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Result Strong signal seen mouse photoreceptor outer and inner segment - Cecilia Grestner, University of Utah Cell Line: Mouse rena secon Method of validaon: Immunofluorescence (IF) Primary Anbody: STJ92654 An-DAPK3 An- body Secondary Anbody Alexa488-conjugated Goat- an-Rabbit IgG Diluon rao: 1:300 Protocol Treatment of materials: Blocking: 2% BSA. Primary anbody probing: 1:300 diluon rao, incubated overnight at 4C. Secondary anbody probing: 1:1000 diluon raon, incubated 1 hour at room temperature. Anbody Customer Review: STJ92654 An-DAPK3 Anbody Anbody Specificity: Anbody Rang: Figure 1: Signal seen photoreceptor in outer segment, inner segment and syn- apses .

Immunofluorescence Antibody Customer Review for Anti-DAPK3 Antibody (STJ92654)

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Result “Strong signal seen mouse photoreceptor outer and inner segment ” - Cecilia Grestner, University of Utah

Cell Line: Mouse retina section

Method of validation: Immunofluorescence (IF)

Primary Antibody: STJ92654 Anti-DAPK3 Anti-

body

Secondary Antibody Alexa488-conjugated Goat-

anti-Rabbit IgG

Dilution ratio: 1:300

Protocol

Treatment of materials:

Blocking: 2% BSA.

Primary antibody probing: 1:300 dilution ratio, incubated overnight at 4C.

Secondary antibody probing: 1:1000 dilution ration, incubated 1 hour at room temperature.

Antibody Customer Review: STJ92654 Anti-DAPK3 Antibody Antibody Specificity:

Antibody Rating:

Figure 1: Signal seen photoreceptor in outer segment, inner segment and syn-apses .