Citrus-A case study

Chinju Maria Mathew008315

Citrus…???» No 1 : Nutritional value and productivity.» Origin : 4000 BC in South-East Asia.» Family : Rutaceae (Evergreen Trees)» Nature : Acidic.» Reservoir : Vitamin C, Vitamin B9 and

Dietary Fibers.» Phytochemicals : Reduces risk of many

Chronic diseases.

Millennium Break Through !!!

» 4 decades of research – Poor Practical Results.» Micro grafting.» Nucellar Embryogenesis.» Shoot Meristem Regeneration.

My pick…“ Tissue Culture of Citrus Cultivars ”

At a glance…» Nuclear Institute for Agriculture and Biology.» Kinnow-Mandarin, rough Lemon, Sweet Lime,

Gada-dehi, Washington Navel, Grapefruit.» Aims :

Production of healthy and vigorous calli.Regeneration from calli.Production of Somatic Hybrids.

In Lab…» Explants :

Nucellus, Under developed seeds, Seedling leaf, Basal node and Ovary.

» Cultured on MS medium, supplemented with

BA + Kn + 2,4-D + NAA + IAA + (Glutamine/Malt extract)

» Reciprocal Crosses.» Seed Characteristics.

To the world…?» Success lies on Explant and Medium.» Only 2,4-D induced Callusing.» BA + 2,4-D => Good Quality Embryogenic Calli.» BA + Kn => Shoot and Root Induction.» 2,4-D + Kn => High Quality Calli.» Callus formation on the 10th day – Creamy White.» Sub-culturing turned GREEN and into mature

embryo.

To the world…?(Cntd)» Maturation directly proportional to Dryness of

the Medium.» Secondary Embryogenesis and Pseudo bulbils

formation.» Production of Somatic hybrids.» Tendency to fruit drop (Kinnow).» Gada-dehi, maximum number of healthy seeds.

My point of view…POSITIVES Explant - Nucellus. Detailed study of plant growth hormones on

Explant Detailed study of seed characteristics. Production of Somatic Hybrids. Formation of Pseudo bulbils and Secondary

Embryogenesis.

My point of view…(Contd.)NEGATIVES Nucellus exhibit the phenomenon of Juvenility

and delayed fruiting. Higher concentration of 2,4-D => Browning of

medium. Explant collected from field grown trees

induced Contamination. Notable difference and its explanation in

weight of seeds is missing. Tendency to fruit drop in hybrid plants.

To sum it up…» Tissue culture and micro propagation – an

integral part of plant transformation.» Production of Citrus increased steadily.» Research was indeed successful and they came

up with variety of explants.» Resulted in the propagation of healthy citrus

plants.

References.

Thnkz….