Human Genome Focus Arrays) using Chronic Myeloid Leukemia samples to find out the molecular signatures in CML. Data has been analyzed using ArrayAssist(Avadis) Software. The pathway profiling was done to map the differentially expressed genes on their respective biological, biochemical, Molecular function pathways using GenMAPP2.1 (Gene Map Annotator and Pathway Profiler) software. Similar type of Protein Expression profiling has been done using Proteomics platform to correlate the results.

Development of SNP-Genotyping assay to validate the SNP’s in β- Thalassemia: We are trying to develop an assay to validate the SNP’s in β-Thalassemic genes (HBB, HBD, HBG1, HBG2 etc..) using SEQUENOM MassARRAY platform. It is MALDI-TOF based analysis. I have developed a 23plex assay which can validate 23 SNP’s on HBB gene in a single reaction.

October 2002 to February 2006: CCMB, Hyderabad, India

During this three and half year period, I have worked at Center for Cellular and Molecular Biology (CCMB), Hyderabad, as a Project Research Fellow. In this institute I have worked in “Microarray department” using cDNA microarray and Affymetrix microarray platforms. The first project I was involved at CCMB is New Millennium Indian Technology Leadership Initiative (NMITLI) with the aim for the development of “New targets and markers for cancer using Genomics and Proteomics”, sponsored by Council of Scientific Industrial Research (CSIR), New Delhi. In this project, I studied the differential gene expression patterns of head and neck cancers by cDNA Microarray analysis. We have collaborations with various hospitals in India for tissue bank. The RNA, isolated from tumors and normal tissue samples, is amplified and used for hybridization with 19K Human arrays (University Health network, Micro array Center, Toronto) and with In-House spotted 4K human arrays. We have identified few genes showing significant differential expression patterns, further work is on for validation of these results.

Printing Arrays Experience: I was also involved in the establishment of Human Gene Bank comprising 19,325 human clones and making of custom cDNA arrays as core micro array facility at CCMB. During this work, I got good experience in Culturing EST clones obtained from Incyte and Image consortium, plasmid DNA isolation, purification, PCR, PCR product purification,




spotting on slides (4K) and preparation of gene lists per the spotted slide orientation. These 4K Human arrays were also used to study the differential gene expression profile of head and neck cancers along with 19K Commercial Human arrays (University Health network, Micro array Center, Toronto).

I was involved in the Nutrigenomics project at CCMB to study the effect of a peptide obtained from Soya bean on ACE (Angiotensin Converting Enzyme) treated HEK cell lines using the transcriptomics analyses.

I was also involved in a project titled “Genome wide expression profiling of reversibly arrested myoblasts” with Dr.Jyotsna Dhawan, CCMB, Hyderabad. I have done the data analysis part and the work was fruitful by means of a co-authorship in Plos One.

Global transcriptional profiling of rice in susceptible & resistant interactions with bacterial leaf blight pathogen, Xanthomonaspv. Oryzae. This study was carried out by hybridizing the labeled total RNA of highly resistant line with three resistant genes (Xa21, Xa12, and Xa5) and highly susceptible line (i.e. without any ‘R’ gene) on Agilent 22K Oligoarrays & Affymetrix Rice Genome arrays. I have been involved in data analysis part. Several transcriptional factors and disease response genes were found to be differentially expressed in resistant and susceptible interactions (host plant VsXanthomonaspv. Oryzae). Validation using real-time PCR is in process.

Aug 2008 – Dec 2014 University of Greifswald, Greifswald, Germany

Dr. rer. nat. (Ph.D. in Cardiovascular Biology)

Title of the thesis: Effects of irbesartan and dronedarone on in vivo gene expression signatures of left atrial tissue in a porcine model of acute rapid pacing Date of defense: 10.12.2014

Jun 2001 – Sep 2001 Motive Software Solutions (P) Ltd, Guntur, India

Oracle 8i with D2K, Computer applications

May 2001 – Jun 2001 Compu-U-Learn, Guntur, India

C++, Computer applications

Jun 1999 – May 2001 Acharya Nagarjuna University, Guntur, India

M.Sc. (Master of Science) in Microbiology

Percentage of marks –74.5%.

Educational Qualifications


http://ub-ed.ub.uni-greifswald.de/opus/volltexte/2014/2108/





Apr 1997 – Apr 1998 Independent Coursework @ Baba Computers & Systems, Repalle, India

PGDCA (Post Graduate Diploma in Computer Applications), Computer applications

Jun 1996 – May 1999 V.S.R. & N.V.R. College (Acharya Nagarjuna University), Tenali, Guntur, India

B.Sc. (Bachelor of Science), Biochemistry, Microbiology & Nutrition

Percentage of marks –78.7%.

Jun 1994 – May 1996 N.K.R. Junior College, Repalle, Guntur, India

Class XII, Biology, Physics & Chemistry

Percentage of marks – 83.3%

Genomics

❖ NGS applications: Clinical exome sequencing, WGS (HiSeqx X10), WES (HiSeq 2500/4000), Amplicon Seq, RNA-Seq (HiSeq 4000), small RNA Seq (HiSeq 2500), Targeted sequencing etc.

❖ Microarray gene expression analysis (transcriptome/miRNA) and Whole Genome SNP Genotyping using Affymetrix Microarray Platform.

❖ miRNA profiling using miRCURY LNA™ Serum/Plasma Focus Panels, Exiqon ❖ Microarray expression analysis using Agilent/cDNA microarrays. ❖ SNP Genotyping using SEQUENOM MassARRAY Platform and Fluidigm. ❖ Developing and establishing of cDNA clone bank for custom cDNA chips. ❖ Printing cDNA arrays using Molecular Dynamics Gen-III spotter. ❖ DNA & RNA Isolation, Plasmid DNA procedures ❖ Analysis of DNA, RNA using Agilent 2100 Bio-Analyzer, Conventional Gel electrophoresis

– DNA, and RNA & Proteins. ❖ PCR, PCR product purification ❖ RT-qPCR (Quant Studio 5, ABI 7900 HT) ❖ Semi-Quantitative RT PCR ❖ RNA amplification, In-vitro Transcription ❖ Micro array Probe labeling, Hybridizations ❖ Microarray data analysis

Hands on experience




Proteomics ❖ Protein Extraction, 2D-Gel electrophoresis (IEF, SDS-Page), ❖ Image acquisition and gel analysis using Bio-Rad’s PDQUEST-Advanced software,

Excision of differentially expressed spots using Bio-Rad’s Ex-Quest Spot cutter etc. ❖ In-Gel digestion using Trypsin. ❖ Protein ID identification through PMF using ABI 4800 MALDI TOF/TOF Analyzer & GPS

Explorer ❖ Gel-free proteomic analysis

DNA sequencing (Sanger/NGS)

❖ NGS applications: Clinical exome sequencing, WGS (HiSeqx X10), WES (HiSeq 2500/4000), Amplicon Seq, RNA-Seq (HiSeq 4000), small RNA Seq (HiSeq 2500), Targeted sequencing etc.

❖ Microbial cultures, 16s rDNA sequencing based Microbial type identification using MicroSeq kit from ABI

❖ DNA Sequencing using ABI 3130xl, SSR markers validation

Cell biology ❖ Cell culture: HL-1, HUVEC, SMC, Primary Cardiac Fibroblasts, HEK 293, 3T3-L1, L929 ❖ Viability test using Countess II FL Automated Cell Counter, Biochemical assays ❖ Oxidative phosphorylation measurement in cell lines and tissue

During my M.Sc course, I pursued two months project titled “Preliminary characterization of Cyclodextrin Glycosyl Transferase (CGTase) by Bacillus cereus” at Indian Institute Of Chemical Technology (IICT), Hyderabad, under the guidance of Dr.R.S.Prakasham. Summary of the project work In the thesis work, I have characterized the production of Cyclodextrin Glycosyl Transferase (CGTase) using Bacillus cereus. This enzyme is used in the production of Cyclodexterins from starch. These Cyclodextrins are used as basic materials in variety of industries such as food, cosmetics and pharmaceuticals. I’ve evaluated the role of different carbohydrates as inducing substances for enhanced production of CGTase. The influence of temperature on enzyme production and activity of enzyme was also investigated. During this work, I have used culture techniques, maintenance of stock cultures, enzyme isolation and enzyme assay by Phenolphtalein-β-Cyclodextrin complexation method. At the end of my work I have started purification of enzyme and screening. During the study, I learned biochemical techniques. This project experience has given me exposure to the research atmosphere with stimulating environment.

M.Sc. (masters degree) Thesis




Operating Systems : Windows 10/8/7/VISTA/XP, Windows NT, Mac O.S 10/9.0,

Linux

GWA Data Analysis : PLINK, Haploview, WGA Viewer, SNPtest, CopyColler v1.0

AffymetrixData Analysis : Rosetta Resolver, Genedata Analyst, Array Assist (Avadis),

Ingenuity Pathway Analysis,, Data Assist, AGCC,

GCOSv1.4,GTYPE, Netaffix, CNAT (Copy Number

Analysis Tool),Expression Console, Genotyping Console

3.1etc…...

cDNA Microarray Analysis : Array Vision, Imagequant, Scorecard, Spotfire, TIGR-

MIDAS,TIGR-MeV, TIGR-Spotfinder, MS-Excel, MS-Access.

Pathway Profiling : IPA, GenMAPP v2.1, Panther

Proteomics : Rosetta Elucidator, Proteome Discoverer 1.2, SOURCERER,

Scaffold 3, PDQUEST Advanced, ABI 4000 Series Explorer,

GPS Explorer,MASCOT

Sequence Analysis : Sequencing Analysis 5.2, Gene tool, Bioedit

Office Packages : Graph Pad Prism, MS-office (Excellent command over MS-

Excel, MS-Access), Adobe Photoshop7.0, Macromedia Suit.

Languages : C, C++, FORTRAN, COBOL

RDBMS : ORACLE 8i.

1. Chilukoti RK, Giese A, Malenke W, Homuth G, Bukowska A, Goette A, Felix SB, Kanaan J, Wollert HG, Evert K, Verheule S, Jais P, Hatem SN, Lendeckel U, Wolke C. Atrial fibrillation and rapid acute pacing regulate adipocyte/adipositas-related gene expression in the atria. Int J Cardiol. 2015 Mar 23; 187:604-613. doi: 10.1016/j.ijcard.2015.03.072. [Epub ahead of print]. PMID: 25863735

2. Chilukoti RK, Mostertz J, Bukowska A, Aderkast C, Felix SB, Busch M, Völker U,

Goette A, Wolke C, Homuth G, Lendeckel U. Effects of irbesartan on gene expression revealed by transcriptome analysis of left atrial tissue in a porcine

Data (computational) analytical skills

Peer reviewed publications


http://www.ncbi.nlm.nih.gov/pubmed/25863735




model of acute rapid pacing in vivo. Int J Cardiol. 2013 Oct 3; 168(3):2100-8. Epub 2013 Feb 12. PMID: 23414741

3. Sabine Ameling*, Tim Kacprowski*, RaviKumar Chilukoti*, CarolinMalsch,

VolkmarLiebscher, KarstenSuhre, MaikPietzner, Georg Homuth, Nele Friedrich, Elke Hammer, UweVölker. “Associations of circulating plasma microRNAs with age, sex and BMI in apopulation-based study”. BMC Med Genomics. 2015 Oct 14;8(1):61. doi: 10.1186/s12920-015-0136-7. *: Equal contributing author

4. Richter E, Harms M, Ventz K, Gierok P, Chilukoti RK, Hildebrandt JP, Mostertz J, Hochgräfe F. A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin. PLoS One. 2015 Mar 27; 10(3):e0122089. doi: 10.1371/journal.pone.0122089. eCollection 2015. PMID: 25816343

5. Subramaniam S, Sreenivas P, Cheedipudi S, Reddy VR, Shashidhara LS, Chilukoti RK, Mylavarapu M, Dhawan J. Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs. irreversible arrest. PLoS One. 2013 Jun 3; 8(6):e65097. doi: 10.1371/journal.pone.0065097. Print 2014. PMID: 23755177

6. Warsow G, Endlich N, Schordan E, Schordan S, Chilukoti RK, Homuth G, Moeller MJ, Fuellen G, Endlich K. PodNet, a protein-protein interaction network of the podocyte. Kidney Int. 2013 Jul; 84(1):104-15. doi: 10.1038/ki.2013.64. Epub 2013 Apr 3. PMID: 23552858

7. Kabgani N, Grigoleit T, Schulte K, Sechi A, Sauer-Lehnen S, Tag C, Boor P, Kuppe C, Warsow G, Schordan S, Mostertz J, Chilukoti RK, Homuth G, Endlich N, Tacke F, Weiskirchen R, Fuellen G, Endlich K, Floege J, Smeets B, Moeller MJ. Primary cultures of glomerular parietal epithelial cells or podocytes with proven origin. PLoS One. 2012; 7(4):e34907. doi: 10.1371/journal.pone.0034907. Epub 2012 Apr 18. PMID: 22529955

8. Bukowska A, Hammwöhner M, Sixdorf A, Schild L, Wiswedel I, Röhl FW, Wolke C, Lendeckel U, Aderkast C, Bochmann S, Chilukoti RK, Mostertz J, Bramlage P, Goette A. Dronedarone prevents microcirculatory abnormalities in the left ventricle during atrial tachypacing in pigs. Br J Pharmacol. 2012 Jun; 166(3):964-80. doi: 10.1111/j.1476-5381.2011.01784.x. PMID: 22103242

9. Bis JC, Kavousi M, Franceschini N, Isaacs A, Abecasis GR, Schminke U, Post WS, Smith AV, Cupples LA, Markus HS, Schmidt R, Huffman JE, Lehtimäki T, Baumert



















J, Münzel T, Heckbert SR, Dehghan A, North K, Oostra B, Bevan S, Stoegerer EM, Hayward C, Raitakari O, Meisinger C, Schillert A, Sanna S, Völzke H, Cheng YC, Thorsson B, Fox CS, Rice K, Rivadeneira F, Nambi V, Halperin E, Petrovic KE, Peltonen L, Wichmann HE, Schnabel RB, Dörr M, Parsa A, Aspelund T, Demissie S, Kathiresan S, Reilly MP, Taylor K, Uitterlinden A, Couper DJ, Sitzer M, Kähönen M, Illig T, Wild PS, Orru M, Lüdemann J, Shuldiner AR, Eiriksdottir G, White CC, Rotter JI, Hofman A, Seissler J, Zeller T, Usala G, Ernst F, Launer LJ, D'Agostino RB Sr, O'Leary DH, Ballantyne C, Thiery J, Ziegler A, Lakatta EG, Chilukoti RK, Harris TB, Wolf PA, Psaty BM, Polak JF, Li X, Rathmann W, Uda M, Boerwinkle E, Klopp N, Schmidt H, Wilson JF, Viikari J, Koenig W, Blankenberg S, Newman AB, Witteman J, Heiss G, DuijnCv, Scuteri A, Homuth G, Mitchell BD, Gudnason V, O'Donnell CJ; CARDIoGRAM Consortium. Meta-analysis of genome-wide association studies from the CHARGE consortium identifies common variants associated with carotid intima media thickness and plaque. Nat Genet. 2011 Sep 11; 43(10):940-7. doi: 10.1038/ng.920. PMID: 21909108

10. Heike Wanka, Philipp Lutze, Doreen Staar, Barbara Peters, AnicaMorch, Lukas

Vogel, Ravi Kumar Chilukoti, Georg Homuth, Jaroslaw Sczodrok, Inga Bäumgen and Jörg Peters. "Pro-renin receptor (ATP6AP2) depletion arrests As4.1 cells in the G0/G1 phase, increases formation of primary cilia and prevents mitotic cell division". J Cell Mol Med. 2017 Feb 19. doi: 10.1111/jcmm.13069. PMID: 28215051

11. Ravi Kumar Chilukoti, Josefine Linke, Christian Scharf, Kirsten Utpatel, Katja Evert, Alicja Bukowska, Barbara Peters, Daniel Galla, Uwe Völker, Andreas Goette, Carmen Wolke, Georg Homuth, and UweLendeckel. “Transcriptome and Proteome Characterization of Dronedarone-Treated Myocardium in a Porcine Model of Myocardial Infarction Revealed Predominant Effects of Dronedarone on Post-Infarction Cardiac Healing and Remodeling in the Infarction Border-Zone”. Submitted to Circulation

12. Chilukoti RK, Giese A, A. Bukowska, A. Goette, C. Wolke, J. Kanaan, H.-G. Wollert,

K. Evert, S. Hatem, and U. Lendeckel. “Blockade of Ang-(1-7) Mas receptor prevented Atrial Fibrillation/Rapid Acute Pacinginduced Adipocyte/Adipositas-Associated Gene Expression in the Atria”. In preparation

13. A Bukowska, Ch Hartmann, R K Chilukoti, C Wolke, U Lendeckel, AGoette.

Mediators of oxidative stress in atrial myocardium in Zucker Diabetic Fatty rats. In preparation

14. A. Bukowska, A. Hartmann, R. K. Chilukoti, F. Pluteanu, J. Kockskaemper, C. Wolke, U. Lendeckel, A. Goette. Atrial tachyarrhythmia causes atrial imbalance of




https://www.ncbi.nlm.nih.gov/pubmed/28215051

https://www.ncbi.nlm.nih.gov/pubmed/28215051



the Ace/Ace2 ratio in aged and hypertensive rats. Ready for submission to Circulation Research

15. F. Pluteanu, C. Wolke, A. Bukowska, T. Kiess, C. Sack, R.K.Chilukoti, J. Preisenberger, A. Goette, U. Lendeckel, J. Kockskaemper. Atrial endothelin-1 signalling in hypertensive rats. In preparation

1. W. Malenke, RK. Chilukoti, A. Bukowska, A. Goette, C. Wolke, J. Kanaan, H-G. Wollert, K. Evert, S. Hatem, U. Lendeckel. “Atrial fibrillation and rapid acute pacing provoke induction of adipocyte/adipositas-associated gene expression in the atria”. Accepted for poster presentation at ESC CONGRESS 2014, Barcelona, Spain

2. J. Linke, RK. Chilukoti, C. Scharf, K. Utpatel, A. Bukowska, B. Peters, A. Goette, C. Wolke, G. Homuth, U. Lendeckel. “Effects of dronedarone on myocardial transcriptome and proteome in a porcine model of myocardial infarction”. Accepted for poster presentation at ESC CONGRESS 2014, Barcelona, Spain

3. A. Bukowska, Ch. Hartmann, R.K. Chilukoti, C. Wolke, U. Lendeckel, A. Goette.

“Mediators of oxidative stress in atrial myocardium in Zucker Diabetic Fatty rats”. Accepted for poster presentation at ESC CONGRESS 2014, Barcelona, Spain

4. U. Lendeckel, R.K. Chilukoti, J. Mostertz, A. Bukowska, S.B. Felix, M. Busch, U. Voelker, A. Goette, C. Wolke, and G. Homuth. “Effects of irbesartan on gene expression revealed by transcriptome analysis of left atrial tissue in a porcine model of acute rapid pacing in vivo”. EHRA EUROPACE 2013,, Athens–Greece

5. A. Bukowska, A. Hartmann, R.K. Chilukoti, F. Pluteanu, J. Kockskaemper, C. Wolke,

U. Lendeckel, A. Goette. “Atrial tachyarrhythmia causes atrial imbalance of the Ace/Ace2 ratio in aged and hypertensive rats”. ESC CONGRESS 2013, Amsterdam – Netherlands

6. V. Rami Reddy, SunitaDhawan, Ch.Ravi Kumar, B.Gautami, Sunil Kumar Verma, M.B.

Madhavi and Lalji Singh (2004) “Differential expression profiles in Oral cancers using DNA microarrays”. 8th ADNAT convention: A two-day symposium on “Comparative and Functional Genomics” at CCMB, Hyderabad, India.

❖ ESC CONGRESS 2014, 30 Aug 2014 - 03 Sep 2014, Barcelona - Spain ❖ Workshop on "Genome-wide Association Studies", on 26 November 2008, at Alfried-

Krupp-Wissenschaftskolleg, Greifswald, Germany.

Poster Abstract

Workshops/Symposiums attended




❖ "Course on SNPs and Complex diseases (SNP-V)" during November 3- November 7,

2008 at Erasmus MC, Rotterdam, Netherlands. ❖ “Instructional Workshop in Bioinformatics and Drug Discovery (Bioconvene-2007)”

during December 16 2007 to December 19 2007 at University of Hyderabad, Hyderabad, India.

❖ Indo-French Conference on “RNAi in Genome Control” during December, 12 - 14, 2007 at Centre for Cellular and Molecular Biology, Hyderabad, India.

❖ International Symposium on “Chromosome to Genome” during July, 3 - 5, 2007 at Centre for Cellular and Molecular Biology, Hyderabad, India.

❖ 1st Annual International Conference on "Bench to Bed - 2005: Translational Research for Cancer and Other Diseases" during March 19-20, 2005 at Centre for Cellular and Molecular Biology, Hyderabad, India.

❖ 9th ADNAT symposium on “Molecular Medicine and Health” (20-23 February 2005) at

Centre for Cellular and Molecular Biology, Hyderabad, India. ❖ EMBO workshop on “Cell Interactions in Development and Disease” (December 16th -

18th 2004) at Centre for Cellular and Molecular Biology, Hyderabad, India. ❖ 8th ADNAT convention a two-day symposium on “Comparative and Functional

Genomics” (Feb. 23& Feb. 24, 2004) at CCMB Hyderabad, India. ❖ 7th ADNAT convention “A two day symposium on Proteomics” (Feb. 23& Feb. 24, 2003)

at CCMB Hyderabad, India. ❖ Silver jubilee symposium on “Current Excitement in Biology” (Nov 24-29, 2002) at

CCMB, Hyderabad, India. Nationality : Indian Date of Birth : 20-11-1978 Marital status : Married Address for correspondence: Flat no 101, Sai Ram Palace, 16th A Cross, Neeladri

Road, Electronic City Phase 1, Bangalore- 560100. INDIA.

Permanent Address : H.No.3-31, Peteru Village, Repalle Mandal, Guntur district -522267. INDIA Phone : +91- 99590 13223 (Mobile)

Personal details




E-Mail : [email protected] / [email protected]

Prof. Dr. Uwe Lendeckel Institute of Medical Biochemistry and Molecular Biology, Ernst-Moritz-Arndt University Greifswald Klinikum DZ 7, Ferdinand-Sauerbruch-Strasse D-17475 Greifswald, Germany. E-mail: [email protected] Phone: +49 03834 86-5425

Prof. Dr. Karlhans Endlich Department of Anatomy and Cell Biology Universitätsmedizin Greifswald Friedrich-Loeffler-Str. 23c 17487 Greifswald, Germany. E-mail: [email protected] Phone: +49 03834 86-53 00

Prof. Dr. Uwe Voelker Department of Functional Genomics Ernst-Moritz-Arndt University Greifswald Friedrich-Ludwig-Jahn-Straße 15 a 17487 Greifswald, Germany. E-mail: [email protected] Phone: +49 03834 86-5870

Dr. Ramesh K. Agarwal Group Leader CCMB Hyderabad-500007 India E-mail: [email protected] Phone: +91-40-27192635

Prof. Dr. LS Shashidhara Professor and Coordinator, Biology Indian Institute of Science Education and Research Dr. Homi Bhabha Road Pashan Pune - 411008. (Maharashtra). INDIA E-mail: [email protected]

Dr. Jyotsna Dhawan Group Leader CCMB Hyderabad-500007 India E-mail: [email protected]/ [email protected]

(Ch. Ravi Kumar)

References











Health & Medicine

Cv dr. ravi kumar chilukoti revised_march 2017