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4.0 6.0
ABA1/2/3L/4
8.0 10.0 12.0 14.0 16.0
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ERG12 ERG10 Hygro ERG13tHMG1
ABA3L ABA1 KanMX ABA4ABA2
Empty vectors
ABA3L
Time (min)200 250 300 350 nm
0
1000
2000
3000
4000
232
278
Build FPP upregulating
construct
Reconstitute Botrytis cinerea
ABA pathway
Integrate constructs into
sYL12
Confirm ABA biosynthesis
(LC-MS)
Activate ABA sensor with
endogenous ABA
Deactivate ABA sensor with CYP707A1
L 1 2 3 4 5 6 7
2 3 4 5 6
L 2-3
3-4
4-5
5-6
L 3 4 L 5 6
L2+
33+
44+
55+
6
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