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Ms. Dawn J. Coleman Day Phone: 3609518520 Email: dawn.jovan.coleman@gmail.com Availability: Job Type: Permanent, Temporary, Internships Work Schedule: FullTime, PartTime
Desired locations: United States WA Olympia United States WA Tacoma
Work Experience: US Army Public Health CommandPacific JBLM MCHBRW Box 339500, MS 115 Joint Base LewisMcChord Tacoma, WA 98433 United States 10/2016 Present Hours per week: 25 American Red Cross Volunteer Duties, Accomplishments and Related Skills:
● Performs daytoday functions of temperatures and maintenance of biorepository machinery in order to meet strict accreditation criteria for the American Association for Laboratory Accreditation (A2LA) and accrediting bodies
● Assists with maintaining laboratory accreditation by adhering to quality control and quality assurance standards for laboratory functions and operations
● Assists and strictly adheres to laboratory safety and health requirements necessary for Biosafety Level2 laboratory procedures
● Performs molecularbased testing on environmental organisms in compliance with Standard Operating Procedures (SOPs) and Laboratory Information Database (LID) worksheets for technical and administrative processes IAW International Organization for Standardization (ISO)/International Electrotechnical Commission (IEC) 17025
● Performs and assists with maintaining a safe work environment through adherence to regulatory requirements in handling hazardous materials/waste Safety Data Sheets (SDS)
● Operates stateoftheart laboratory testing equipment to analyze vectorborne diseases ● Conducts molecularbased testing on environmental organisms such as mosquitoes, ticks, fleas,
and rodents using conventional and realtime polymerase chain reaction (PCR) and gel electrophoresis
● Performs data analysis on test results from various molecular instruments, reporting results to laboratory manager for review
● Maintains and organizes laboratory databases to support quality control record keeping and daily operations
● Assists with analysis of genetic material derived from disease agents found in zoonotic hosts and testing methods, ensuring that all testing complies with the requirements of the ISO/IEC 17025 and the A2LA requirements
Supervisor: Milagros Solá (2539663152) Okay to contact this Supervisor: Yes
Washington State Department of Ecology 300 Desmond Drive SE Lacey, WA 98503 United States 06/2015 09/2015 Hours per week: 25 Volunteer Duties, Accomplishments and Related Skills:
● Sorted microscopic biomasses from sediment samples that were collected from the Puget Sound waters
● Provided research on known biomasses and entered the information into an internal database Supervisor: Angela Eagleston, Marine Benthic Ecologist (3604076000) Okay to contact this Supervisor: Yes
Education: The Evergreen State College Olympia, WA United States Bachelor's Degree 06/2016 Credits Earned: 182 Quarter hours Major: Molecular Biology Minor: Biochemistry Relevant Coursework, Licenses and Certifications:
● Separation of complex biological specimens via handling and interpretation of gas chromatographymass spectrometry (GCMS), high pressure liquid chromatography, thin layer chromatography (TLC), fourier transform infrared spectroscopy (FTIR)
● Research in cell biology and protein biochemistry ● Microbe extraction and isolation, microbe streaking, Realtime PCR and RTPCR, protein
testing using fluorescent phenylboronate agarose gel electrophoresis ● Isolation of viruses and DNA from bacteria and vertebrates (procured from TESC biobank),
use of restriction enzymes, and hybridization techniques using PCR to identify alleles for genetic markers and repeat sequences
● Real time reverse transcriptase PCR
● Wet chemistry methods including titrations, pH, compound purification, analysis and characterization, distillation and reflux
● Quantitative methods and experimental design ● Bioassays, laboratory bench studies, scientific reports, maintaining research lab operations and
equipment ● Quantitative reasoning and scientific mathematics ● Descriptive and inferential statistics ● Introduction to forensic science with laboratory ● Microbial isolation of viruses from soil ● Conventional PCR and RTPCR ● Genetic sequencing, conventional PCR confirmation and organism speciation ● Extracted DNA from bovine heart and liver
SPECIAL PROJECTS SEAPHAGE program coordinated by the Howard Hughes Medical Institute and the use of bioinformatics tools on a bacteriophage genome project
● Collaborated with faculty collecting soil samples, using proper field methods in local area with interest in extracting a singular virus strain
● Over the course of 10 weeks, a soil sample was processed with an enrichment culture, filtered, diluted, plated, streaked, and purified
● Determined viral titers to successfully isolate a purified bacteriophage ● Work continued with the isolation and characterization of viral DNA by electrophoresis and
restriction enzyme analysis
South Puget Sound Community College Olympia, WA United States Some College Coursework Completed 06/2013 Credits Earned: 60 Quarter hours Relevant Coursework, Licenses and Certifications:
● American Sign Language I, II, III ● English Composition I, English Composition II, Basic Writing II ● Basic Math II, Elementary Algebra, Basic Algebra, Intermediate Algebra ● Analytical Reading
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