Embed Size (px)
DESCRIPTION
This is a short presentation that shows the working mechanism and the applications of the new ionization source, for mass spectrometry, named Surface Activated Chemical Ionization (SACI). More informations can be found at the URL: http://www.isbiotechnology.com
Citation preview
1
Surface Activated Chemical Surface Activated Chemical Ionization – applications to low Ionization – applications to low molecular weight drugs molecular weight drugs analysisanalysis
Simone CristoniSimone Cristoni
2
Surface Activated Chemical Ionization
3
Surface Activated Chemical Ionization
Controlled, low
potential
Metal surface
4
SACI
High sensitivity
High Versatility (different eluent flow rates, compounds and matrix)
Cost effective (avoid pre purification step working by sample dilution)
5
Analysis of addictive drugs
Urine
Hair
Saliva
Sample dilution 1:10
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
6
Analysis of addictive drugs
Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) Acquisition mode: tandem mass spectrometry (MS/MS) Chromatography column: C18 50x4.1 1.8 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA Eluent flow: 500 µL/min Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes,
from 40 to 5 % in 2 minutes. Sample volume injected: 20 µL
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Experimental conditions
7
Analysis of addictive drugsUrine 2 ng/mL
APCI
SACI
8
Analysis of addictive drugsSaliva
4 Retention time (min)
14
a) SACI
Sig
nal
in
ten
sity HCT
0
5.3E+5 12.40
LTQ
4 Retention time (min)
14
a) SACI
Sig
nal
in
ten
sity HCT
0
4.1E+4
LTQ
40
Sig
nal
in
ten
sity
3.2E+2
Retention time (min)
14
b) APCI
HCT
LTQ
Sig
nal
in
ten
sity
0
1.89E+2
Retention time (min)
14
c) ESI
4
HCT
LTQ
0
Sig
nal
in
ten
sity
5.6E+2
Retention time (min)
14
b) APCI
4
HCT LTQ
Sig
nal
in
ten
sity
4.2E+3
Retention time (min)
14
c) ESI
04
LTQHCT 12.20
12.30
10 ng/mL of 6-MAM 1 ng/mL of 6-MAM
9
Analysis of pesticides
Water Spinach Sample dilution 1:10
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Different matrix
10
Analysis of pesticides
Mass spectrometer: ESI-TOF (Bruker Daltonics) Acquisition mode:High mass accuracy full scan MS Chromatography column: C18 50x4.1 3,5 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3OH + 0.1% FA Eluent flow: 500 µL/min Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes,
from 40 to 5 % in 2 minutes. Sample volume injected: 20 µL
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Experimental conditions
11
Analysis of pesticideswater
12
Analysis of pesticidesspinach
13
Analysis of toxin in food
Wine Grape Sample dilution 1:10
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Different matrix
14
Analysis of pesticides
Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) Acquisition mode: tandem mass spectrometry (MS3) Chromatography column: C18 250x4 5 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA Eluent flow: 1mL/min. Gradient: %B: 5 for 5 minutes, from 5 to 70 % in 10 minutes,
from 70 to 5 % in 2 minutes. Sample volume injected: 20 µL
Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow
gradient chromatography
Experimental conditions
15
Analysis of pesticidesspinach
16
Analysis of OTAWine – 10 ng/mL
17
Conclusions
High sensitivity (LOD, LOQ, linerity ranges) Cost effecting avoiding matrix effect and pre-concentration step
(e.g. Solid Phase Extraction) Possible to use it in a wide range of chromatographic conditions. High throughput screening of complex mixture if coupled with
high mass accuracy analyzers (e.g TOF or FTICT).
18
Future developments
Ion exchange chromatography (low operative voltages employed in SACI makes possible to operate in these chromatographic conditions).
High sensitive quantitation analysis of peptide and protein drugs (ioni monocarica; TOF).
Pesticides (developing high sensitive methods for screening of selected groups of pesticides of interest)
19
SACI demo site
Web site: http://www.isbiotechnology.com
20
acknowledgment
Dr.ssa Sara Crotti – ISB Milan Dr. Lorenzo Zingaro – ISB Milan Dr.ssa Cristina Canton – ISB Milan Dr.ssa Laura Molin – ISB Milan Dr. Pietro Traldi – CNR Padova Prof. Luigi Rossi Bernardi – University of Milan Dr. Simone Rubini – Bruker Daltonics Dr. Elisa Basso – Bruker Daltonics Dr. Leopoldo Dimiziano – Bruker Daltonics
21
www.bdal.com
