Cannabis Analyzer for Potency Provides Effortless Determination of Cannabinoid Content

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Topics

1. Interest in Cannabis

2. A brief overview of Cannabis chemistry and potency testing

3. A Turn-Key Potency Solution – The Cannabis Analyzer for Potency

4. What the Cannabis Analyzer solution consists of

5. Goal-oriented Method packages

6. Quantitative accuracy and analytical results

7. Sample preparation example

8. Customer workflow

9. Re-ordering consumables

Shimadzu does not support or promote the use of its products or services in connection with illegal use, cultivation or trade of cannabis products. Shimadzu products are intended to be used for research use only or state approved medical research. Shimadzu Scientific Instruments is not condoning the use of recreational nor medical marijuana.

Interest in Cannabis

Many US states have now legalized cannabis foreither medical or “adult use” (recreational) orboth. Medical cannabis programs exist in overhalf of the US states, and full recreationalmarkets now exist in four states.

Myriad health benefits have been reported forcannabis, including; general pain reduction,anti-nausea, reduction of seizures, and variousaspects of autism.

By 2020, legal cannabis product market salesare expected to surpass $22 billion, with adultuse sales comprising about 53% of the totallegal market.

A Brief Overview of Cannabis Testing

Cannabis contains more than 500 unique compounds,including over 80 chemical alkaloids known ascannabinoids.

QC testing for cannabinoids is essential for the accuratelabeling of cannabis products in both medical andrecreational cannabis markets. Cannabis “potency” isnormally reserved for the quantitation of the majorcannabinoids, principally THCA, THC, CBD, and CBN.

HPLC has emerged as the gold standard for potencydeterminations because separation and detection of thecannabinoids is done without causing any decompositionof the naturally abundant THCA. This acid form undergoesdecarboxylation to THC under the influence of heat andlight.

Naturally Occurring Cannabinoids

...of primary interest for potency determination

A Turn-Key Potency Solution

Choice of 3 Proven HPLC MethodsThree proprietary instrument methods to meet your analytical objectives. No need to spend valuable lab time developing methods – just run samples.

Turnkey HPLC AnalyzerThe package is designed for potency determination through quantitation of the active constituents. It includes all required hardware, software, consumables, and analysis workflow so the analyst is running samples in the shortest possible time.

A complete turnkey HPLC analyzer for quantitative determination of cannabinoid content

3 Years Warranty and Preventive MaintenanceNever worry about whether your potency analyzer will be fully functional and running samples.

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

A Turn-Key Potency Solution

A complete turnkey HPLC analyzer for quantitative determination of cannabinoid content

Target Compound List

Tetrahydrocannabivarin (THCV)

d8-Tetrahydrocannabinoid (d8-THC)

d9-Tetrahydrocannabinoid (d9-THC)

d9-Tetrahydrocannabinolic acid (THCA)

Cannabidiol (CBD)

Cannabidiolic acid (CBDA)

Cannabidivarin (CBDV)

Cannabinol (CBN)

Cannabigerol (CBG)

Cannabigerolic acid (CBGA)

Cannabichromene (CBC)

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

A Turn-Key Potency Solution

High Resolution HPLC Method Package

High Sensitivity HPLC Method Package

High Throughput HPLC Method Package

3 Year Value Plan

Designed for analysis of the 10 most commonly requested cannabinoids in under 8 minutes. This is the original method developed by Shimadzu in collaboration with industry laboratories. (Does not include THCV.)

Adds THCV to the target analyte list, with an instrument cycle time of under 10 minutes. The short analysis time produces the sharpest chromatographic peaks for the best overall sensitivity.

Presents full baseline resolution for all 11 compounds and an analysis time under 30 minutes. This method is preferred for research purposes, or when additional compounds must be added to the analysis in response to new state regulatory requirements.

Provides 3 consecutive and continuous years of full warranty coverage following installation, and scheduled Premium Preventive Maintenance visits.

All three analytical method files are included with the Cannabis Analyzer for Potency Base Package. The three Method Packages include all consumables required to run the method: the analytical

column, package of 5 guard columns, guard column holders, the CRM standard and mobile phase.

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

A Turn-Key Potency Solution

Quality Statement

Mobile phase solvents are pre-mixed (method specific) and UHPLC grade

CRM standard mixtures are certified for quantitative accuracy

Methods are exhaustively tested for:- Ruggedness- Repeatability- Quantitative Accuracy

Goal-Oriented Methods

High Throughput Method

• When high sample throughput is paramount –60 samples per 8 hr. day

• Quantitative for 10 cannabinoids (no THCV)

3.0 4.0 5.0 min0

25

50

75mV

CBD

V

CBD

AC

BGA

CBG C

BD

CBN

d9-T

HC

d8-T

HC

CBC T

HCA

10 mg/L standard mixture

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

Goal-Oriented Methods

2.5 5.0 min0

10

20

30

40

50mV

CBD

V

CBD

A

CBG

AC

BG CBD

THC

V

CBN

d9-T

HC

d8-T

HC CBC T

HCA

High Sensitivity Method

• When THCV is important, yet with good sample throughput – 48 samples per 8 hr. day

• Quantitative for 11 cannabinoids (incl. THCV)

10 mg/L standard mixture

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

Goal-Oriented Methods

10.0 15.0 20.0 min0

25

50

75

mV

CBD

V

THC

V

CBD

CBG CBD

A

CBG

AC

BN

d9-T

HC

d8-T

HC

CBC

THC

A-A

High Resolution Method

• For baseline separation of 11+ components, including THCV. Minimum Resolution = 2.0

• 16 samples per 8 hr. day

• Add cannabinoid targets as regulations change.

10 mg/L standard mixture

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

Standard Curves (High Sensitivity Method)

R2 > 0.999 for all targets

Quantitative Accuracy

Level (ppm) Accuracy % (Bias) RangeLow 2 109.7 – 111.1 ± 1.4Med 20 101.2 – 103.4 ± 2.2High 70 99.2 – 100.2 ± 1.0App Notes

ReportsMethod Package

Column, Guard Columns

Certified Standards

Mobile Phase

High Sensitivity Method

Limits of Detection and Quantitation

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

Target LOQ (mg/L) LOD (mg/L)CBDV 0.43 0.14CBDA 0.55 0.18CBGA 0.53 0.18CBG 0.59 0.20CBD 0.59 0.20THCV 0.62 0.20CBN 0.37 0.12d9-THC 0.52 0.17d8-THC 0.64 0.21CBC 0.60 0.20

All values correspond to Dry WT % of less than 0.1%

Analytical Results

Quantitative Example:DRY WT% = [Target] x (Dilution) x (Extraction Vol./Dry wt mg) x 100

THCA WT% = 3.8% = [18.9 mg/L] x(20/1) x (0.02 L/200 mg) x 100d9-THC WT% = 2.0% = [10.2 mg/L] x(20/1) x (0.02 L/200 mg) x 100

@ 10 mg/L

App NotesReports

Method Package

Column, Guard Columns

Certified Standards

Mobile Phase

Sample Preparation Guidance

• Weigh 200 mg of flower or leaf cuttings into 50 mL centrifuge tube.

• Add two 9.5 mm steel balls into the tube. • Shake at 1000 rpm for 1 min. with the 2010 Geno/Grinder. • Add 20 mL of methanol to the tube.• Shake at 1000 rpm for 1 min.• Wait for 15 min.• Mix using a vortex mixer for 1 min.• Transfer 1 mL of the mixture into a 1.5 mL micro-tube and

centrifuge at 3000 rpm for 5 min.• Transfer 100 μL of supernatant to a new 1.5 mL micro-tube.• Add 900 μL of methanol.• Filter the mixture through a 0.45 μm syringe filter and transfer

to a 1.5 mL sample vial.

Extracts from flowers, leaves, stems

User Workflow

1. Setting samples in the Analysis Screen

Standards set up automatically according to the method

Sample NameSample IDDry Wt Amt.Extraction Vol.Dil. Factor

Unknowns to be analyzed

User Workflow

2. Visual Monitor of runs progressing with the Analysis Screen

User Workflow

3. Quick access to quantitative reports

Double-click on completed run to show report

Re-ordering Consumables

Re-ordering Consumables

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