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Role of cytokines and lymphocytes on the efficacy of
Posaconazole and Benznidazole in treatment of
experimental acute Trypanosoma cruzi infection
Alvaro José Romanha
Centro de Pesquisa René Rachou – FIOCRUZ - MG
Rio de Janeiro, July 2009.
WT
IFN- KO
IL-12 KO
WT
IFN- KO
IL-12 KO
Mortality, survival and cure of WT, IFN-γ-KO and IL-12-KO mice infected with T.cruzi Y strain and treated with posaconazole and benznidazole
Treatment
Mortality, survival and cure of WT, IFN-γ-KO and IL-12-KO mice infected with T.cruzi Y strain and treated with posaconazole and benznidazole
Mortality, survival and cure of WT, IFN-γ-KO and IL-12-KO mice infected with T.cruzi Y strain and treated with posaconazole and benznidazole
Conclusion:
Our results demonstrate that either IFN-γ or IL-12 deficiency reduces
the efficacy of POS or BZ in the acute murine infection with T.cruzi but
also indicate that the anti-T.cruzi activity of POS is much less
dependent on the activity of IFN-γ than that of BZ.
WT
LTCD4+ KO
LTCD8+ KO
LB KO
WT
LTCD4+ KO
LTCD8+ KO
LB KO
Survival and cure of WT and CD4+ T-lymphocyte, CD8+ T-lymphocyte, and B-lymphocyte-KO mice infected with the Y strain of T.cruzi and treated with POS or BZ
Conclusion:
The absence of different lymphocytes’ activities has distinct effects
on the efficacy of POS and BZ in the acute murine infection with
T.cruzi, probably reflecting different parasite stages preferentially
targeted by the two drugs and distinct cooperation patterns with the
host immune system.
Collaborators:
Marcela L. Ferraz e Rosana O. AlvesLaboratório de Parasitologia Celular e Molecular, Centro de Pesquisa René Rachou,
FIOCRUZ-MG;
Ricardo T. GazzinelliLaboratório de Imunopatologia, Centro de Pesquisa René Rachou, FIOCRUZ-MG e
Departamento de Bioquímica e Imunologia, ICB-UFMG;
Julio A. UrbinaLaboratório de Química Biológica, Centro de Biofísica y Bioquímica, Instituto Venezolano de
Investigaciones Científicas, Venezuela
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