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WHEN NOT HING ELSE MAT T ERS MORE T HAN ACCURAT E RESULTS
In the ever changing world of Biopharmaceuticals, reliable and robust separation technologies are
needed to keep pace with this fast growing area of discovery. System accuracy and reproducibility
mean everything to scientists who stake their reputations on the results they generate or methods
they develop.
The Alliance® HPLC Bioseparations System, a high-precision, high-resolution HPLC system for the
fractionation and analysis of complex biomolecule mixtures, is ideally suited to help accelerate the
progress of your biotechnology analyses.
The core of the Alliance HPLC Bio System is the Waters e2796 Bioseparations Module. Designed
for robust and reliable operation under the harsh salt and pH conditions typical of bioseparations,
it features an inert flow path, making it ideal for protein purification, peptide mapping, and nucleic
acid analysis.
ALLIANCE HPLC BIOSEPARATIONS SyST EM
THE ALLIANCE HPLC BIOSEPARATION SySTEM fEATURES:
n Biocompatible flow path (Titanium/PEEK)
n Auto•Blend™Technology,whichblendsuptofoureluentstoperformisocratic,step,continuousgradient,orflowprogramapplications
n AutomatedSystemPREProutineforrapidunattendedsystemequilibration
n Precisetemperaturecontrolforenhancedsamplestability
n Compatibilitywithhigh-pressureandlow-pressurecolumnchemistries
n Automatedcolumn switching,flowdiversion,andfractionmanagementtosupportcomprehensivemulti-dimensional separationsmethods
n Enhancedsystem monitoringfunctions (pH,conductivity, composition,valve, andfractiontracking) formethodsdevelopment andtroubleshooting
n Provenday-to-dayreproducibility
The Alliance HPLC Bioseparations System may be configured to provide fully automated purification platforms for proteins, peptides, and other biomolecules.
High sensitivity UV/Visible
or PDA detection allows for
the simultaneous quantitation
of both major and minor
components.
Samples can be processed from
standard 96-, shallow- and deep-
well plates, 384-well microtiter
plates and custom plates,
2-mL vials, or 0.5- and 1.5-mL
microcentrifuge tubes.
Available online pH and
conductivity monitors enable you
to optimize complex separations
using real time buffer composition
information.
Sample temperature control
enhances biomolecule stability
prior to analysis.
Titanium flow path is ideal for
biomolecule separations using
all modern bioseparation
chemistries.
Switching
valves expand
the system’s
capability
to include
higher peak
capacity, multi-
dimensional
separations.
BioSuite™ HPLC Columns are
high performance chemistries
developed specifically for the
isolation, analysis, and charac-
terization of proteins, peptides,
and other biomolecules.
Quaternary eluent blending
enables the automatic
formulation of buffers from
simple stock solutions
using Auto•Blend methods.
Solventmanagement
The simplified design of the e2796 Solvent Manager enables the
blending and delivering of solvents in a pulse-free, low-dispersion
flow path.
In addition, Synchronized Composition Control™ (SCC™) of the gra-
dient proportioning valve (GPV) ensures that the e2796 Separations
Module delivers excellent gradient composition accuracy and precision.
The overall design results in reduced complexity and enhanced overall
ruggedness across a flow range that covers two orders of magnitude.
Automatedsystemstart-up
The Alliance HPLC Bio System also features SystemPREP – a
programmable, automated system preparation routine – for fast,
one-touch, walk-up operation. This function performs a series of
automated steps, putting the separations module in a condition of
complete system readiness for running samples. SystemPREP is
useful when you are changing solvents or after extended periods of
system inactivity and is a simple-to-use alternative to running the
individual functions of Wet Prime, Purge Injector, Equilibrate, and
Condition Column.
INT EGRAT ED fLUIDICS fOR CONSIST ENT PERfORMANC E AND ENHANC ED REP RODUCIBIL IT y
The Waters e2796 Bioseparations Module is the core of the system. Its integrated solvent management and sample management provides
consistent performance and enhanced reproducibility for high quality, high-throughput analysis for HPLC, LC/MS, or flow injection-mass
spectrometry applications.
5%
90%
5%
0%
A
B
C
D
Water
Acetonitrile
100 mMAmmonium
Formate
100 mMAmmoniumBicarbonate
Initial Conditions final Conditions
Initial final
90%
5%
5%
0%
5 mMbuffer
5 mMbuffer
Samplemanagement
The design of the e2796 Sample Manager ensures precise sampling
– delivering predictable peak areas, and consistent results.
Complimenting the simplified design of the solvent flow path, a user
programmable needle wash, and selectable “purge-wash-purge”
cycle ensures that any part of the system exposed to sample is
fully washed, virtually eliminating the risk of sample carryover.
The inherent versatility of the three-axis (X, Y, Z) needle assembly
of the e2796 Sample Manager allows for greater applications
flexibility. The sample manager has a wide range of sample loops,
holding loops, and syringe sizes available and can process samples
from a variety of plates, vials, and/or tubes. This variety allows
you to customize the configuration of the sample manager according
to the needs of your applications.
Auto•BlendTechnology
The e2796 Separations Module features Auto•Blend Technology,
which enables you to generate gradients automatically without
having to manually alter the buffer concentration. Auto•Blend
programming technology improves uptime since no premixing of
mobile phases is required.
Independently driven pistons achieve smooth solvent flow – without pulse dampeners – reducing gradient volume
Dual pressure transducers enable true pulse-free solvent flow High-efficiency
vacuum degassing technology
Precise solvent blending delivers highly reproducible gradients
Tool-free routine maintenance on plungers/seals/seal-wash seals
Simplified flow path with only two inlet check valves
BE ASSURED. CHOOSE WATERS GLOBAL SERVICES.
With94officesinmorethan50countries,onlytheWatersGlobalServicesteamhasthemostin-depthandup-to-dateknowledgeofthe
advancedscienceandtechnologiesthatprovidethefoundationforAllianceHPLCSystems.Maximizesystemuptime,increaselaboratory
productivity,andmeetstringentcompliancerequirementswiththeexpertiseandresponsivenessyouneed.
DO MORE WIT H yOUR RESULTS
Waters software solutions enable you to capitalize on the results generated by your system. The Waters Laboratory Informatics
Suite includes proven information management solutions that integrate all your lab resources in a consistent, scalable, and
seamless application environment.
LEADING BIOSEPARAT IONS CONSUMABLES
When your work involves the analysis of proteins, peptides, and amino acids, Waters has a variety
of chemistry consumable solutions for biomolecule separations in areas ranging from proteomics
to the validation of well-characterized biopharmaceuticals.
BioSuiteHPLCColumns
Designed specifically for bioseparations, Waters’ line of BioSuite
HPLC Columns deliver the resolution and recovery of proteins and
peptides scientists need. Available in ion exchange, size exclusion,
hydrophobic interaction, and reversed-phase modes, BioSuite HPLC
Columns are available in analytical as well as “lab scale” prepara-
tive dimensions.
Peptideseparationtechnologycolumns
Peptide Separation Technology is a unique column chemistry that
meets the demanding requirements of peptide separations used in
a range of applications. Available in 130 Å and 300 Å pore sizes,
Peptide Separation Technology columns are available in numerous
particle sizes and column dimensions for consistent separations,
from high-sensitivity proteomics, to peptide mapping, to high mass
load purification. Peptide Separation Technology columns are
specifically QC tested with a protein digest to ensure exceptional
application performance.
Waters BioSuite HPLC Columns.
Empower™ Software provides control
of the system, including data acquisition,
management, processing, and reporting, all
in the market-leading chromatography data
software (CDS) platform. Empower Software
is scalable from a single workstation to an
enterprise-wide CDS solution, and offers
advanced features for method development,
reporting, and custom calculations, as well
as Enterprise Options for advanced data
management and automated method valida-
tion management.
MassLynx™ Software, for advanced
mass spectrometry applications, enables
researchers to acquire, analyze, manage,
and distribute information. MassLynx
Software offers flexible control over complex
instrument configurations, and Application
Managers that are tailored for specific MS
analyses.
NuGenesis® SDMS is a customizable scien-
tific information management platform that
automatically captures and stores data in
a centralized data warehouse. SDMS makes
information easier to access, facilitates
regulatory compliance, and aids in the
administration of intellectual property. SDMS
converts scientific results into accessible and
valuable corporate knowledge.
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WatersCorporation 34 Maple StreetMilford, MA 01757 U.S.A.T: 508 478 2000F: 508 872 1990www.waters.com
Waters, Alliance, and NuGenesis are registered trademarks of Waters Corporation. The Science of What’s Possible, Auto•Blend, BioSuite, Synchronized Composition Control, SCC, Empower, and MassLynx are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.
©2008 Waters Corporation. Produced in the U.S.A.November 2008 720000589EN LB-PDF
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