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LUCIFERASE
A. Cengiz Öztireli
BIOLUMINESCENCE
BIOLUMINESCENCE (LIVING LIGHT)Milky Sea
BIOLUMINESCENCE
Applications
BIOLUMINESCENCE
Sea AnemonesBacteria Dinoflagellates
BIOLUMINESCENCE
FIREFLYES (PHOTINUS)Attracting matesAttracting prey
LUCIFERASE
FIREFLY BACTERIA
BIOLUMINESCENCE
8 ATP → 1 Green Photon
Reaction:
LuciferinLuciferase + O2 Inter. P* P + hv
ATP
BIOLUMINESCENCE
Color P*
P* + F → F* + P Proteins around Structure
PHOTINUS PYRALIS (FIREFLY)
PHOTINUS PYRALIS LUCIFERASE
Luciferase + Luc-COOH
ATP
Luciferase: Luc-CO-AMP
O2
Luciferase: Luc=O*
PHOTINUS PYRALIS LUCIFERASE
N-Terminal
C-Terminal
Cleft
Structure and Functioning
CONCLUSIONS
THANK YOU
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