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(A) 50. (T) 50. (C) 50. (G) 50. A. Oligo 3. B. Oligo 1. Oligo 2. 3.1. 3.1. 3.1. 3.1. 9.4. 9.4. 9.4. 9.4. 1.3. 1.3. 1.3. 1.3. 0. 0. 0. 0. 9.4. 9.4. 9.4. 3.1. 3.1. 3.1. 1.3. 1.3. 1.3. SSB ( µ M):. SSB (µ M):. 0. 0. 0. SSB- DNA. SSB-ssDNA. Free DNA. ssDNA. - PowerPoint PPT Presentation
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SSB -oligo
20-ntoligo
50-ntoligo
21 3 4 5 6 7 8 109 11 12 13
Excess cold 50-nt oligo
5 10 20
50
No
SS
B0 10
0
Excess cold 20-nt oligo
10 20 50
No
SS
B0 10
0
SSB-DNA
Free DNA
SSB (µM): 0 1.3 3.1
9.4
15 nt 20 nt 30 nt 40 nt 50 nt
0 1.3 3.1
9.4
0 1.3 3.1
9.4
0 1.3 3.1
9.4
0 1.3 3.1
9.4
1 2 3 74 5 6 8 9 10 11 21 31 41 51 61 8171 0291
SSB-DNA
Free DNA
SSB (µM):
1 2 3 74 5 6 8 9
(A)50
0 1.3 3.1
9.4
(T)50 (C)50 (G)50
0 1.3 3.1
9.4
0 1.3 3.1
9.4
0 1.3 3.1
9.4
6101 11 21 31 41 51
SSB (µM):
Oligo 1
SSB-ssDNA
ssDNA
0 1.3 3.1 9.4
Oligo 2 Oligo 3
1 2 3 74 5 6 8 9 10 11 12 13
0 1.3 3.1 9.4
0 1.3 3.1 9.4
A B
C D
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