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MAPPING OF WHISKER SENSORY INPUT IN THE TRIGEMINAL GANGLION OF THE
MOUSE
Stephanie RedmondDavid W. MatthewsDr. David Kleinfeld
University of California, San Diego
Mapping the Trigeminal Ganglion
• Where is the trigeminal ganglion (TG)?• What makes up the TG?• Whisker follicle injections• Image analysis• TG patterning• Going forward
Adapted from Kleinfeld, et al. 2008
Where is the TG?
Adapted from Kleinfeld, et al. 2006
What makes up the TG?
• Many different cell types– Whisker sensory neurons– Temperature-sensing neurons– Motor (movement) neurons
• Functions– Correlations to vision
• Hyperacuity– Trigeminal neuralgia
• Blood vessels rub against TG cells
R
M
L
C
rostral (R), caudal (C), lateral (L), medial (M)
Why should spatial organization exist?
TG Studies:• Anatomy • Gene Expression
Adapted from Waite 1992 Adapted from Hodge 2007
Whisker Follicle Injections
• Fluorescent retrograde neuron labeling– Alexa Fluor® Cholera Toxin Subunit B– Wild type mice, age ~P16
• TG histological slice– 50 to 75 micrometer slices
488 nm
555 nm
647 nm
Image Analysis
• Confocal Microscopy• Image through sample (~50-100 um)• Develop image stacks
"Confocal Microscopy", D Semwogerere & ER Weeks, published in the Encyclopedia of Biomaterials and Biomedical Engineering, Taylor & Francis (2005).
Screen with pinhole
Focal Point
Sample EA (488) B (555) C (647)
R
M
L
C
Image Analysis• Spatial Organization
• Measure cell height, width, length• Calculate and compare average:
• Position, volume
A (488) B (555) C (647)
R
M
L
C
TG Patterning
_x0001_A _x0001_B _x0001_C _x0001_D _x0001_E0
200
400
600
800
1000
1200
Average Cell Cross-sectional Area
12345
Whisker
Squa
re M
icrom
eter
s
Going Forward• Process image data
• Investigate other patterns• Average X-coordinate• Average Y-coordinate• Average volume
• Finish thesis paper
• Graduate
Acknowledgments
David W. MatthewsDr. David KleinfeldThe Kleinfeld LabPhysics REU ProgramUCSD
MPS FacultyProfessor ShilepskyProfessor Heinekamp
MPS MajorsWells College
