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Slides describing research published in PNAS, and BMC Evolutionary Biology. We found that light producing photophores evolved separately in two distantly related squid. Surprisingly, overall gene expression is amazingly similar.
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Cephalopod’s Tale(PhD thesis of Sabrina Pankey)
What are bacteriogenic photophores, what do they do?
They evolved more than once in cephalopods
Transcriptomes of convergent photophores are unexpectedly,predictably similar
What If…We Could “Replay the Tape””
Does molecular convergence follow phenotypic convergence?
What are bacteriogenic photophores, what do they do?
Photophores of Squid House a Vibrio symbiosis
Physiological(Response to light)Morphological
Gq
R-opsin
TRP
PLC
Phototransduction Genes
Tong,Rosas,Oakley,Mitchell,Colley,McFall-Ngai. PNAS (2009)
Transcription factors Pax6, Dac, Eya, Six
Peyer, Pankey, Oakley,McFall-Ngai. Mech Dev (2014)
Bacteriogenic photophores evolved more than once.
A phylogenetic hypothesis for convergent origins
A phylogenetic hypothesis for convergent origins
Distantly related squid harboring bioluminescent symbionts
Distantly related squid harboring bioluminescent symbionts
Distantly related squid harboring bioluminescent symbionts
Distantly related squid harboring bioluminescent symbionts
Overall gene expression in convergent photophores is unexpectedly similar.
How does overall gene expression in convergent traits compare to expression in
homologous traits?
Most variation in gene expression is species differences
Photophores cluster together based on gene expression
Convergent Transcriptomes as similar as Homologous Transcriptomes
Pankey et al PNAS (2014)
Conclusions
A ‘Replay of the Tape’ yielded remarkably similar outcomes
Does molecular convergence follow phenotypic convergence?
