Cellular Respiration and Fermentation...Anaerobic Respiration - Fermentation Occurs in the cytoplasm...

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Cellular Respiration and Fermentation

Cellular Respiration

● the process that releases energy from food in the presence of oxygen○ Oxygen must be available for use

Cellular Respiration Equation

6 O2 + C6H12O6 6 CO2 + 6 H2O + ATP

Remember!

We are not producing energy in cellular respiration

Instead, releasing it from organic molecules

Cellular Respiration Requirements

● Requires Oxygen and Glucose ○ Release CO2 and H2O

gradually ○ Must be gradual of energy

lost in form of light and heat■ There is a whole lot of

energy!!!

–http://www.youtube.com/watch?v=JOHdZsQXw7I

Stages of Cellular Respiration

● Glycolysis

● Krebs Cycle

● Electron Transport Chain

Step 1: Glycolysis

● Glucose is broken down in the cytoplasm

● Formed from each glucose○ 2 molecules ATP○ 2 molecules NADH ○ 2 pyruvate molecules

Step 2: Krebs Cycle (citric acid cycle)

● Pyruvate broken down into carbon dioxide during Krebs cycle

● Ultimate goal to make NADH and FADH2 for step 3 (electron transport chain)

Step 2: Krebs Cycle (citric acid cycle)

● Prior to the Krebs cycle pyruvate reacts with coenzyme A (CoA) to form Acetyl CoA○ Releases CO2 and NADH

● Acetyl CoA then moves to mitochondrial matrix

Step 2: Krebs Cycle (citric acid cycle)

● Krebs cycle begins with acetyl CoA combining with a 4-carbon compound to make 6-carbon citric acid

Step 2: Krebs Cycle (citric acid cycle)

● Citric acid is broken down ○ Releases 2 CO2○ Generates:

■ 1 ATP■ 3 NADH■ 1 FADH2

Step 2: Krebs Cycle (citric acid cycle)

● Remember: 2 molecules of pyruvate are formed ○ Krebs “turns” twice! ○

● For each glucose molecule the net yield is: ○ 6 CO2 ○ 2 ATP ○ 8 NADH ○ 2 FADH2

Needed for Next Step

Step 3: Electron Transport

● Step where most ATP is produced

● Electrons move along the mitochondrial membrane from one protein to another

Step 3: Electron Transport● As NADH and FADH2

release electrons, the H+ ions are released into mitochondrial matrix

● H+ ions are pumped across membrane

● Guess what it will be used for?

Step 3: Electron Transport

● ATP synthase is back again!

● Makes ATP when H+ diffuses down concentration gradient

Step 3: Electron Transport

● Electron transport chain produces: ○ 24 ATP ○ In eukaryotes one

molecules of glucose yields 36 ATP

Amoeba Sister’s Cellular Respiration

https://www.youtube.com/watch?v=4Eo7JtRA7lg

What if there isn’t enough oxygen!?

Anaerobic Respiration

● Occurs in low oxygen environments

● Some prokaryotes grow and reproduce without oxygen so they always do this!

Anaerobic Respiration - Fermentation

● Occurs in the cytoplasm and regenerates the cell’s supply of NAD+ while producing a small amount of ATP

● 2 types: ○ Lactic acid fermentation ○ Alcohol fermentation

Lactic Acid Fermentation

● Enzymes convert pyruvate from glycolysis to lactic acid

● Skeletal muscles do this during strenuous exercise when oxygen supply is low

Alcohol Fermentation

● Occurs in yeast and some bacteria

● Pyruvate converted to ethyl alcohol and CO2

Amoeba Sister’s Fermentation

https://www.youtube.com/watch?v=YbdkbCU20_M

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