Differential LeukocyteCount

Dr.Raghuveer.CR

Objectives

▪ Definition of DLC▪ Identification of WBCs▪ Functions▪ Conditions effecting WBCs count

DLC-Relative proportion of different leukocytes expressed as percentage.

USES:▪ To support the diagnosis of infectious ,

inflammatory or allergic disorders.▪ Diagnosis of malignant blood disorders

Identification-Neutrophils

Neutrophilia-ANC7500/microL

▪ Acute bacterial infections▪ Tissue necrosis▪ Acute blood loss▪ Acute haemorrhage▪ Metabolic disorders▪ Poisoning▪ Malignant tumors

Neutropenia-ANC<2000/microL

▪ Decreased or ineffective production in BM▪ Infections- Typhoid , Miliary Tuberculosis,

measles , rubella , influenza▪ Hematologic disorders▪ Drugs-Analgesics , antithyroid drugs , anti

cancer drugs▪ Ionizing radiation

Increased destruction

▪ Neonatal isoimmune neutropenia▪ Systemic lupus erythematous▪ Felty’s syndrome

Eosinophil

Eosinophilia-AEC>600/microL

▪ Allergic diseases▪ Skin diseases- Eczema , dermatitis herpetiformis▪ Parasitic infection ▪ Carcinoma with necrosis▪ Lung diseases- Loffler's syndrome , tropical

eosinophilia

Basophil

Basophilia- ABC >100/ microL

▪ CML▪ Polycythemia vera▪ Myxedema▪ Drug/food allergies

Monocyte

Monocytosis AMC>1000/micro l

▪ Infections-TB , Sub Acute Bacterial Endocarditis▪ Hodgkins disease▪ Ulcerative colitis , Chrons disease

Small and Large lymphocyte

Lymphocytosis

▪ ALC >4000(adults) >9000(children)Infections- Hepatitis , Cyto megalo virus ,

mumps , rubella , varicella , TB , BrucellosisAML , CML , Multiple myeloma

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Summary

▪ DLC-Relative proportion of leucocytes▪ Neutrophils-40 -60%- Acute infection▪ Lymphocytes-20 – 40%-Viral infection▪ Monocytes: 2 – 8%-Chronic inflammation▪ Eosinophils: 1 – 4%-Allergy and parasitic infection▪ Basophils: 0.5 – 1%--CML

Thank You