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AP Lab Skills Guide. AP Lab Skills Guide. AP Lab Skills Guide. +. Mix contents Begin Timing. Tube 1 (substrate) Tube 2 (enzyme). Tube 3 (tubes 1 + 2) Begin Timing. H 2 O 2 Guaiacol Distilled H 2 O. Peroxidase Distilled water . AP Lab Skills Guide. - PowerPoint PPT Presentation
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AP Lab Skills Guide
AP Lab Skills Guide
AP Lab Skills Guide
Tube 1 (substrate) Tube 2 (enzyme)H2O2
GuaiacolDistilled H2O
Peroxidase Distilled water
+ Mix contentsBegin Timing
Tube 3(tubes 1 + 2)Begin Timing
AP Lab Skills Guide
Tube 3(tubes 1 + 2)Begin Timing
Each minute for 5 min compare the color of tube 3 to the standard
Time (min) Color
1 1
2 2
3 4…Graph the data…
AP Lab Skills Guide
Each minute for 5 min compare the color of tube 3 to the standard
Time (min) Color
1 1
2 2
3 2
4 3
5 5
6 6
7 8
8 10
You are determining how long to let the reaction go to get a value of “5” as your baseline for comparisons in the next step…you will see…we will run all future reactions for 5 minutes and look at color and compare to enzyme in distilled water….
AP Lab Skills Guide
AP Lab Skills Guide
Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6PeroxidaseH2O2
pH 2 buffer
PeroxidaseH2O2
pH 4 buffer
PeroxidaseH2O2
pH 6 buffer
PeroxidaseH2O2
pH 8 buffer
PeroxidaseH2O2
pH 10 buffer
PeroxidaseH2O2
pH 12 buffer
Cover with parafilm, gently mix and measure color at 0 minutes and color change at time determined in part 1 (5 minutes)...
AP Lab Skills Guide
pH Color
2 1
4 2
6 5
8 3
10 1
12 1
Of course, we should combine the class data, take averages, SD, SE and graph with error bars…
AP Lab Skills Guide
AP Lab Skills Guide
AP Lab Skills Guide
AP Lab Skills Guide
AP Lab Skills Guide
