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Materials and MethodsPhase 1
• Evaluated acute effects on fasting blood glucose and on post-oral glucose
• Groups 1 and 3 received distilled water• Groups 2 and 4 received cinnamon extracts• All groups fasted for 12 hours overnight and
blood glucose levels were measured fasting and post-oral glucose load– This cycle was repeated 3 times to obtain mean
blood glucose values
Cinnamon Extract Preparation
• Dosage for rats calculated according to body surface area
• Daily cinnamon dose for a 200g rat was 120mg in 1 ml of distilled water via a metal oro-gastric tube
• Dose(mg/kg)= Human dose (mg/kg) x [human factor/Rat factor]
Results
• No statistically significant difference in mean body weight and mean blood glucose levels at each hour of groups 1 and 2
• Only the cinnamon administered group demonstrated a greater mean percentage difference at 0.5, 1 and 2 hour, but was still not statistically significant.
• Comparing groups 3 and 4 at 0.5 hour, group 4 demonstrated a faster decline in blood glucose.
Results Continued
• The mean percentage reduction in blood glucose from 0.5 to 1 hour in groups 3 and 4 were 7.7% and 27.7% respectively.
• The cinnamon group also demonstrated a lower blood glucose value at 8 and 12 hours post oral glucose load
Fasting and Post-oral glucose load levels
.5, 8, and 12 hours were all statistically significant
Groups 1 and 2 Results
• There was no statistical difference between Groups 1 and 2 on fasting blood glucose
Groups 3 and 4 Results
• Group 3 (distilled water) declined slower than Group 4 (cinnamon extract)
Materials and MethodsPhase II
• Evaluated the effects on food consumption, body weight, blood glucose, and lipids over 1 month
• 4 different groups: – Healthy rats
• Group A: 8 subjects, distilled water• Group B: 8 subjects, cinnamon extracts
– Diabetes induced rats• Group C: 5 subjects, distilled water• Group D: 5 subjects, cinnamon extracts
Diabetes Induction
• Groups C and D were induced with diabetes by a single intravenous injection of streptozotocin to the tail vein at a dose of 75 mg/kg body weight
• Tests were performed 14 days after injection to confirm and rats with a fasting blood glucose of >200mg/dl were allocated to Groups C and D
Results: Body weight and Food Consumption (Groups A & B)
• The mean weight of the control group increased significantly (Group A) in comparison with Group B.
• Mean food consumption of both Groups A and B had no significant difference between day 0 and day 30
Results: Body Weight and Food Consumption (Groups C & D)
• The weight reduction in both Groups C & D were not statistically significant
• Mean food consumption in Group D (cinnamon group) significantly reduced from day 0 to 30
• Mean food consumption in Group C (distilled water) did not significantly reduce
Results: Mean Food Consumption
• Mean food consumption during study period (A) in healthy animals and (B) in diabetes induced
Results: Blood Glucose
• No statistical difference in healthy rats on fasting blood glucose levels and the 2 hour post-prandial blood glucose.
• In the diabetes induced animals, the fasting blood glucose was significantly lower in the cinnamon group on day 20 and 30.
• Also in the diabetes induced animals the 2 hours post-prandial blood glucose was significantly lower on day 30 in the cinnamon group
Results: Lipids
• In both healthy and diabetes induced animals, the cinnamon group showed significantly lower total in LDL cholesterol levels on day 30 compared to day 0.
• This difference was not observed in the control groups (Groups 1 & 3)
• HDL cholesterol and triglycerides remained unchanged in all 4 groups
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