View
20
Download
0
Category
Preview:
Citation preview
1
2
3
EDTA
A2
PCV
4
CBC
EDTAEDTAmg
(19 – 23G)
cmcm
Safety box )(
A2
A2
A
pipette filler
C
5
Safety Box
g
6
A2
7
*
median cubital
*
*
*
8
9
EDTA
EDTA
(Ethylenediamin Tetera acetic Acid ) EDTA
EDTACBC
EDTA(EDTA –K2,2H2O EDTA
(EDTA –K3 EDTA (EDTA –NA2,2H2O)EDTA
EDTACBC
K3 EDTA
EDTA
, PCV , Hb , W.B.C , R.B.C MCV, Platelet
EDTA
EDTA-K3EDTA
PCV
MCHMCHC
EDTA
EDTA
EDTA
EDTA
10
EDTA
11
CBC
(duplicate
check test
Delta check
MCV,MCH,MCHC
12
13
Back ground
T-Brittin
Back ground
14
(Calibration Factor) =
CF= ×
SD SD SD
× 011
15
ــ - ــ
ــ
SD SD +_ SD
134
136
138
140
142
144
146
148
150
152
1 6 11 16
Day
g/l
Hemoglobin
(1) One control value outside the mean ± 2SD Warning
(2) One control value outside the mean ± 3SD Reject:SE or RE
(3) 2 consecutive controls exceed mean ±2SD Reject:SE
(4) 4 consecutive controls exceed mean +1SD or mean -1SD Reject:SE
(5)6 consecutive controls on one side of the mean Warning:SE
SE=Systematic error
RE=Random error
WBCRBC Hb HCT
T-
Brittin
+1s
+2s
+3s
-1s
-2s
-3s
16
tn = d √n sd
n
d
Sd
t
T-Brittin
مقدار هموگلوبين روز اول
مقدار هموگلوبين روز دوم
d d2
123 120 -3 9
135 139 4 16
176 181 5 25
155 150 -5 25
142 138 -4 16
d = -3 d) 2 = 9
d2 ) = 91
d- =d /5 = 3/5 = 0.6
n
-
Sd=
∑( d2) - (∑d)2
n-1
17
91 - 9/5
Sd= = 4.72 4 0.6
tn = 5 =0 .28 4.72
tt
(CV)
Replicate tests(CV)
CV
SD CV
SD (x-x- ) 2/ n-1
SD = 0.201 / 9 = 0.148
CV= SD / mean 100
CV=0.148/ 7.63100=1.9%
(Duplicate)
(Duplicate
±2SD
SD
(x-x- ) 2 x-x- WBC count
0.0009 -0.03 7.6
0.017 -0.13 7.5
0.029 0.17 7.8
0.0009 -0.03 7.6
0.017 -0.13 7.5
0.073 0.27 7.9
0.017 -0.13 7.5
0.0009 -0.03 7.6
0.017 -0.13 7.5
0.029 0.17 7.8
(x-x- ) 2 = 0.201x=76.3x- =7.63
18
SD= √∑d2
2n
Duplicate
2dd g/Lg/L
34.310
112
2
2
n
d SD=
2SD=6.7
2SD
(Check Test)(Duplicate)
SD
(MCHC,
MCH, MCV )
19
(Delta check)
Hb 2 g/dl
PCV 0.05 L/L
MCV >6 fL
MCH > 5 pg
WBC Normal to abnormal
Platelets Reduced or increased by more than 50%
Deviation Index (DI)
DI < 1
DI 1-2
DI 2-3
DI > 3
20
CBC
Phase Pre Analytical
CBC
Analytical Phase
Analytical PhasePost
CBC
K3EDTA
K3 EDTA
K3 EDTAK2 EDTA
MCVMCHC
/Lg/dl
/LSHb
21
A2
EDTA
HbA2
hourml
A2
A
HbA2
A2
A2
A2
A2
22
HbA2HbA2C
,E ,O arab(A'2) A2HbA2
A2C,E,OarabHbA2
E,C,G,D,S
23
A2
EDTA Heparin
gr/dl
)
A , F , S , C
F , A , S
C
24
PH
PH
Smear
(KCN)
Icebag
Hem) (
HbH
HbA
25
PCV
Packed cell volum (PCV)
PCV
PCV
PCV
Duplicate
K2EDTA
K3EDTAMCV
PCV±
26
(Plasma trapping)
EDTA
RCFgC
RCF
RPM
RCF= Relative Centrifugal Field
RPM = Revolution Per Minute
RCF=1.118 10-5 r RPM
2
27
Time PCV
Sample 1 Sample 2
2.0 0.40 0.59
2.5 0.39 0.58
3.0 0.38 0.57
3.5 0.38
(minimum packing time)
0.56
4.0 - 0.55
4.5 - 0.55
(minimum packing time)
28
(Class A)
NCCLS
KCN 0.05 g
K3Fe(CN)6 0.2g
KH2 PO4(Anhydrous) 0.140 g
Nonionic Detergent 05-1 ml
Clinical laboratory Reagent water
(type I) 1000 ml
EDTAmlmg
K3 EDTA
(Macro dilution)
29
g/L
30
Linearity
HiCN
(Stock)
g/l
2.09
Observed
OD
X
XExpected
Bias
Expected - observed
Bias = 100
Expected
31
(Na OH)
%Bias (OD expected)
(OD observed)
-0.91 2.075 2.094
-0.18 1.66 1.663
-1.12 1.245 1.259
1.97 1.037 1.017
0.48 0.83 0.826
- - 0.415
-2.1 0.207 0.212
32
(Drift)
(Stray light)
33
(Control button )
(Unwetted Tip)
34
mg/dl
mg/dl
mg
mg/dlmg/dl
stock
(CV%)
AA
1/101 11010
1/101 22020
1/101 2.5 2525
1/101 55050
1/101 10100100
1/112200200
1/11 5500500
1/11 1010001000
35
ExpectedBias
Bias % = ____________________________________________
(Tip-holder)
(O-ring)
36
HIV
37
Safety Box
Biohazard
( Sterilization)
Disinfection
(Waste Management)
.
Safety Box
Recommended