Result

“Integrin alpha5 appears in discrete puncta at the tip of protrusions in migrating heart endocardial cells, similar to seen in cell cultures. There is non-specific staining in the blood cells.” F. Guzman - External Laboratory.

Cell Line: Zebrafish embryos

Method of validation: Immunofluorescence (IF)

Primary Antibody: STJ97284 Anti-Integrin α5

antibody

Secondary Antibody Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568

Antigen Retrieval Low concentration of Pro-

teinase K

Dilution ratio: 1:500

Antibody Customer Review: STJ97284 Anti-Integrin α5 antibody Antibody Specificity:

Antibody Rating:

Protocol

Treatment of materials: Zebrafish embryos fixated with 4% paraformaldehyde.

Antigen retrieval: Low concentration of Proteinase K.

Blocking: 10% sheep serum, 1% BSA.

Primary antibody probing: primary antibody was used at 1:500 concentration, overnight at 4C.

Membrane wash: 1X TBST wash for 3 times.

Secondary antibody probing: secondary antibody was used at 1:500 concentration, overnight at 4C.

Membrane wash: 1X TBST wash for 3 times.

Figure

Integrin alpha5 is present in discreet puncta (likely represent focal adhesion complexes) at the tip of protrusions in zebrafish heart endocardial cells (overview and close up attached) .