Embed Size (px)
Citation preview
Increase Your Multiplexing Capacity for SeqCap
Target Capture
Roche NimbleGen’s SeqCap EZ Library target enrichment system is a...
Solution-based capture method & enables enrichment of the whole exome
or custom regions of interest
One of the biggest drawbacks of the SeqCap EZ Library target enrichment
system is that there are...
Only 24 barcodes and blockers available for multiplexing
And they are supplied lyophilized which need to be resuspended
Bioo Scientific has increased the ease and multiplexing capability of the
SeqCap target capture baits with the...
NEXTflex™ Pre-Capture Combo Kit(NimbleGen SeqCap Compatible)
The NEXTflex™ Pre-Capture Combo Kit offers up to 96, ready-to-use
barcodes and blockers for multiplexing
Increasing the number of samples you can multiplex from 24 to 96
Reducing the cost of targeted sequencing dramatically
The NEXTflex™ Pre-Capture Combo Kit is a complete Illumina-compatible
library prep solution
Which integrates with the SeqCap Target Capture System
Workflow Stage Reagents Needed Bioo Supplied NimbleGen Supplied
1. Library Preparation
NEXTflex DNA-Seq Library Reagents ✓
NEXTflex DNA Barcodes ✓
2. Hybridize Libraries to Probes
NimbleGen SeqCap Target Capture Baits ✓
NEXTflex DNA Barcode Blockers ✓
NimbleGen SeqCap Hybridization & Wash Kit ✓
NimbleGen SeqCap Pure Capture Bead Kit ✓
3. Amplify Captured Libraries NEXTflex Post-capture Amplification Reagents ✓
The kit has also been validated by both Bioo Scientific and Roche for use with
SeqCap Target Capture
Mapping & Coverage Statistics of the NEXTflex™ Pre-Capture Combo Kit
TargetedBases
63,564,965
Mapped Readson Target
75.6%
Raw ReadsMapped
80.0%
AverageCoverage
43.2x
RawReads
76,154,408
Sensitivity DetectingKnown SNPs
96.8%
Unique ReadsMapped
75.5%
Specificity DetectingKnown SNPs
99.2%
Four NEXTflex libraries were prepared from 1 µg of NA19238, NA19239, NA19240, or NA12762genomic DNA with NEXTflex index adapters using standard NEXTflex protocols. EZ Exome v3.0
targets were enriched from these multiplexed libraries.
To learn more about the validation view the whitepaper: An Integrated Solution to
Simplify Library Preparation & Multiplexing for NimbleGen Sequence Capture
DOWNLOAD WHITEPAPER
If you have any questions email us [email protected]
or visit our website atBiooScientific.com/SeqCap
