Salk Institute Mobile Lab Gel Electrophoresis Student Instructions Edvotek set up Salk Institute...

Salk Institute Mobile Lab

Gel Electrophoresis

Student Instructions

Edvotek set up

Gel Electrophoresis Student Station Set-up

tip container w/tips

waste container

5µl fixed pipettor

practice sheet in protector

foam epi rack w/ 1 food color tube each: orange, green, purple

How to Use a 5µl fixed pipettor

• Make sure a yellow tip is firmly twisted onto the end of the red pipettor

• Carefully open one of the food color tubes – Don’t let the lid snap back

and splash you!

• Hold down the button on the top of the pipettor

• While holding the button down, insert the tip into the food color tube

• Release the button and remove from the food color tube

• The tip now contains just 5 microliters of solution!

• To dispense the solution push down on the button again

• Using your other hand to guide the end of the tip, practice dispensing the 5µl drops – Try to not push down on

the plastic sheet - see if you can hold the pipettor tip just above the paper

• Twist off the dirty tip into the waste container and replace with a clean tip

• Always use a clean tip each time!!

• Ready to load a real gel?

• Load the food color solutions into the wells on your group’s gel

• Be careful not to poke through the bottom of the wells!

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