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Transconjugants (pSymA + pSymB). 100 bp. 1021RctAHB. AtGMI9023. 200 bp. M100bp. M100bp. 1. 2. 3. 4. 5. 6. 7. 8. SMb21314 (wgeA). SMb20946 (exoY). SMb20094. SMa1077 (nex18). SMa4202 ( rhbB). - PowerPoint PPT Presentation
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100 bp
200 bpSMb21314 (wgeA)
SMb20946 (exoY)
SMb20094
SMa1077 (nex18)
SMa4202 (rhbB)
M10
0bp
AtG
MI9
023
1 2 3 4 5 6 7 8 M10
0bp
1021
Rct
AH
B
Transconjugants (pSymA + pSymB)
Figure S2. PCR amplication of genes from pSymA and pSymB in A. tumefaciens GMI9023 transconjugants that received both plasmids simultaneously. The recipient strain AtGMI9023 was PCR-negative for all marker genes, and the donor strain 1021RctAHB was used as a positive control. Note that transconjugants 1 and 2 only amplified SMa1077 and SMb20094 genes, whereas the remaining transconjugants examined gave positive amplification of all 5 genes tested. M100bp, DNA molecular weight marker.
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