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Silene ciliata (Caryophyllaceae) as a model species in a climate change scenario: our multidisciplinary
approaches
García-Fernández, A., Morente-López, J., Lara-Romero, C., Rubio-Teso, M.L., Iriondo, J.M.
Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, E-28933 Móstoles, Madrid, Spain.
10 years with Silene ciliata in 10 minutes using 10 slides
García-Fernández, A., Morente-López, J., Lara-Romero, C., Rubio-Teso, M.L., Iriondo, J.M.
Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, E-28933 Móstoles, Madrid, Spain.
• Circum-mediterranean alpine chamaephyte• Elevation range: 1500 – 2500 m• 2 important features: • Polyploid karyotypes
– 2n = 24 to 2n = 192
• Phenotype variability• Description of subspecies:
– Subsp. graffieri (Eastern mountains)– Subsp. ciliata (Western mountains)– Other subsp. and varieties
Silene ciliata Pourret
Introduction Past studies Current researches Future prospects
Silene ciliata Pourret
Introduction Past studies Current researches Future prospects
• Plastid markers support the existence of two subspecies
• More haplotype diversity in western group• Vicariance processes, dispersal barriers and
geographical disconnection associated to glaciation-interglaciation periods
Kyrkou et al. (2015) PeerJ
Introduction Past studies Current researches Future prospects
Silene ciliata Pourret• During the last decade, more than 10 papers
published with the species.• Mediterranean mountains, hotspot of
biodiversity and endemisms.• Altitudinal gradients summarize environmental
variabilities.
Introduction Past studies Current researches Future prospects
Silene ciliata Pourret• Floral asynchrony
Intermediate population
Low population
High population
Gimenez-Benavides et al. (2007) New Phyto.
Introduction Past studies Current researches Future prospects
Silene ciliata Pourret• Floral asynchrony• Local adaptation, reciprocal transplants
Intermediate population
Low population
High population
Gimenez-Benavides et al. (2007) Ann. Bot.
Introduction Past studies Current researches Future prospects
Silene ciliata Pourret• Floral asynchrony• Local adaptation, drought resistance
Intermediate population
Low population
High population
Fv/
Fm p
htos
ynte
tic
stat
us
Days without water supply
García-Fernández et al. (2012) Plant Bio.
Low pop.Inter. pop.High pop.
Introduction Past studies Current research Future prospects
Silene ciliata Pourret • Central – marginal hypothesis• Niche model prediction in Central Spain
GuadarramaGredosBéjar
Introduction Past studies Current research Future prospects
Silene ciliata Pourret
• Central population
• Marginal populations
• Central – marginal theory• Niche model prediction in Central Spain• Populations selection, based in:
– 3 mountains– Different population size
Introduction Past studies Current research Future prospects
Silene ciliata Pourret • Central – marginal theory• Niche model prediction in Central Spain• Populations selection• Two complementary approaches:
Ex situ sowing experiment at optimum conditions in growth chamber and greenhouse environment (mother plant x type of cross x block x pop. = 8640 seeds).
In situ sowing experiment at the locations of the 6 marginal populations (mother plant x type of cross x block x pop. = 24000 seeds)
Introduction Past studies Current research Future prospects
Silene ciliata Pourret
Reference-based transcriptome assembly
Silene latifolia Reference Genome
T G T C G G T C TT G T C G G T C T
T G T C A G T C TT G T C A G T C T
SNP calling – Reads2SNP
High
Low
Differential expression
Candidate Genes
High
Low Candidate Genes
Functional annotation&
Enrichment analysis
• Next Generation Sequencing. • Transcriptome analyses and SNP calling.
Introduction Past studies Current research Future prospects
Silene ciliata Pourret
Thanks for your attention
Prof. Jose M. IriondoGroup leader
Javier Morente-LópezPh.D student
Luisa RubioPh.D student
Dr. Alfredo García-Fernández
Dr. Carlos Lara-Romero
RUI AGI CAM SES NAJMOR
F1F2
F3
F1>F2F1<F3 F1=F2,F3 F1=F2,F3F1=F2,F3
Inbreeding depression
No inbreeding depression
F1<F2
Adaptation from MOR
Maladaptation from RUI
In situ
F1<F2,F3F1<F2,F3 F1=F2,F3 F1=F2,F3
Inbreeding depression No inbreeding depression
F1<F2,F3
Inbreeding depression
Ex situ F1=F2,F3
He= 0.63FIS= 0.33Ne= 10
He= 0.75FIS= 0.32Ne= 25
He= 0.66FIS= 0.19Ne= 12
He= 0.70FIS= -0.12Ne= inf
He= 0.70FIS= 0.39Ne= 66
He= 0.51FIS= 0.31Ne= 131
Introduction Past studies Current research Future prospects
Introduction Past studies Current research Future prospects
Silene ciliata Pourret • Next Generation Sequencing. • Transcriptome analyses and SNP calling
– 288 genes expressed differentially