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Result“Breast and lung cancer cell lines with various treatments were tested for this antibody. With regular ECL a band was barely detected at 20 minutes. With super signal pretty clean bands were obtained.” – External Laboratory
Antibody Customer Review: STJ97186 Anti-Histone H2A.X (Phospho-Ser139) Antibody
Antibody Specificity: Antibody Rating:
ProtocolTreatment of materials: 5 Gy radiation.
E.g. Gel electrophoresis: SDS gel electrophoresis.
Transfer: Wet transfer.
Blocking: 3% milk for 30 minutes.
Membrane wash: 3X 5 minutes after primary and secondary antibodies with PBST.
Primary antibody probing: 1:500 dilution at 4 degree Celsius overnight.
Membrane wash: 3X 5minutes after primary and secondary antibodies with PBST.
Secondary antibody probing: 1 hour at room temperature (1:2500 dilution).
Membrane wash: 3X 5minutes after primary and secondary antibodies with PBST.
Visualization: ECL visualization for 5 seconds with super signal.
Cell Line: Breast and Lung cancer cell lines
Method of validation: Western Blot
Primary Antibody: STJ97186 Anti-Histone H2A.X (Phospho-Ser139) Antibody
Secondary Antibody Goat anti-rabbitReference Antibody Beta ActinDilution ratio: 1:500
Gel electrophoresis information: SDS gel Electrophoresis
Transfer information: Wet Transfer.
Lane No. AntigenLoading amount
Primary antibody
Primary antibody
dilution ratio
Secondaryantibody dilution
ratioTarget band KD
Visualization time
1 MDA-MB-468 25ug STJ97186 Anti-Histone
H2A.X (Phospho-Ser139)
Antibody
1:500 1:2500 ~14kDa5 seconds with super
signal5 H1299 18ug
9 DCIS 25ug
The figure provided shows the bands obtained from MDA-MB-468, H1299 and DCIS cells. Only the control cells were indicated in the names because of the confidentiality. The bands appear to be specific. An incresae in the protein was detected in lane 4 and lane 8 as expected.
