Biosensors Final Presentation - Fall 2015

Photonic Biosensors

Faculty AdvisorDr. Seung Kim

© Israel Flores 2016

Importance

• Hundreds of thousands of deaths from treatable diseases in each year• Early detection increases survival rates

© Israel Flores 2016

Our Goal

• Early diagnosis the key to effectively

treating diseases like cancer.

• Low Cost

• Point of Care Diagnosis

• Portable Biosensor

To develop a Point-of-Care (POC) Biosensor for medical diagnosis to monitor health status

© Israel Flores 2016

CD Based Microfluidics

Laura DeOtteLuke Funk

© Israel Flores 2016

Why CD-based Microfluidics?•Optical system requires fluid to be in a

~2.5 µm cavity

•Microfluidics effectively transports fluid from input to sensing region

• CD-based design: • Space effective

• Cost effective

• Controllable fluid flow

© Israel Flores 2016

Microfabrication: Photolithography

• Silver sputtering

• SU8 spin deposition

• UV light exposure

• SU8 developing

• Bonding

© Israel Flores 2016

Fall 2015 Design

© Israel Flores 2016

CD Mask Design

© Israel Flores 2016

Channel Selection

• Fluid ‘split’ in channels with a large change in cross-section

• Fluid continuous in uniform channels • Easier to fabricate • 1 mm channel width

© Israel Flores 2016

Results

• Capillary action capable of filling channel and sensing region

• Reduce fill time by spinning

© Israel Flores 2016

Results• Spinning necessary to transport fluid

to waste reservoir

• Goals: • Use calibration fluid • Reusable channels

© Israel Flores 2016

Future Plan• Disc design improvements

• Simplify microfabrication

• Easier to align sensing region

• 4 identical channels on each disk

• Functionalization of sensing region surface with bioreceptors

© Israel Flores 2016

Optical System

Tony Bujana

© Israel Flores 2016

Optical System

© Israel Flores 2016

Fabry-Perot Cavity

R: reflectance of partially reflecting mirrorL: distance between the mirrorsn: refractive index inside of the cavity

© Israel Flores 2016

Scaled Differential Calculation

ΔP1= -0.5262ΔP2= 0.39 Δη= 1.252

© Israel Flores 2016

ResultsCamera 1. 780nm Laser

Camera 2. 850nm Laser

© Israel Flores 2016

Future Plan (Bio-detection)

© Israel Flores 2016

Stopping Mechanism

Nathan Strachen

© Israel Flores 2016

Control System

• Precise positioning of disk• Closed loop control • User friendly

Rotate CD at high RPM for a specified

period of time Stop CD at a specific angle

© Israel Flores 2016

Our Approach

• Dual-Motor system - Stepper Motor - DC Motor - Pulley Connection

• Stepper motor allows for precise positioning • DC Motor enables rotation speeds up to

4600 RPM• IR sensor is used for reference point

© Israel Flores 2016

Implementation & Results

© Israel Flores 2016

Enclosure System

Stephen Sumrall

© Israel Flores 2016

Photonic Early Diagnoses

Optics

Sample

Detection

Processing

© Israel Flores 2016

Photonic Early Diagnoses

Combine different wavelength lasers and shooting them at the sample

The brains of the operation, interpret detection results from the process

Detecting changes in wavelength

Move sample through disc and position across laser beam

Optics

Sample

Detection

Processing

© Israel Flores 2016

Photonic Early Diagnoses Enclosure

Sample

Detection

Processing

Optics

© Israel Flores 2016

Sample

© Israel Flores 2016

Processing

© Israel Flores 2016

Photonic Early Diagnoses Enclosure

Optics

Sample

Detection

Processing

© Israel Flores 2016

Prototype I (2015) Prototype II (2016)

© Israel Flores 2016

System Integration

Israel Flores

© Israel Flores 2016

Up to Speed

© Israel Flores 2016

Progress Report• New Library

• Camera Interface• Successful Data

Frame Exchange

Team Friendly Software for rapid test script development

© Israel Flores 2016

Outlook

© Israel Flores 2016

Budget

© Israel Flores 2016

Gantt Chart Spring 2016

Enclosure System

Biodetection Fabrication technique

Microcomputer Stand alone system

Optics detection

Microfluidics Stage 1

Enclosure System Final Design

Biodetection

Microcomputer

Optics

Microfluidics

Final Device Complete5/19/1888 3/27/1924 2/3/1960 12/11/1995 10/18/2031

© Israel Flores 2016

…Thank You© Israel Flores 2016