Differential diagnosis of brucellosis serologic reactions

Differential Diagnosis of Brucellosis Serologic Reactions

Steven G. HennagerBacterial and Hemoparasitic Serology Team

National Veterinary Services Laboratories

Workshop: An Integrated Approach to Controlling Brucellosis in Africa, Addis Ababa, 29-31 January 2013

Safeguarding Animal Health

Dominant Brucella Antigens

Species • Brucella melitensis• Brucella abortus• Brucella suis• Brucella canis• Brucella ovis

Primary Antigen • M and A• A and M• A and M• Rough• Rough

Blue colonies = Smooth Brucella sp.Crystal colonies = Rough Brucella sp.


Immune response to Brucella infection

• Cell mediated response

• Humoral or antibody production


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Serologic Assays Antibody detection

Serum Milk

Rapid Inexpensive Sensitivity and Specificity Assays can be impacted by

Vaccination Environmental conditions

Brucella card test

Brucellosis Diagnostic Tests







Other Brucella Diagnostic Tests

• Polymerase Chain Reaction (PCR)• Culture Identification• Western Blot

Amount of Isotype (ng)

Test IgM IgG1 IgG2 IgA

Tube 20 ----- 125 650

Card ----- 600 7500 -----

Riv ----- 1550 2750 -----

CF 6500 290 ----- -----

ELISA 155 190 220 700

Brief History 19th century – Bruce isolated organism

from human spleens Linked organism to raw goat’s milk Genus named after him

19th century – Bang discovered organism that caused abortions in cattle Named organism Bacillus abortus

20th century – Evans recognized the relationship between Bruce’s organism and Bang’s organism Advocated pasteurization of milk Re-named organism Brucella abortus

Sir David Bruce

L.F. Bernhard Bang

Alice C. Evans

Acknowledge Superior Laboratory Support

• De Anne Wall, Diane Davis, Barb Kesl, Molly Fritz– Serology Laboratory Technicians

Questions?

Technology

Differential diagnosis of brucellosis serologic reactions