Result“I could detect bands using 5 different cell lines with 1:2000 dilution. I could detect the bands as early as 5 seconds exposure.”

“The figure shows the image of the membrane that was blotted for MRE11. 4 cell lines are shown in this blot. Breast cancer cell lines MCF7, MDA-MB-231, DCIS and lung cancer cell line A549. The band was very specific and could be detected at even 5 seconds exposure. The specific treatments are left out because of confidentiality of the project.”– External Laboratory

Antibody Customer Review: STJ94194 Anti-MRE11 Antibody

Antibody Specificity: Antibody Rating:

ProtocolTreatment of materials: 5Gy radiation;

E.g. Gel electrophoresis: SDS gel electrophoresis;

Transfer: Wet transfer

Blocking: 3% milk for 30 minutes;

Primary antibody probing: 1:2000 at 4˚C for overnight;

Membrane wash: 3X 5minutes after primary and secondary antibodies with PBST;

Secondary antibody probing: 1 hour at room temperature (1:2500 dilution)

Membrane wash: 3X 5minutes after primary and secondary antibodies with PBST.Visualization: ECL visualization for 15 seconds (the visualization time was determined by actual result. It was ensured that the visualization time was identical for the same antigen).

Cell Line: Breast cancer and lung cancer cell lines

Method of validation: Western Blot

Primary Antibody: STJ94194 Anti-MRE11 antibody

Secondary Antibody Goat anti-rabbitDilution ratio: 1:2000

Gel electrophoresis information: SDS gel electrophoresis

Transfer information: Wet Transfer

Lane No. AntigenLoading amount

Primary antibody

Primary antibody

dilution ratio

Secondaryantibody dilution

ratioTarget band KD

Visualization time

1 MCF730ug STJ94194

Anti-MRE11 antibody

1:2000 1:2500 ~80kDa 15sec5 MDA-MB-231

9 A54925ug

13 DCIS