“DEVELOPMENT OF ENVIRONMENTAL FRIENDLY

NATURAL BIO-PESTICIDE FROM LOCAL PLANT SPECIES

Derris elliptica (TUBA)”

PROJECT COORDINATOR: PROF. DR. MOHAMMAD ROJI SARMIDIPROJECT COORDINATOR: PROF. DR. MOHAMMAD ROJI SARMIDIPROJECT MANAGER/RESEARCHER: SAIFUL IRWAN ZUBAIRIPROJECT MANAGER/RESEARCHER: SAIFUL IRWAN ZUBAIRI

STRATEGIC BUSINESS UNIT – ENVIRONMENTALLY FRIENDLY NATURAL BIO-STRATEGIC BUSINESS UNIT – ENVIRONMENTALLY FRIENDLY NATURAL BIO-PESTICIDE FOR AGRICULTURAL & HORTICULTURAL APPLICATIONSPESTICIDE FOR AGRICULTURAL & HORTICULTURAL APPLICATIONS

PROJECT DESCRIPTION

The main aim of this project is to build a pilot scale processing unit for the processing of rotenone from tuba plant.

The main objective of this project is to develop commercially viable, environmental friendly and human safe bio-pesticide.

Rotenone from Derris elliptica (Tuba) is the main active ingredient (A.i) for bio-pesticide production.

To undertake the potential collaboration project with UPM, USM, MARDI & FELCRA.

LITERATURE REVIEWS

The earliest recorded insecticidal use of rotenone was against leaf eating caterpillars in 1848. Rotenone was isolated 1902.

Rotenone harmless to plants, highly toxic to many insects and relatively innocuous to mammals.

68 species of plants, including: (Jour. Econ. Ento, 1932)

Name of species Number of species

Derris 12

Lonchocarpus 12

Tephrosia 21

Millettia 10

Mundula 13

TOTAL 68

Continue…

Name of plants Rotenone content % (w/w)

Derris elliptica 5 – 9 % ; 31 % *Total Ether Extractives

Derris malaccensis 4 % ; 27 % *Total Extractives

Lonchocarpus utilis 8 – 11 % ; 25 % *Total Extractives

* Toxicarol & deguelin make up the bulk of TOTAL EXTRACTIVES

The content of these materials in a various commercial plants species and varieties is variables:

LITERATURE REVIEWS

Rotenone is a naturally occurring chemical with insecticidal and piscicidal properties extracted from the roots of several plant species belonging to genus Derris spp grown in Malaysia, East Indies and South America or Lonchocarpus spp grown in Central and South America .

Rotenone is being used worldwide to control aphids, maggots, bagworms, diamond back moth, Japanese beetles, leaf hopper and Mexican bean beetles. It is also used to control grubs, ticks, lice and fleas on cattle.

Emulsifiable concentrates and wettable powders of rotenone are also extensively used in lakes, ponds and reservoirs to control undesirable fish.

TUBA SPECIES THAT HAVE BEEN FOUND IN MALAYSIA

TUBA GADING

(Derris malaccensis)

TUBA KAPUR

(Derris elliptica)

TUBA BUAH

(Unknown species)

LOCATION : MARDI JERANGAU, TERENGGANU HERBAL INCULCATION, 2003

BIO-PESTICIDE INDUSTRY SEARCH IN MALAYSIA

Rotenone based bio-pesticide is not available in Malaysia.

No rotenone based pesticide listed in Pesticide Board of Malaysia until 13th April 2004.

There is also no rotenone-based industry listed in Malaysia despite of abundance growth of tuba plants in Malaysian palm oil and rubber plantations. Not to forget that Malaysian forest is home to several tuba species.

Some of the tuba species located in Malaysia produced high rotenone content ex.; Derris spp

Commercial grade bio-pesticide.Efficacy and toxicity data on formulated rotenone bio-pesticide (prototype) for product registration Data for constructing manufacturing scale of rotenone processing plant

Solvent Extraction Process (extraction of rotenone)Evaporation Process (recovery of solvent and concentration of rotenone) Chromatography technique/analysis

MAIN OUTPUTS

TECHNOLOGY FOCUS

QUESTION AND ANSWER SESSION

THANK YOU FOR YOUR KIND ATTENTION

NATURAL BIO-PESTICIDE LABORATORY ,

CEPP, UTM SKUDAI, JOHOR - 2005.