A flexible, affordable and fast R&D outsourcing service partner

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BLIRT (BioLab Innovative Research Technologies) is a private biotechnology company founded in 2008 with head office based in Gdansk, Poland.

BLIRT has a unique hybrid model, an innovative biopharmaceutical service provider with Contract Research Organization. BLIRT offers a suite of biology and chemistry services, attractive especially for discovery and lead optimization development phase.

BLIRT has modern laboratories and competent staff with a high percentage of internationally educated PhDs.

We are able to accommodate small and bigger projects, and can engage project resources on long term contract or as-needed basis.

Company overview

www.blirt.eu

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891 publications in the field of life science

( Medicine, Microbiology, Molecular Biology, Biotechnology, Biochemistry, Organic Chemistry, Analytical Chemistry, Molecular Modeling)

Research Team

BLIRT employes 34 people after 2 years activity.

The Research Team consists of 22 researchers where 9 are PhDs at molecular biology, biotechnology, organic chemistry and computer chemistry.

www.blirt.eu

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Our machinery

Protein

CRO & CMO

Analysis and

Bioanalysis

Organic Synthesi

s

www.blirt.eu

We offer a broad spectrum of custom services from gene to protein helping you to speed up your development and to increase the efficiency.

Gene Production

Purification Protein

In silico gene synthesis

Metagenomics

PCR

DNA and cDNA library construction

Gene engineering

Proof of concept

Difficult to express proteins solutions

E.coli, P.pastoris, B. megaterium,

S.cerevisiae

Flask and Bioreactor (10L)

expression optimizations

Downstream Processing Optimization

Chromatography methods

development and validation

Unique resin synthesis for special proteins

Formulation Adjustment

Analytical testing

Protein Department

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Choosing the right expression strain of E.coli

1 2 3 4 5 6 M 7 8 9 10 11 12

Expression T7 system: OD600=0,3, IPTG to 1mM, 30oC, 6h, TB broth.

1. E.coli strain 1 protein I (whole fraction)2. E.coli strain 1 protein I (soluble fraction)3. E.coli strain 2 protein I (whole fraction)4. E.coli strain 2 protein I (soluble fraction)5. E.coli strain 3 protein I (whole fraction)6. E.coli strain 3 protein I (soluble fraction)M. Protein weight marker (170, 130, 100,

70, 55, 40, 35, 25, 15, 10 kDa)7. E.coli strain 4 protein I (whole fraction)8. E.coli strain 4 protein I (soluble fraction)9. E.coli strain 5 protein I (whole fraction)10. E.coli strain 5 protein I (soluble fraction)11. E.coli strain 6 protein I (whole fraction)12. E.coli strain 6 protein I (soluble fraction)

Protein Department

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Screening for expression temperature M 1 2 3 4 5 6

Expression T7 system: OD600=0,3, IPTG to 1mM, various oC, 6 h, TB broth.

M. Protein weight marker (170, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa)1. 18oC E.coli strain 1 protein II (whole fraction)2. 18oC E.coli strain 1 protein II (soluble fraction)3. 30oC E.coli strain 1 protein II (whole fraction)4. 30oC E.coli strain 1 protein II (soluble fraction)5. 37oC E.coli strain 1 protein II (whole fraction)6. 37oC E.coli strain 1 protein II (soluble fraction)

Purification M 1 2 3 4 5

Expression T7 system: OD600=0,3, IPTG to 1mM, 37 oC, 6 h, TB broth.

M. Protein weight marker (130, 90, 66, 40, 25, 10 kDa)1. E.coli strain 2 protein III (soluble fraction)2. E.coli strain 2 protein III DEAE Sepharose week anion

exchanger3. E.coli strain 2 protein III Mono Q strong anion exchanger4. E.coli strain 2 protein III Octyl Sepharose HIC5. Formulated protein III

Protein Department

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DEAE Sepharose1. pH=4,52. pH=5,5M. Protein weight marker (170, 130,

100, 70, 55, 40, 35, 25, 15, 10 kDa)4. pH=6,55. pH=7,56. pH=8,57. pH=9,58. pH=10,5CM Sepharose9. pH=4,510. pH=5,511. pH=6,512. pH=7,513. pH=8,514. pH=9,515. pH=10,5

1 2 M 4 5 6 7 8 9 10 11 12 13 14 15

Loading buffer containing L-Arginine as additive at various pH.Elution stepwise with 50mM Hepes 1M NaCl pH=7,0

Loading buffer containing Triton X-100 (R) as additive at various pH.Elution stepwise with 50mM Hepes 1M NaCl pH=7,0

Loading buffer at various pH.Elution stepwise with 50mM Hepes 1M NaCl pH=7,0

Intracellular production of a therapeutic protein by E.coli.Soluble protein

Protein Department

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BLIRT specializes in development of new methods and analyses that are not possible in diagnostics laboratories. We are also able to conduct all routine tests, particularly those that require our unique equipment.  

Example analyses that we are able to perform:

• Analysis of drugs and its metabolites concentration in blood, urine, other biological fluids and tissues,• Pharmacokinetic analysis after drug administration,• ADME studies – complex analysis of absorption, distribution, metabolism and excretion,• Analysis of purity, stability and protein binding of pharmaceuticals, • Analysis of biochemical markers in blood, urine, other biological fluids and tissues,• Determination of molecular weight and purity of proteins and peptides,• Protein and peptide sequencing following partial proteolysis.     

Analysis department

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BLIRT can provide you access to most advanced analytical equipment and high profile scientists with many years of experience in biomedical analytics.   

Overview of Analytical Methods: Ultra-High Performance Lipid Chromatography with mass detection (UHPLC-MS/MS) connected to newest generation of quadrupole time of flight mass detector (Q-TOF) with source of ions type electro-spray (ESI) or chemical ionization under atmospheric pressure (APCI).High Performance Lipid Chromatography (HPLC) with detection: RID, FL and UV-DAD.Infrared spectroscopy with Fourier Transformation (IR).Spectroscopy UV-VIS. 

Overview of Analytical Equipment:Spectrometer UV-VIS – JascoSpectrometer IR- JascoHPLC-MerckUHPLC-DionexPLC- DionexGC-Perkin- Elmer

Analysis department

Detectors: micrOTOF-Q-II - BrukerRID – ShodexFL-MerckUV-DAD - DionexFID- Perkin ElmerECD-Perkin ElmerNPD- Perkin Elmer MS/MS - Bruker

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BLIRT – Synthesis services We offer all-embracing organic synthesis services directed to industrial and scientific customers:

Pharmaceutical Industry; Chemical Industry; Academic Scientists;

In particular we specialize in synthesis: chiral compounds with natural origin – with synthetic and bio-catalytic methods; chiral unnatural/non-peptidic amino acids; peptides (‘on solid state’ and ‘in solution’); heterocyclic compounds (with N, O, S in ring structure); macrocyclic compounds; phosphoroorganic compounds;

Synthesis department

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Custom synthesis - comprehensive equiped laboratory: mg – 100s g scale;

Design of alternative synthetic pathways;

Optimalization of synthetic routes;

Reaction yields optimalization;

Identification, synthesis and standarization of API impurities;

Identification, synthesis and standarization of drug metabolites; CHO

F CH3

COOMe

BrCOOMe

CH3

O

FCOOMe

CH3

OH

F

COOH

CH3

OH

FO COOH

CH3O CN

CH3

A B

C

DE

We offer comprehensive organic synthesis and interdisciplinary chemistry services:

Synthesis department

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Fee for Service

Co-development

FTE

Consulting

Collaboration models

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Key benefits of collaboration• Competitive pricing• Short project deadlines• Strong scientific team• State of the art facilities

Service Quality• ISO 9001:2009 - certificate• GLP - pending.- 03.2011• GMP – planned for 2013

Summary