HISTOLOGIA DA DENTINA

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DENTINADENTINA

DENTINADENTINA

COMPOSIÇÃOCOMPOSIÇÃO

COMPOSIÇÃOCOMPOSIÇÃO

Cristais de hidroxiapatita Carbonato, sulfatos Vestígios – F, Cu, Zn, Fe.

Cristais de hidroxiapatita Carbonato, sulfatos Vestígios – F, Cu, Zn, Fe.

COMPOSIÇÃOCOMPOSIÇÃO

PROPRIEDADES FÍSICAS PROPRIEDADES FÍSICAS

PROPRIEDADES FÍSICAS PROPRIEDADES FÍSICAS

PROPRIEDADES FÍSICAS PROPRIEDADES FÍSICAS

CARACTERÍSTICASCARACTERÍSTICAS

Avascular Prolongamentos dos odontoblastos Estrutura tubular

Avascular Prolongamentos dos odontoblastos Estrutura tubular

DENTINOGÊNESEDENTINOGÊNESE

DENTINOGÊNESEDENTINOGÊNESE

DENTINOGÊNESEDENTINOGÊNESE

Formação da matriz orgânica Formação da matriz orgânica

DENTINOGÊNESEDENTINOGÊNESE

Formação da dentina do manto Formação da dentina do manto

DENTINOGÊNESEDENTINOGÊNESE

Formação da dentina circumpulpar Formação da dentina circumpulpar

DENTINOGÊNESEDENTINOGÊNESE

Formação da dentina radicular

DENTINA PRIMÁRIADENTINA PRIMÁRIA

Dentina do manto Dentina do manto

DENTINA PRIMÁRIADENTINA PRIMÁRIA

Dentina circumpulpar Dentina circumpulpar

TÚBULOS DENTINÁRIOSTÚBULOS DENTINÁRIOS

Duplo “S” ou dupla curvatura Duplo “S” ou dupla curvatura

TÚBULOS DENTINÁRIOSTÚBULOS DENTINÁRIOS

TÚBULOS DENTINÁRIOSTÚBULOS DENTINÁRIOS

TÚBULOS DENTINÁRIOSTÚBULOS DENTINÁRIOS

TÚBULOS DENTINÁRIOSTÚBULOS DENTINÁRIOS

TÚBULOS DENTINÁRIOSTÚBULOS DENTINÁRIOS

ESPAÇO PERIODONTOBLÁSTICO

ESPAÇO PERIODONTOBLÁSTICO

DENTINA INTRATUBULARDENTINA INTRATUBULAR

Dentina intratubular

Dentina intratubular

DENTINA INTERTUBULARDENTINA INTERTUBULAR

Dentina intertubular

Dentina intertubular

DENTINA INTERGLOBULAR

DENTINA INTERGLOBULAR

DENTINA SECUNDÁRIADENTINA SECUNDÁRIA

DENTINA ESCLERÓTICADENTINA ESCLERÓTICA

Dentina intratubular

Túbulos ocluidos Túbulos parcialmente ocluidos

DENTINA REPARADORA ou

REACIONAL

DENTINA REPARADORA ou

REACIONAL

DENTINA REPARADORA ou

REACIONAL

DENTINA REPARADORA ou

REACIONAL

Secundária

- Fisiológica

- Altera o volume mas não altera a forma

Secundária

- Fisiológica

- Altera o volume mas não altera a forma

Reparadora ou reacional

- Patológica

- Altera o volume e a forma da polpa

Reparadora ou reacional

- Patológica

- Altera o volume e a forma da polpa

DENTINA REPARADORA ou

REACIONAL

DENTINA REPARADORA ou

REACIONAL

VERSUS

DENTINA TERCIÁRIADENTINA TERCIÁRIA

PRÉ-DENTINAPRÉ-DENTINA

LINHAS INCREMENTAISLINHAS INCREMENTAIS

LINHAS INCREMENTAISLINHAS INCREMENTAIS

LINHAS INCREMENTAISLINHAS INCREMENTAIS

von Ebner

CAMADA GRANULOSA DE TOMES

CAMADA GRANULOSA DE TOMES

JUNÇÃO AMELODENTINÁRIA

JUNÇÃO AMELODENTINÁRIA

JUNÇÃO CEMENTODENTINÁRIA

JUNÇÃO CEMENTODENTINÁRIA

SENSIBILIDADE DENTINÁRIA

SENSIBILIDADE DENTINÁRIA

Fim

F I M

The peripheral layer of dentin, which is the first layer of dentin deposited, is called mantle dentin. It is located adjacent to the enamel in the crowns of teeth and adjacent to the cementum in the roots. Notice the lines of Retzius in the enamel which are oriented at an angle to the dentinoenamel junction. Unstained, 100x  

                                                                                  

              

The dentinal tubules scribe an S-shape pattern. Primary dentin occupies the major area of the photomicrograph. A demarcation line (located toward the left border of the frame) clearly delineates the primary dentin from the more irregular secondary dentin. A small section of enamel is visible at the right of the field. Unstained, 40x  

                                                                                   

             

Higher power of the previous image. Unstained, 100x

                                                                                  

              

                                                                                   

             

                                                                                  

              

Higher power of image 17. Unstained, 100x

                                                                                  

              

Reparative dentin is produced in response to irritation. It is a variation of dentin in which the dentinal tubules are more irregular and fewer in number than in primary or secondary dentin. The pulp is located to the left in the field. Unstained, 40x

                                                                                  

              

Reparative dentin is produced in response to irritation. It is a variation of dentin in which the dentinal tubules are more irregular and fewer in number than in primary or secondary dentin. The pulp is located to the left in the field. (Higher magnification of image 20) Unstained, 100x

                                                                                  

              

                                                                                  

              

Another view of reparative (irregular) dentin. Unstained, 100x

                                                                                  

              

                                                                                  

              

                                                                                   

             

Another view of irregular dentin, this time at a cusp tip. Unstained, 40x

Another view of irregular dentin, this time at a cusp tip. (Higher magnification of image 23) Unstained, 100x

Dentinal tubules. Unstained, 100x

                                                                                  

              

Dentinal tubules. (Higher magnification of image 26) Unstained, 400x

                                                                                  

              

Dentin stained with a silver stain. Silver Stain, 40x

                                                                                  

              

Higher power of image 27. The dentinal tubules have lateral canaliculi which anastomose with those from adjacent tubules. The odontoblastic processes have lateral processes which extend into the canaliculi. Silver Stain, 200x

                                                                                  

              

                                                                                  

              

Higher power of image 28. The dentinal tubules have lateral canaliculi which anastomose with those from adjacent tubules. The odontoblastic processes have lateral processes which extend into the canaliculi. Silver Stain, 400x

                                                                                  

              

                                                                                  

              

The terminal ends of the dentinal tubules branch at the dentinocementum junction. Silver Stain, 400x

                                                                                  

              

                                                                                  

              

                                                                                  

              

Identify dentin, predentin, odontoblasts and odontoblastic processes (Tomes' dentinal fibrillae). Silver Stain, 400x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

Cross section of dentinal tubules. The cut ends of the odontoblastic processes are visible. Silver Stain, 400x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

Cross section of dentinal tubules with odontoblastic processes. H&E, 400x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

Notice the contour lines of Owen in dentin and the lines of Retzius in enamel. The two tissues are artifactitiously separated at the dentinoenamel junction. Unstained, 40x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

The lines of von Ebner are oriented in the horizontal axis at approximately right angles to the dentinal tubules which run in the vertical axis. Unstained, 40x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

The lines of von Ebner are oriented in the horizontal axis at approximately right angles to the dentinal tubules which run in the vertical axis. (Higher magnification of image 36) Unstained, 100x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                        

Another view of the incremental lines of von Ebner. Unstained, 100x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                 

Another view of the incremental lines of von Ebner. (Higher magnification of image 38) Unstained, 200x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

The photomicrographs depict an occlusal area of a tooth with a brown central pit visible in frame 40. The regions of interglobular dentin appear as black spaces which are located in the crown of the tooth near the dentinoenamel junction. Notice that the dentinal tubules traverse the interglobular dentin. How is interglobular dentin formed? Unstained, 40x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

The regions of interglobular dentin appear as black spaces which are located in the crown of the tooth near the dentinoenamel junction. Notice that the dentinal tubules traverse the interglobular dentin. How is interglobular dentin formed? (Higher magnification of image 40) Unstained, 200x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

Tomes' granular layer appears as a black layer of granular structures which are located in the peripheral zone of root dentin adjacent to the cementum. Unstained, 100x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

                                                                                  

              

Higher power of image 42. Unstained, 200x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

                                                                                  

              

                                                                                  

              

Groups of dentinal tubules which are filled with air appear black in ground sections of dentin when viewed with transmitted light. How are dead tracts formed? Unstained, 40x

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

                                                                                  

              

                                                                                  

              

                                                                                  

              

During caries removal, carbide burs remove healthy dentin, exposing many dentinal tubules.

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

                                                                                  

              

                                                                                  

              

                                                                                  

              

During caries removal, SS WHITE SMARTBURSTM instruments leave healthy dentin intact, exposing fewer dentinal tubules.

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

                                                                                  

              

                                                                                  

              

                                                                                  

              

                             

             

                       

            

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                  

              

                                                                                                                                                                                                                          

                                                                                  

              

                                                                                   

             

                                                                                  

              

                             

             

                       

            

COLORAÇÃO POR TETRACICLINA

A. Junctional epithelium B. Contour lines of Owen C. [Dentogingival fibers of gingival lamina propria] D. [Dentoperiosteal fibers of gingival lamina propria] E. [Circular fibers of gingival lamina propria] F. [Alveolar crest fibers of periodontal ligament]