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248 Jean Avenue, Lyttelton
Centurion, South Africa
P.O. Box 14167, Lyttelton
Centurion, 0140, South Africa
Contact Person:
Company:
Disclaimer:
622196
VITALOXIDE
18/02/2020
15/01/2020
15/01/2020
KAMEELFONTEIN
PEBBLE ROCK GOLF ESTATE
Batch Number:
Active Substances:
Product Identification:
Product Concentrations:
EN 1276: BACTERICIDAL EFFICACY
Diluent Used in Test:
Expiry Date:
CHLORINE DIOXIDE
VITAL OXIDE
FINAL TEST REPORT
SANS 51276:2011 - Quantitative suspension test for the evaluation of bactericidal activity of chemical
disinfectants and antiseptics used in food, industrial, domestic and institutional areas.
200 MOONSTONE CRESCENT
TUMELO AFRICA (PTY) LTD
RENE STRYDOM Lab Number :
Analysis performed by: J. JACOBS
The results reported relate only to the samples tested and is expressed on an 'as received' basis unless
specified otherwise. The test report shall not be reproduced except in full, without written approval of the
Laboratory.
20g/L SODIUM THIOSULFATE + 5g/L LECITHIN
0.3g/L BOVINE SERUM ALBUMIN
Submit Date :
Sample Date :
Order reference :
Storage Conditions:
Recommended Diluent:
Appearance of Product:
HOMOGENOUS SUSPENSIONS
DILUTION NEUTRALISATION
AMBIENT TEMPERATURE OUT OF DIRECT LIGHT
2020/05/01
2017427437
NONE
SUITABLE FOR TESTING
STERILE DISTILLED WATER
NEAT
HOMOGENOUS SUSPENSIONS
Neutralizer / Rinsing Liquid:
Method:
Appearance during Test:
Interfering Substances:
Appearance of Dilutions:
Report Date :
0038
LAB Number: 622196 1 of 10 Vital Oxide
Conclusion:
Validations and Controls:
Johan Jacobs
Technical Advisor
All validations and controls were within specification during the test
Vital Oxide successfully achieved a 5-log reduction and achieved a >99.999% reduction in viable E.coli
ATCC10536, S.aureus ATCC6538, P.aeruginosa ATCC15442 and E.hirae ATCC10541 when applied at a
Ready-to-use concentration (80% of active substance in solution).
Tests were conducted under the obligatory conditions using 03g/L bovine serum albumin to simulate clean
conditions with a contact time of 5 minutes at a temperature of 20°C. All organisms were tested in duplicate
for verification.
LAB Number: 622196 2 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Test Temperature: 20°C
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Organism: Escherichia coli ATCC10536
Replicate: 1 of 2
Test Date: 28/01/2020
40 Vc2a 39Vc2b 26
Vc2a 23Vc2a 46 Vc2a
38 Vc1a 34Vc1b 24
Vc1a 26Vc1a 41 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 49.5 X̄ = 43.5 X̄ = 39 X̄ = 36.5
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
2.64E+08 log10N= 8.42
10-6258 266 N0 = N/10 log10N0= 7.42
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-726 31 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 3 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 2 of 2
Test Date: 28/01/2020
Test Temperature: 20°C
Organism: Escherichia coli ATCC10536
34 Vc2a 36Vc2b 26
Vc2a 23Vc2a 39 Vc2a
37 Vc1a 31Vc1b 24
Vc1a 26Vc1a 43 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 49.5 X̄ = 41 X̄ = 35.5 X̄ = 33.5
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
2.64E+08 log10N= 8.42
10-6258 266 N0 = N/10 log10N0= 7.42
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-726 31 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 4 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 1 of 2
Test Date: 28/01/2020
Test Temperature: 20°C
Organism: Staphylococcus aureus ATCC6538
90 Vc2a 78Vc2b 49
Vc2a 45Vc2a 99 Vc2a
84 Vc1a 73Vc1b 51
Vc1a 46Vc1a 93 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 95.5 X̄ = 96 X̄ = 87 X̄ = 75.5
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
4.05E+08 log10N= 8.61
10-6TNTC TNTC N0 = N/10 log10N0= 7.61
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-738 43 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 5 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 2 of 2
Test Date: 28/01/2020
Test Temperature: 20°C
Organism: Staphylococcus aureus ATCC6538
88 Vc2a 74Vc2b 49
Vc2a 45Vc2a 92 Vc2a
83 Vc1a 81Vc1b 51
Vc1a 46Vc1a 97 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 95.5 X̄ = 94.5 X̄ = 85.5 X̄ = 77.5
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
4.05E+08 log10N= 8.61
10-6TNTC TNTC N0 = N/10 log10N0= 7.61
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-738 43 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 6 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 1 of 2
Test Date: 05/02/2020
Test Temperature: 20°C
Organism: Pseudomonas aeruginosa ATCC15442
49 Vc2a 43Vc2b 28
Vc2a 35Vc2a 58 Vc2a
50 Vc1a 53Vc1b 29
Vc1a 31Vc1a 54 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 61.5 X̄ = 56 X̄ = 49.5 X̄ = 48
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
2.82E+08 log10N= 8.45
10-6288 281 N0 = N/10 log10N0= 7.45
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-727 24 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 7 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 2 of 2
Test Date: 05/02/2020
Test Temperature: 20°C
Organism: Pseudomonas aeruginosa ATCC15442
50 Vc2a 47Vc2b 28
Vc2a 35Vc2a 55 Vc2a
46 Vc1a 43Vc1b 29
Vc1a 31Vc1a 51 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 61.5 X̄ = 53 X̄ = 48 X̄ = 45
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
2.82E+08 log10N= 8.45
10-6288 281 N0 = N/10 log10N0= 7.45
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-727 24 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 8 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 1 of 2
Test Date: 05/08/2020
Test Temperature: 20°C
Organism: Enterococcus hirae ATCC10541
90 Vc2a 75Vc2b 47
Vc2a 56Vc2a 87 Vc2a
83 Vc1a 81Vc1b 51
Vc1a 49Vc1a 94 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 101.5 X̄ = 90.5 X̄ = 86.5 X̄ = 78
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
4.25E+08 log10N= 8.63
10-6TNTC TNTC N0 = N/10 log10N0= 7.63
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-746 39 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Lowest effective concentration NEAT
LAB Number: 622196 9 of 10 Vital Oxide
Validation and Controls:
Test Suspension and Test:
Summary:
Incubation Temperature: 37°C
Validation suspension Experimental condition Filtration control (B ) Method Validation (C )
(Nv0) control (A)
Replicate: 1 of 2
Test Date: 05/08/2020
Test Temperature: 20°C
Organism: Enterococcus hirae ATCC10541
84 Vc2a 71Vc2b 47
Vc2a 56Vc2a 93 Vc2a
86 Vc1a 76Vc1b 51
Vc1a 49Vc1a 97 Vc1a
X̄≥0.5XNv0 Spec X̄≥0.5XNv0
Complies Yes Complies Yes Complies Yes Complies Yes
Spec 30≤X̄≥160 Spec X̄≥0.5XNv0 Spec
X̄ = 101.5 X̄ = 95 X̄ = 85 X̄ = 73.5
4.25E+08 log10N= 8.63
10-6TNTC TNTC N0 = N/10 log10N0= 7.63
Test
Suspension
(N and N0):
N Vc1 Vc2 X̄ wm =
10-746 39 7.17 ≤ LgN0 ≤ 7.70?
>6.42 5 MINUTES
10% >300 >300 >3000 >3.18 <4.24 5 MINUTES
NEAT 0 0 <10 <1
Yes
ProductVc1 Vc2 Na = X̄ x 10 Log Na Log R Contact time
Conc.
Lowest effective concentration NEAT
LAB Number: 622196 10 of 10 Vital Oxide
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