AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide

Tube 1 (substrate) Tube 2 (enzyme)H2O2

GuaiacolDistilled H2O

Peroxidase Distilled water

+ Mix contentsBegin Timing

Tube 3(tubes 1 + 2)Begin Timing

AP Lab Skills Guide

Tube 3(tubes 1 + 2)Begin Timing

Each minute for 5 min compare the color of tube 3 to the standard

Time (min) Color

1 1

2 2

3 4…Graph the data…

AP Lab Skills Guide

Each minute for 5 min compare the color of tube 3 to the standard

Time (min) Color

1 1

2 2

3 2

4 3

5 5

6 6

7 8

8 10

You are determining how long to let the reaction go to get a value of “5” as your baseline for comparisons in the next step…you will see…we will run all future reactions for 5 minutes and look at color and compare to enzyme in distilled water….

AP Lab Skills Guide

AP Lab Skills Guide

Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6PeroxidaseH2O2

pH 2 buffer

PeroxidaseH2O2

pH 4 buffer

PeroxidaseH2O2

pH 6 buffer

PeroxidaseH2O2

pH 8 buffer

PeroxidaseH2O2

pH 10 buffer

PeroxidaseH2O2

pH 12 buffer

Cover with parafilm, gently mix and measure color at 0 minutes and color change at time determined in part 1 (5 minutes)...

AP Lab Skills Guide

pH Color

2 1

4 2

6 5

8 3

10 1

12 1

Of course, we should combine the class data, take averages, SD, SE and graph with error bars…

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide

AP Lab Skills Guide