CellMissy: enabling management and dissemination of cell migration data

paola masuzzo

paola.masuzzo@ugent.beVIB / Ghent University, Ghent, Belgium

A big challenge in cell migrationis data management and dissemination

sample preparation

automated image acquisition

automated image analysis

A big challenge in cell migrationis data management and dissemination

sample preparation

automated image acquisition

automated image analysis

There are three key elements to software for cell migration data

Guide and capture experimental set-up

Store, structure and query the acquired data

(Semi-)automate data exploration, analysis and interpretation

That’s why we developed CellMissy…

CellMissy modules

Experiment

0 3h 6hwound

cells

Experiment Manager

DataLoader

Data Analyzer

Masuzzo et al., Bioinformatics, 2013

Metadata are crucial

Exchanging the datalab-to-labglobally

Local data management

Metadata are crucial

Exchanging the datalab-to-labglobally

Local data management

Detailed metadata annotationis essential for data interpretation

Detailed metadata annotationis essential for data interpretation

Detailed metadata annotationis essential for data interpretation

Image acquisition metadata also tellan important part of the story

Link to raw images (ordering of imaged wells)

Imaging techniques

Exposure time, light intensity, magnification, time interval

Metadata are crucial

Exchanging the datalab-to-labglobal

Local data management

Structuring the data takes a bit of effort

These data support analysis on multiple levels

These data support analysis on multiple levels

These data support analysis on multiple levels

These data support analysis on multiple levels

These data support analysis on multiple levels

These data support analysis on multiple levels

Metadata and data are capturedon all these different levels

Metadata are crucial

Exchanging the datalab-to-labglobally

Local data management

Metadata and/or data can be easily exchanged between laboratories

lab A

Metadata and/or data can be easily exchanged between laboratories

lab A lab B

Metadata and/or data can be easily exchanged between laboratories

lab A lab B

This is one file in CellMissy! (≈10 MB)

Metadata are crucial

Exchanging the datalab-to-labglobally

Local data management

The real target is a global repositoryfor cell migration data

DataRepository

The real target is a global repositoryfor cell migration data

DataRepository Local Software

http://cellmissy.googlecode.com

Thank you

Niels Hulstaert prof. dr. Lennart

Martens

prof. dr. Leen Van Troysprof. dr. Christophe

Ampe