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In the development of cancer immunotherapies, vaccines, and autoimmune disease treatments, researchers are increasingly aware of the importance of host human leukocyte antigens (HLA) in understanding the complexity of immune responses. HLA allelic variants have distinct binding affinities for peptide antigens during presentation, and thus regulate auto-antigen and pathogen-specific T cell activation. HLA polymorphisms have been correlated with disease risk, immune responses to infectious disease, as well as adverse responses to therapies. In this webinar, we will review the genetics and biology of HLA types and explain the technology underlying HLA identification. We will also provide some examples of how HLA-typing can be a valuable investment during early stage drug-development in human primary cells.
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Your Reliable Source of Human Primary Cells
Donor HLA Typing 101:How HLA Characterization Can Accelerate Your Research
HLA – What is it?
HLA typing technology
Applications
Q&A
1
2
3
4
Macrophage or APC
B cells
foreign protein
peptide / HLA
T cells
Activated T cells
60-sec Immunology Review
ER
Major Histocompatibility
Complex (MHC)
Antigen-specific T cell
T cell Receptor (TCR)
Human Leukocyte Antigen (HLA)
HLA molecules “present” peptide to T cells
CD4 T helper cell
HLA Class IIHLA Class I
CD8+ Cytotoxic T cell
Cytosolic proteins
Proteosome
All nucleated cells
Extracellular pathogens
(except cross-presentation)
Lysosome
Only APCs
13-25 aa 9-22 aa
2 flavors of HLA
CLA
SS I
1
CLA
SS II
HLA diversity is the result of genetic polymorphisms
HLA-B8
*0801*0802*0803
….. *0821
HLA- B > 3,000 known alleles
• Crossmatch
• Microcytotoxicity Test
• Mixed Lymphocyte Reaction Assay
• Immunophenotyping
• SSP Genotyping
• SSO Genotyping
Evolution of HLA Typing
SSO Genotyping Method
HLA Data Analysis
Transplantation
Forensics
Disease-related Biomarkers
HLA-typing applications
Vaccine Development
HLA and Autoimmune disease
Disease HLA Allele
Celiac Disease HLA-DQ8, -DQ2
Type I Diabetes HLA-DRB1*04:01
Multiple Sclerosis HLA-DRB1*15:01
RA (ankylosingspondylitis)
B27
Behçet's disease HLA-B51
Transplantation
Forensics
Disease-related Biomarkers
HLA-typing applications
Immunogenicity
Vaccine Development
HLA and Immunogenicity
• HLA-B*57:01
Ostrov et al, PNAS, 2012
AllCells HLA-typed
Primary Cells*AllCells
Immunoassays
PBMC
T cells
B cells monocytes
Cytokine Release Assays
MLR Assays
T cell proliferation
T helper cell polarization
HLA typing at AllCells
* Primary cells from healthy donors or diseased patients
Bruce Sun
Sr. Manager, Product and Application Development
Please direct offline questions to [email protected] or
www.allcells.com/technical-support/
www.allcells.com/support/webinars/
Contact Information