Supercritical fluid methods Jiří Konečný 2005. Sample is solid phase SPME (Solid phase micro...

Supercritical fluid methods

Jiří Konečný

2005

Sample is solid phase

SPME (Solid phase micro extraction)Sorbent is trapping contaminans from gas.

Biologic materials

Earth

CFE - Supercritical fluid extraction

Oven

Sample

Restrictor

CO2

Ectract + CH3OH

Extractioncell

Supercriticalfluid

cilinder

Pump

CO2

CH3OH

SFC - Supercritical fluid chromatography

EctractInjector

Pump

CO2

Supercriticalfluid

cilinder

CO2

Waste

Chromatogram

Oven Detector

Collum

InterfaceRestrictor

Why use supercricitcal methods?

density

g/ml

Difuzion coeficient

dinamic viscosity

coeficient

presure

GC 0,2 10-5 10-1 0,5-2 MPa

CFC 600 10-8 10-4 to 65 MPa

LC 800 10-9 10-3 10-40 MPa

Propertis of medium are difrent.Sample is better elution.

Using

Analytical chemistry – search for new substance and analyzes concaminans in eviroments

Industry – izolation of parfume and special substaces in famakology

Information sources

• Lecture analytical chemistry by Prof. RNDr. Sommer, DrSc

• Prof. RNDr. Sommer, DrSc : Základy analytické chemie• www.ashlandanalytical.com/ tour-sea.htm

• http://www.pioneerherbs.com/super_critical.htm