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BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA Agung Karuniawan Faculty of Agriculture, Padjadjaran University Budi Waluyo Faculty of Agriculture, Brawijaya University 2014 International Workshop Tropical Bio-resources For Sustainable Development, The Role of Innovation to Enhance Germany Alumni in Scientific and Professional Capacities, August 13rd-15rd 2014 Bogor-West Java, Indonesia.

BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

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Page 1: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT

GENETIC RESOURCES OF WEST JAVA INDONESIA

Agung Karuniawan Faculty of Agriculture, Padjadjaran University

Budi Waluyo

Faculty of Agriculture, Brawijaya University

2014

International Workshop Tropical Bio-resources For Sustainable Development, The Role of Innovation to Enhance Germany Alumni in Scientific and Professional Capacities,

August 13rd-15rd 2014 Bogor-West Java, Indonesia.

Page 2: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

INTRODUCTION

• Sweetpotato is an important potential food crop

• Tuber : Main source of carbohydrate, vitamins A (orange flesh tuber), B and C, potassium, phosphorus, calcium, magnesium, antioxidant (purple flesh tuber)

• Leaf : rich in polyphenols, protein, vitamins and minerals

Page 3: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

INTRODUCTION

• Indonesia is one of the area of center diversity sweetpotato in the word

(Mok & Schmiediche 1998)

(Kays et al., 2005)

Page 4: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

INTRODUCTION

• West Java is one of the centers of diversity of sweet potatoes in Indonesia

• People consume sweet potato as food consumption directly through honey sweet potato Cilembu

• Cilembu honey sweet potato has diverse variations and adapt specific regions

• Local honey sweet potato endangered • Padjadjaran University has conducted exploration and

conserve local germplasm accessions of sweet potato in West Java

• Used as a material to increase the genetic capacity on a new type of honey sweet potato

Page 5: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

OBJECTIVE

• Conserving local sweetpotato germplasm in West Java

• Conduct plant breeding based local germplasm accession through polycross in the plot collection

• Select F1 potential for new type honey sweet potato

Page 6: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

MATERIAL AND METHOD

1. Germplasm collection 2. Polycross plot and harvesting seed

Page 7: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

3. F1 evaluation and selection 4. Sweetness test (% Brix)

Page 8: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

RESULT : Breeding Scheme

Germplasm collection and

polycross plot

Seed (known maternal, unknown paternal)

Propagation

Select Clone (4-5 month)

4 month

3 mon th

Select Clone (4-5 month)

Select Clone (4-5 month)

Page 9: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

Distribution Primary Unpad’s Sweetpotato Germplasm Collection Main Characteristics (sample size n=50)

0

2

4

6

8

10

12

14

16

18

Fre

qu

en

cy

Storage Root Shape

0

5

10

15

20

25

Fre

qu

en

cy

Predominant Flesh Color

0

5

10

15

20

25

30

35

Fre

qu

en

cy

Fresh weight perstorage root (g)

0

2

4

6

8

10

12

14

16

18

Fre

qu

en

cy

Yield Potency (t/ha)

0

5

10

15

20

25

Fre

qu

en

cy

% Brix (baked-oven 190oC -2 hour)

Page 10: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

Distribution F1 Plant – seed Obtained from Polycross Plot and

Selection Based on Storage Root Shape Characteristic

68

17

101

66

18 16

232

12

34

0

50

100

150

200

250

Fre

qu

en

cy

Storage Root Shape

Selected storage root shape : 1. Elliptic 2. Oblong 3. Long oblong 4. Long Elliptic New type honey sweetpotato

Page 11: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

Main Characteristic Selected F1 for New Type Honey Sweetpotato

• Fresh tuber weight between 150-300 g tuber -1

elliptic Long

elliptic long

oblong oblong

150

170

190

210

230

250

270

290

310

Fre

sh w

eig

ht

pe

rsto

rage

ro

ot

(g)

Fresh weight perstorage root (g)

elliptic

long elliptic

long oblong oblong

0

10

20

30

40

50

60

70

Yie

ld P

ote

ncy (

t/h

a)

Yield Potency (t ha-1)

elliptic long

elliptic long

oblong oblong

0

5

10

15

20

25

30

35

% B

rix

(bak

ed

)

% Brix (baked-oven 190oC - 2hour

Page 12: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

Selected F1 New Type Honey Sweetpotato Based

% Brix > 10 and Higher Yield > 15t ha-1

Flesh colour No.

Obs.

Yield

(t ha-1)

Tuber weight

(g)

% Brix

Cream 17 16.0 - 51.0 150.0 - 290.0 10.0 - 32.0

Dark yellow 3 22.0 - 31.0 157.1 - 221.4 15.8 - 21.5

Intermediate orange 8 15.0 - 41.0 154.5 - 250.0 11.2 - 22.5

Pale orange 6 18.0 - 38.5 150.0 - 300.0 13.3 - 23.5

Pale yellow 23 16.0 - 43.0 150.0 - 290.9 11.7 - 29.8

Purple 31 15.0 - 62.0 150.0 - 293.8 10.5 - 28.5

White 20 15.5 - 45.5 152.4 - 306.3 10.1 - 28.5

Total 108

Page 13: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

New Types Honey Sweetpotato Varieties

Page 14: BREEDING FOR NEW TYPES OF HONEY SWEETPOTATO BASED ON LOCAL PLANT GENETIC RESOURCES OF WEST JAVA INDONESIA

• Thank You