1

2

3

EDTA

A2

PCV

4

CBC

EDTAEDTAmg

(19 – 23G)

cmcm

Safety box )(

A2

A2

A

pipette filler

C

5

Safety Box

g

6

A2

7

*

median cubital

*

*

*

8

9

EDTA

EDTA

(Ethylenediamin Tetera acetic Acid ) EDTA

EDTACBC

EDTA(EDTA –K2,2H2O EDTA

(EDTA –K3 EDTA (EDTA –NA2,2H2O)EDTA

EDTACBC

K3 EDTA

EDTA

, PCV , Hb , W.B.C , R.B.C MCV, Platelet

EDTA

EDTA-K3EDTA

PCV

MCHMCHC

EDTA

EDTA

EDTA

EDTA

10

EDTA

11

CBC

(duplicate

check test

Delta check

MCV,MCH,MCHC

12

13

Back ground

T-Brittin

Back ground

14

(Calibration Factor) =

CF= ×

SD SD SD

× 011

15

ــ - ــ

ــ

SD SD +_ SD

134

136

138

140

142

144

146

148

150

152

1 6 11 16

Day

g/l

Hemoglobin

(1) One control value outside the mean ± 2SD Warning

(2) One control value outside the mean ± 3SD Reject:SE or RE

(3) 2 consecutive controls exceed mean ±2SD Reject:SE

(4) 4 consecutive controls exceed mean +1SD or mean -1SD Reject:SE

(5)6 consecutive controls on one side of the mean Warning:SE

SE=Systematic error

RE=Random error

WBCRBC Hb HCT

T-

Brittin

+1s

+2s

+3s

-1s

-2s

-3s

16

tn = d √n sd

n

d

Sd

t

T-Brittin

مقدار هموگلوبين روز اول

مقدار هموگلوبين روز دوم

d d2

123 120 -3 9

135 139 4 16

176 181 5 25

155 150 -5 25

142 138 -4 16

d = -3 d) 2 = 9

d2 ) = 91

d- =d /5 = 3/5 = 0.6

n

-

Sd=

∑( d2) - (∑d)2

n-1

17

91 - 9/5

Sd= = 4.72 4 0.6

tn = 5 =0 .28 4.72

tt

(CV)

Replicate tests(CV)

CV

SD CV

SD (x-x- ) 2/ n-1

SD = 0.201 / 9 = 0.148

CV= SD / mean 100

CV=0.148/ 7.63100=1.9%

(Duplicate)

(Duplicate

±2SD

SD

(x-x- ) 2 x-x- WBC count

0.0009 -0.03 7.6

0.017 -0.13 7.5

0.029 0.17 7.8

0.0009 -0.03 7.6

0.017 -0.13 7.5

0.073 0.27 7.9

0.017 -0.13 7.5

0.0009 -0.03 7.6

0.017 -0.13 7.5

0.029 0.17 7.8

(x-x- ) 2 = 0.201x=76.3x- =7.63

18

SD= √∑d2

2n

Duplicate

2dd g/Lg/L

34.310

112

2

2

n

d SD=

2SD=6.7

2SD

(Check Test)(Duplicate)

SD

(MCHC,

MCH, MCV )

19

(Delta check)

Hb 2 g/dl

PCV 0.05 L/L

MCV >6 fL

MCH > 5 pg

WBC Normal to abnormal

Platelets Reduced or increased by more than 50%

Deviation Index (DI)

DI < 1

DI 1-2

DI 2-3

DI > 3

20

CBC

Phase Pre Analytical

CBC

Analytical Phase

Analytical PhasePost

CBC

K3EDTA

K3 EDTA

K3 EDTAK2 EDTA

MCVMCHC

/Lg/dl

/LSHb

21

A2

EDTA

HbA2

hourml

A2

A

HbA2

A2

A2

A2

A2

22

HbA2HbA2C

,E ,O arab(A'2) A2HbA2

A2C,E,OarabHbA2

E,C,G,D,S

23

A2

EDTA Heparin

gr/dl

)

A , F , S , C

F , A , S

C

24

PH

PH

Smear

(KCN)

Icebag

Hem) (

HbH

HbA

25

PCV

Packed cell volum (PCV)

PCV

PCV

PCV

Duplicate

K2EDTA

K3EDTAMCV

PCV±

26

(Plasma trapping)

EDTA

RCFgC

RCF

RPM

RCF= Relative Centrifugal Field

RPM = Revolution Per Minute

RCF=1.118 10-5 r RPM

2

27

Time PCV

Sample 1 Sample 2

2.0 0.40 0.59

2.5 0.39 0.58

3.0 0.38 0.57

3.5 0.38

(minimum packing time)

0.56

4.0 - 0.55

4.5 - 0.55

(minimum packing time)

28

(Class A)

NCCLS

KCN 0.05 g

K3Fe(CN)6 0.2g

KH2 PO4(Anhydrous) 0.140 g

Nonionic Detergent 05-1 ml

Clinical laboratory Reagent water

(type I) 1000 ml

EDTAmlmg

K3 EDTA

(Macro dilution)

29

g/L

30

Linearity

HiCN

(Stock)

g/l

2.09

Observed

OD

X

XExpected

Bias

Expected - observed

Bias = 100

Expected

31

(Na OH)

%Bias (OD expected)

(OD observed)

-0.91 2.075 2.094

-0.18 1.66 1.663

-1.12 1.245 1.259

1.97 1.037 1.017

0.48 0.83 0.826

- - 0.415

-2.1 0.207 0.212

32

(Drift)

(Stray light)

33

(Control button )

(Unwetted Tip)

34

mg/dl

mg/dl

mg

mg/dlmg/dl

stock

(CV%)

AA

1/101 11010

1/101 22020

1/101 2.5 2525

1/101 55050

1/101 10100100

1/112200200

1/11 5500500

1/11 1010001000

35

ExpectedBias

Bias % = ____________________________________________

(Tip-holder)

(O-ring)

36

HIV

37

Safety Box

Biohazard

( Sterilization)

Disinfection

(Waste Management)

.

Safety Box