Yokogawa Cell Voyager 7000

Wako Automation Introduction and Overview

Wako Automation

Background

Wako Pure Chemicals Japan, USA, Germany

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Wako Diagnostics Japan, USA, Germany

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Wako Automation USA

High Content Imaging Platform

Yokogawa CV7000

High Content Imaging Platform

Automation-Ready

Yokogawa CSU-W1

•Minimum Bleaching and Toxicity •Reduced Crosstalk •Visualize Dynamic Events in Live Cells

Dual Spinning Disk Systems

4X Cells Imaged Reduced Crosstalk Thicker Samples

CSU-W1- Wide View Dual Spinning Disk

Live Cell Imaging

HeLa cell with Azami green, GFP, 10X, 10% 488nm laser 3 days

Yokogawa CSU-X1 Dual Spinning Disk

Live Cell Imaging

Live Cells imaged every 20 minutes for 24 hours

Cell Voyager 7000 Configuration

Simultaneous Imaging with Three (3) Cameras

sCMOS -20˚C 5.5MP, 2560 x 2160 6.5µm pixel

Objective Choices

Choose Up To 6 Lenses Dry: 2x, 4x NA .16, 10x NA .4, 20x NA .75,

40x NA .95, 20x NA .45 LWD !

Water Immersion: 40x NA 1.15, 60x NA 1.2

Phase Contrast: 10X, 20X

Lens Field of View 2x 8.3 mm x7mm 4x 4.15 mm x 3.5 mm 10x 1.66 mm x1.4 mm 20x 0.83 mm x0.7 mm 40x 0.415 mm x0.35 mm 60x 0.276 mm x0.23 mm

sCMOS Camera FOV

Plate TypeWell Size Lens

96 well 6.5mm 2x objective

384 well 3.25mm 4x objective

1536 well 1.5mm 10x objective

sCMOS Camera FOV

HeLa cell mitochondria stained with MitoTracker® Red CMXRos 0.2 um steps, 21 slices, 60X water immersion lens.

Live Cell Imaging

!40X lens, Laser; 561 nm, Laser power; 10%, Exposure time; 20ms, 3 days, 20 min.

Neural Progenitor Cells

Reproducibility of stage Plate removed 6X

Imaged with 60x Water

Imaging Modes

Confocal Epifluorescence Phase Contrast Bright Field

Lasers: Choice of Four

405, 488, 532, 561, 640 !

UV LED: 365 (not confocal)

Halogen Lamp (not confocal)

Neurite Imaging60X W lens, NA 1.2

CV7000 Capabilities

6 to 1536 well plates Microscope Slides

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Incubated Stage 35˚C - 40˚C, CO2, Humidity

Live Cell Imaging !

Laser Based Autofocus Image Based Autofocus

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Dispense Option single channel

for kinetics assays

Image Standard Plate Types & Microscope Slides

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3 colors, 96 wells, <2 min3 colors, 384 wells, <5 min3 colors, 1536 wells, <18 min

(50ms, 1 field, 1 z Stack)

Imaging Speed (3 cameras)

Exceptional Image Quality

Additional Features

Thick Sample Imaging Possible

with CSU-W1

Cy2 Collagen IV (Basement membrane) - green CY3 Pgp9.5 (pan-neuronal Marker)- red

DAPI DNA (Cell nucleuc)- Blue

Exp： 200ms, Z axis 80um , 1.0µm spacing, 81 slices in total　 Objective: 60X

The CV7000 Benefits

Laser  light  focused  through  microlens  Reduced  photo-­‐bleaching  and  photo-­‐toxicity  

Be:er  confocal  images

Yokogawa Dual Spinning Disk System

unmatched speed

4x field of view

live cell imaging for days