Polymerase Chain Reaction

By: Stephen Feagles

What is PCR?the automated process that allows a specific

DNA chain also called primers to be replicated over and over again to produce more copies of that DNA

Who Invented it? Dr. Kary Banks Mullisborn on December 28, 1944Ph.D. degree in biochemistry from the

University of California, BerkeleyWon Nobel Prize in Chemistry for PCR

Thermocycler This machines purpose is to alter the

temperature so that the primers can be heated and cooled to perform the processes correctly

Steps of PCR1. Denature 2. Annealing 3. Extension

DenatureHeating up DNA Splitting into two strands 94 degrees Celsius

Annealing Cooling DownAllowing to be bonded 54 degrees Celsius

Extension Taq Enzyme creating copy of original DNATwo new double stranded DNA formed 72 degrees Celsius

Process of PCR (video) http://www.youtube.com/watch?v=eEcy9k_Ks

DI

PCR Importance Creates more DNA

More tests run on specific DNAMore DNA can be tested by different people

ForensicsAllows small amount of DNA to be made into a

lot Research

Mutated DNA can be reproduced and be tested different ways

Bibliography http://vadlo.com/Research_Cartoons/http://

www.youtube.com/watch?v=eEcy9k_KsDIhttp://

flickrhivemind.net/Tags/lab,pcr/Interestinghttp://

www.sivb.org/InVitroReport/IVRs/38-1/karyMullis.asp

http://www.btci.org/k12/bft/PCR/pcr_geneticscreening.html