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Will you know next week – or in fifteen years?
EverTrieve™
Catalogued, tamper-proof storage of large data files
Do you know where your flow data are?
Do you know what was in your experiment?
with Cytogenie™
in a self-managing on-line/off-line archive
Information flow in a typical biomedical study
Publications, Patents
Study
Questions, Hypothesis
Experiments
Answers, Interpretations
Instrument info
Annotated Raw Data
Sample info
Annotated Sample Data
Subject info
AnnotatedSubject Data
Statistical Analysis
Experiment info
Annotated Tube Data
Tube and reagent info
Measurement instrument e.g., FACS
Reagent sets
A B A B
Samples from#1, 2
Tubes U U U U
Subjects #1, 2… W
here do I s
tart?
FacsXpertCytoGenie
1. Start at the beginning
Plan your experiment with CytoGenie
• Let CytoGenie do all your computationsFacsXpertCytoGenie
• Specify your dilutions and steps and the controls you want
Record all the information you need.
• Pick the best reagents for your instrument set-up
•Create keywords for tables & labels for subjects and samples
Correctly. And only once.
• Build the best stain sets for single and multi-step staining
•Print a full pipetting guide for technicians and your lab
notebook
FACS Curator
2. Keep EverTrieve’s
FacsXpert
Your instrument computer
FACSCurator
on your instrument computer(s)
Keywords
Labels
FACS Curator
labels and keywords
Instrument settings
FacsXpert
Your instrument computer
DiVa
FACSCurator
to DiVa*
passes instrument settings
Instrument
settings
Labels
Keywords
FacsXpertCytoGenie
Your experiment protocol
*Integration with other
instrument software on demand
FACS Curatorbundles collected datawith your protocol in a
tamper-proof wrapper
collected data+ protocol
collected data protocol
Tamper-proofwrapper
Eve
rTrie
ve
3. Keep EverTrieve
on its own server
tamp
er-p
roo
fw
rapp
er
Eve
rTrie
ve
FacsXpert
Your instrument computer
FACS
Curator
FACS Curator
passes the tamper-proof wrapper
to the EverTrieve server
Eve
rTrie
ve
EverTrieve
catalogs the content
of each bundle of files,
including experiment descriptions,
instrument settings, reagents,
keywords, subjects, samples,
and other metadata from CytoGenie
Eve
rTrie
ve
EverTrieve
emails the catalog
with a link to the data
to the researcher and
anyone he or she designates
Researcherscan search the EverTrieve catalog
in the email or on a password-protected website
Will you know next week – or in fifteen years?
EverTrieve™
Do you know where your flow data are?
Do you know what was in your experiment?
with Cytogenie™
EverTrieve has been running for over three years in theStanford Shared FACS Facility, serving over 400
users and collecting 10GB of flow data from eight different instruments every week.
It has not lost a single data bit.
