Impact Of Electromagnetic radiations of biology and behaviour of Apis mellifera L

Embed Size (px)

Citation preview

  1. 1. Impact Of Electromagnetic radiations of biology and behaviour of Apis mellifera L. Shweta Patel 42537
  2. 2. INTRODUCTION Honeybees play a vital role in the pollination of agricultural crops In recent years honey bee colony declines have reached 10-30% in Europe, 30% in the United States and up to 85% in Middle East, but such declines are not apparent in South America, Africa and Australia (Kluser,2010) The syndrome behind honeybee population decline is known generally as Colony Collapse Disorder (CCD), which is characterized by a rapid loss of adult worker bees (van (Engelsdorp, 2009) EMFs are mentioned as a cause of honeybee colony losses, the sudden disappearance of honeybees, which is a huge problem in the field of beekeeping (VanEngelsdorp et al,2009)
  3. 3. There are several factors for CCD and they fall into two main classes: living organisms human activity Electromagnetic radiation can best be described as waves of electric and magnetic energy moving together (i.e. radiating ) through space . Electromagnetic fields (EMF) are physical fields that are produced by a source of EMR. Electric fields are associated with electric charge and magnetic fields with the movement of electric charge.
  4. 4. Radiation Pattern of a Cell Tower Antenna People living within 50 to 300 meter radius are in the high radiation zone (dark blue) and are more prone to ill-effects of electromagnetic radiation
  5. 5. Proportion of studies on different groups of organisms Birds; 3% Bees; 1% Wildlife;2% Other Animals; 12% Plants; 1%Human; 81% Bees Wildlife Other Animals HumanPlantsBirds Studies on EMF impacts
  6. 6. MATERIALS AND METHODS Fifteen boxes of bees were place at specified locations. First three boxes were placed below the mobile tower. After measuring 200m, 400m, 600m and 800m distance from the tower, three boxes were placed at each location, respectively. At each place 3 replications was taken. 6 Treatment was considered at every place. Assuming first treatment as a no. of bees (bees-strength), second, third, fourth, fifth and sixth as area of honey, egg, larva ,brood and pollen. Each were be measure after 14 days interval. The experiment was divided into 5 spot with each spot different distances: Spot 1: 0 m Spot 2: 200 m Spot 3: 400 m Spot 4: 600 m Spot 5: 800 m
  7. 7. Experimental Site
  8. 8. Radio Frequency Meter
  9. 9. Effect of Radiations on honey area (cm2 ) of honeybees during 2012 Treatment 30.6.12 14.7.12 28.7.12 11.8.12 25.8.12 8.9.12 22.9.12 6.10.12 Mean E (mV/m) B(A/m) S (mW/m2 ) T1 (0 m) 275.833 (16.575) 665.000 (25.672) 277.500 (16.442) 387.500 (19.611) 478.333 (21.049) 745.833 (26.631) 554.166 (22.351) 616.666 (23.693) 500.101 (21.503) 304.297 541.22 302.913 T2 (200 m) 606.666 (24.300) 789.166 (27.588) 473.333 (21.448 521.666 (22.684) 372.500 (19.299) 305.000 (17.386) 308.333 (17.454) 514.166 (22.36) 486.35 (21.564) 10.663 24.08 0.248 T3 (400 m) 361.400 (18.346) 710.000 (26.442) 530.000 (22.799) 432.500 (20.333) 397.500 (19.686) 912.500 (28.609) 854.166 (28.753) 96.666 (9.791) 536.84 (21.844) 20.826 109.54 2.235 T4 (600 m) 500.833 (22.082) 798.333 (27.963) 455.833 (20.885) 370.833 (18.236) 542.500 (22.552) 460.833 (19.872) 407.333 (19.458) 234.633 (14.918) 471.388 (20.745) 7.198 19.338 0.0981 T5 (800 m) 645.000 (25.346) 733.333 (26.958) 488.333 (21.990) 527.500 (22.940) 190.623 (13.626) 883.316 (29.694) 592.083 (24.304) 347.500 (18.535) 550.958 (22.924) 7.088 19.12 0.132 Mean 477.946 (21.330) 739.166 (26.925) 445.000 (20.713) 448.000 (20.761) 396.291 (19.243) 661.496 (24.439) 543.216 (22.464) 361.926 (17.859) 509.130 (21.716) _ _ _ Sem 107.523 (2.635) 148.275 (2.851) 105.073 (2.5687) 102.057 (2.6173) 112.528 (2.828) 255.034 (4.9305) 163.145 (3.6768) 124.922 (3.026) _ _ _ _ Cd at 5% 350.449 (8.589) 483.270 (9.294) 342.466 (8.372) 332.633 (8.530) 366.763 (9.218) 831.228 (16.070) 163.145 (11.984) 407.156 (9.863) _ _ _ _ Cv 38.965 (21.40) 34.744 (18.344) 40.897 (21.480) 39.457 (21.835) 49.182 (25.458) 66.777 (34.943) 52.019 (28.349) 59.783 (29.350) _ _ _ _ F value ns ns ns ns ns ns ns ns _ _ _ _
  10. 10. Honey area at different distance from tower during the year 2012
  11. 11. Progressive increase in Honey area during the year 2012
  12. 12. Method estimating the area of honey, egg, pollen, larva and brood
  13. 13. Effect of Radiations on the egg laying capacity in area (cm2 ) of queen bee at Pantnagar during 2012 Treatment 30.6.12 14.7.12 28.7.12 11.8.12 25.8.12 8.9.12 22.9.12 6.10.12 Mean E(mV/m ) B(A/m) S(mW/m2 ) T1 (0 m) 126.666 (9.060) 15.000 (3.142) 157.500 (11.742) 167.500 (12.598) 152.500 (10.018) 95.833 (7.426) 144.166 (10.863) 150.000 (11.258) 126.143 (9.513) 304.297 541.22 302.913 T2 (200 m) 210.833 (14.059) 59.166 (6.796) 199.583 (13.824) 185.833 (13.547) 220.000 (14.803) 145.000 (11.954) 168.333 (11.864) 176.666 (10.808) 170.673 (12.206) 10.663 24.08 0.248 T3 (400 m) 0.000 (0.000) 48.333 (6.915) 215.000 (14.192) 200.833 (13.967) 145.833 (11.842) 45.833 (5.202) 261.666 (15.408) 102.500 (10.055) 127.497 (9.697) 20.826 109.54 2.235 T4 (600 m) 122.500 (8.364) 37.916 (6.052) 203.333 (11.639 140.000 (11.512) 75.000 (6.921) 115.833 (10.305) 105.000 (8.162) 115.833 (10.394) 114.425 (9.168) 7.198 19.338 0.0981 T5 (800 m) 61.666 (4.533) 34.166 (5.824) 100.000 (7.8867) 65.833 (5.712) 125.833 (11.205) 64.166 (6.540) 80.000 (8.7561) 47.833 (6.562) 72.433 (7.127) 7.088 19.12 0.132 Mean 104.333 (7.203) 38.916 (5.746) 175.083 (11.857) 152.000 (11.467) 143.833 (10.958) 93.333 (8.285) 151.833 (11.010) 118.566 (9.815) 122.237 (9.542) _ _ _ sem 74.773 (4.292) 19.006 (1.374) 82.463 (3.960) 51.320 (2.622) 50.919 (3.154) 47.522 (3.200) 71.286 (3.164) 59.257 (3.145) _ _ _ _ Cd at 5% 243.706 (13.991) 61.946 (4.480) 268.770 (12.909) 167.266 (8.546) 165.961 (10.280) 154.888 (10.432) 232.342 (10.312) 193.137 (10.250) _ _ _ _ Cv 124.131 (103.214) 84.589 (41.435) 81.578 (57.860) 58.479 (39.604) 61.317 (49.853) 88.190 (66.912) 81.320 (49.772) 86.564 (55.497) _ _ _ _ F value ns ns ns ns ns ns ns ns _ _ _ _
  14. 14. Egg laying by Queen bee at different distance from tower during the year 2012
  15. 15. Progressive increase in area of egg laying during the year 2012
  16. 16. Impact of Radiations on the brood area (cm2 ) of honeybees at Pantnagar during the year 2012Treatment 30.6.12 14.7.12 28.7.12 11.8.12 25.8.12 8.9.12 22.9.12 6.10.12 Mean E(mV/m ) B(A/m) S (mW/m2 ) T1 (0 m) 109.166 (10.101) 161.449 (20.812) 281.666 (15.901) 198.333 (13.966 283.666 (14.336) 450.833 (17.861) 355.833 (16.619) 574.166 (23.838) 340.817 (16.679) 304.297 541.22 302.913 T2 (200 m) 189.166 (13.750) 435.416 (20.695) 389.583 (19.683) 453.333 (21.133 360.000 (18.883) 451.666 (21.131) 721.666 (26.608) 250.833 (14.163) 406.453 (19.505) 10.663 24.08 0.248 T3 (400 m) 314.166 (17.5487) 570.000 (23.688) 333.333 (18.175) 468.333 (21.585) 441.666 (20.974) 435.000 (20.812) 407.500 (19.234) 578.333 (23.901) 443.531 (20.739) 20.826 109.54 2.235 T4 (600 m) 261.666 (16.084) 553.750 (23.500) 223.541 (13.415) 505.000 (22.441) 204.166 (11.655) 635.833 (25.094) 490.833 (20.054) 587.500 (23.716) 432.772 (19.494) 7.198 19.338 0.0981 T5 (800 m) 164.166 (10.461) 339.166 (17.825) 252.500 (15.869) 286.666 (13.573) 240.833 (14.539) 225.833 (14.991) 215.833 (13.962) 245.000 (15.616) 246.246 (14.604) 7.088 19.12 0.132 Mean 207.666 (13.589) 474.250 (21.304) 296.125 (16.609) 382.333 (18.54) 306.066 (16.078) 439.833 (19.978) 438.333 (19.295) 447.166 (20.247) _ _ _ _ sem 49.535 (2.647) 113.819 (2.634) 85.661 (3.036) 87.997 (3.455) 100.027 (4.431) 122.459 (3.796) 143.272 (4.560) 88.835 (2.545) _ _ _ _ Cd at 5% 161.449 (8.629) 370.969 (8.587) 279.196 (9.896) 286.809 (11.263) 326.017 (14.443) 399.130 (12.373) 466.967 (14.864) 289.540 (8.297) _ _ _ _ Cv 41.315 (33.744) 41.568 (21.420) 50.104 (31.663) 39.864 32.284 56.605 (47.738) 48.224 (32.912) 56.613 (40.937) 34.409 (21.777) _ _ _ _ F value ns ns ns ns ns ns ns ns _ _ _ _
  17. 17. Area of Brood at various distance from tower during the year 2012
  18. 18. Progressive increase in brood area during the year 2012
  19. 19. Impact of Radiations on the larva area (cm2 ) of honeybees at Pantnagar during the year 2012 Treatment 30.6.12 14.7.12 28.7.12 11.8.12 25.8.12 8.9.12 22.9.12 6.10.12 Mean E(mV/m ) B(A/m) S(mW/m2 ) T1 (0 m) 25.833 (3.865) 21.166 (3.554) 138.333 (10.561) 183.333 (12.959) 82.500 8.891) 146.666 (9.878) 220.000 (11.988) 169.166 (12.219) 123.37 (9.239) 304.297 541.22 302.913 T2 (200 m) 7.500 (2.017) 17.466 (4.432) 198.333 (13.982) 194.166 (13.738) 195.000 13.769) 213.333 (14.318) 251.666 (15.576) 164.166 (10.455) 155.2 (11.035) 10.663 24.08 0.248 T3 (400 m ) 201.666 (13.680) 37.500 (6.046) 196.666 (14.011) 215.000 (14.284) 188.333 13.578) 70.000 (6.573) 273.333 (16.448) 263.333 (16.165) 180.726 (12.598) 20.826 109.54 2.235 T4 (600 m) 51.666 (5.849) 40.833 (6.175) 134.166 (9.291) 340.833 (17.956) 179.166 10.407) 124.166 (8.973) 122.500 (8.979) 255.000 (15.370) 156.037 (10.375) 7. 19.338 0.0981 T5 (800 m) 102.500 (7.142) 13.750 (3.700) 109.166 (7.625) 40.833 (5.195) 27.500 (5.210) 39.166 (5.042) 92.500 (8.922) 39.166 (5.9387) 58.07 (6.0968) 7.088 19.12 0.132 Mean 77.833 (6.511) 26.143 (4.782) 155.333 (11.094) 194.833 (12.826) 134.500 (10.371) 118.666 (8.957) 192.000 (12.382) 178.166 (12.029) 134.684 (9.869) _ _ _ sem 56.032 (2.962) 26.143 (1.044) 74.674 (3.793) 74.950 (2.828) 63.498 (2.881) 51.631 (2.953) 66.675 (3.516) 62.312 (2.512) _ _ _ _ Cd at 5% 182.626 (9.656) 32.051 (3.404) 243.385 (12.364) 244.286 (9.217) 206.961 (9.392) 168.280 (9.624) 217.313 (11.462) 203.094 (8.188) _ _ _ _ Cv 124.691 (78.809) 65.151 (37.831) 83.265 (59.226) 66.630 (38.189) 81.772 (48.123) 75.360 (57.102) 60.148 (49.191) 60.577 (36.174) _ _ _ _ F value ns ns ns ns ns ns ns ns _ _ _ _
  20. 20. Area of Larva at different distance from tower during the year 2012
  21. 21. Area of comb occupied by the larvae during the year 2012
  22. 22. Impact of Radiation on the pollen area at Pantnagar during the year 2012 Treatment 30.6.12 14.7.12 28.7.12 11.8.12 25.8.12 8.9.12 22.9.12 6.10.12 Mean E(mV/m) B(A/m) S (mW/m2 ) T1 (0 m) 105.000 (8.856) 13.581 (3.092) 51.456 (6.995) 19.166 (4.136) 62.500 (7.146) 96.666 (9.643) 127.500 (11.245) 159.166 (12.342) 79.376 (7.931) 304.297 541.22 302.913 T2 (200 m) 216.666 (11.93) 1.041 (0.831) 57.500 (7.541) 89.166 (9.197) 129.166 (11.127) 165.000 (12.772) 121.666 (10.987) 140.000 (11.722) 110.52 (9.512) 10.663 24.08 0.248 T3 (400 m) 61.666 (7.7199) .250 (0.288) 46.250 (6.086) 41.250 (6.266) 96.666 (9.623) 59.500 (7.029) 277.500 (15.765) 103.333 (10.160) 85.8 (7.867) 20.826 109.54 2.235 T4 (600 m) 71.666 (8.401) 19.583 (2.900) 50.833 (7.074) 76.750 (8.680) 95.000 (9.577) 69.166 (8.257) 200.000 (14.090) 185.000 (13.370) 95.997 (9.043) 7.198 19.338 0.0981 T5 (800 m) 289.166 (15.634) .1233 (0.202) 35.623 (5.725) 40.400 (5.661) 16.666 (3.329) 18.333 (4.217) 33.7500 (5.4377) 10.873 (2.998) 55.613 (5.400) 7.088 19.12 0.132 Mean 148.833 (10.508) 6.916 (1.463) 48.332 (6.684) 53.346 (6.788) 80.000 (8.160) 81.733 (8.384) 152.083 (11.505) 119.674 (10.118) 373.971 (18.205) _ _ _ sem 100.347 (3.687) 10.165 (1.257) 17.250 (1.266) 20.632 (1.337) 30.269 (1.840) 19.536 (1.149) 50.814 (2.013) 25.831 (1.042) _ _ _ _ Cd at 5% 327.059 (12.019) 33.133 (4.097) 56.223 (4.126) 67.247 (4.360) 98.657 (5.998) 63.675 (3.747) 165.617 (6.562) 84.191 (3.396) _ _ _ _ Cv 116.779 (60.783) 254.596 (148.82) 61.818 (32.804) 66.989 (34.133) 65.535 (39.064) 41.401 (23.752) 57.871 (30.310) 37.385 (17.839) _ _ _ _ F value ns ns ns ns ns ** * ** _ _ _ _
  23. 23. Pollen area at different distance from tower during the year 2012
  24. 24. Progressive increase in Pollen area of comb during the year 2012
  25. 25. Impact of radiations on the Bees-strength of honeybees at Pantnagar during the year 2012 Treatment 30.6.12 14.7.12 28.7.12 11.8.12 25.8.12 8.9.12 22.9.12 6.10.12 Mean E(mV/ m) B (A/m) S (mW/m2 ) T1 (0 m) 19832.33 (136.754) 23000.00 (151.452) 23334.33 (150.975) 26962.33 (161.410) 9997.33 (98.946) 19719.67 (139.416) 20775.67 (139.811) 26053.33 (153.728) 21209.37 (141.562) 304.297 541.22 302.913 T2 (200 m) 21777.67 (147.485) 17000.00 (130.178) 20222.33 (141.621) 23388.67 (152.118) 27109.00 (164.536) 20887.67 (143.053) 23331.67 (152.678) 29941.67 (167.983) 22957.34 (149.957) 10.663 24.08 0.248 T3 (400 m) 22333.33 (148.830) 23055.33 (151.298) 87889.00 (254.890) 26443.33 (161.528) 28442.67 (167.184) 24053.67 (154.429) 24553.00 (156.530) 26479.67 (158.521) 32906.25 (169.151) 20.826 109.54 2.235 T4 (600 m) 20666.67 (142.159) 22000.33 (147.175) 28556.00 (168.252) 26388.33 (161.594) 26442.33 (161.783) 28998.33 (169.062) 17442.67 (131.90) 27276.00 (164.409) 24721.33 (155.792) 7.198 19.338 0.0981 T5 (800 m) 16333.33 (122.531) 14333.67 (114.536) 10333.33 (98.469) 13777.33 (117.182) 12058.33 (109.691) 12111.00 (109.934) 10886.33 (104.302) 9720.67 (98.483) 12444.25 (109.391) 7.088 19.12 0.132 Mean 20188.67 (139.552) 19877.87 (138.928) 34067.00 (162.941) 23392.00 (150.786) 20809.93 (140.428) 21154.07 (143.179) 19397.87 (137.044) 23894.27 (148.625) 22847.71 (145.185) _ _ _ sem 5138.29 (19.088) 3752.13 (14.963) 29613.43 (50.973) 4771.11 (14.625) 2914.88 (8.986) 3814.267 (12.755) 553.77 (12.255) 8397.07 (25.921) - _ _ _ Cd at 5% 16747.17 (62.215) 12229.30 (48.768) 96518.68 (166.137) 15550.43 (47.669) 9500.46 (29.290) 12431.79 (41.575) 11582.76 (39.943) 27368.47 (84.484) - _ _ _ Cv 44.083 (23.691) 32.694 (18.654) 150.562 (54.184) 35.327 (16.80) 24.261 (11.084) 31.230 (15.430) 31.731 (15.488) 60.868 (30.208) - _ _ _ F value ns ns ns ns ** ns ns ns - _ _ _
  26. 26. Method of estimating the Bees- Strength
  27. 27. Impact of radiations on bee-strength at different distances from tower during 2012
  28. 28. Progressive increase in number of bees during the year 2012
  29. 29. Radiations at different spots
  30. 30. Radiation at 0 m (beneath,the mobile tower) during the year 2012
  31. 31. RESULTS AND DISCUSSION Bees-strength in the starting of experiment in T1 (0 m, beneath, the mobile tower) was found to be 19,832.33 while, it was seen increased 26,053.33 at the end of experiment, the first week of October, under the influence of electromagnetic radiations emitted from the mobile tower. The overall progressive increased in honey area recorded during the month of June-October, Area of comb, filled with honey was increased 340.833 cm2 in T1 (0m, beneath, the mobile tower) while, decreased in -297.5 cm2 in T5 (800m) followed by T4 (600 m) in 266.2 cm2 , -264.734 cm2 in T3(400m) and -92.5cm2 in T2(200m).
  32. 32. On the basis of observations recorded, it was revealed that the area of egg was significantly increased in T3 (T1102.5 cm2 ) and T1 (23.334 cm2 ) whereas, it was found to be decreased in T2 (-34.167 cm2 ), T5 (-13.836 cm2 ) and T4 (- 6.667 cm2 ). The radiation was recorded during month of June- October. Highest radiation was observed in T1 (E =304.297 mV/m, B =541.22 A/m and S =302.913 m W/m2 ) while minimum in T5 (E =7.088 mV/m, B =19.12 A/m and S =0.132 m W/m2 ) followed by T4 (E =7.198 mV/m, B =19.338 A/m and S =0.0981 m W/m2 ), T2 (E=10.663 mV/m, B= 24.08 A/m and S =0.248 m W/m2 ) and T3 (E =20.826 mV/m, B =109.54 A/m and S=2.235 m W/m2 ), respectively.
  33. 33. Conclusion As per the present investigation, all treated colonies with mobile tower radiation had perform well as normal colony and none of these colonies were perished during the experiment. At 0 m (beneath,the mobile tower) area of honey, brood, larva, egg, pollen all were increased. The present investigation would help to bee- keepers of Tarai region of Uttrakhand where, their bee hives were placed near the mobile tower .No impact of electromagnetic radiations of colony strength and bees survivorship.
  34. 34. Thanks