The Nucleus Text and image sources are included using the notes function of the.ppt file

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The Nucleus

Text and image sources are included using the notes function of the .ppt file

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Interphase Nuclear Dynamics

DIC Brightfield Euchromatin

Time Lapse Video

EM thin

section view

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Chromatin organization

early replication -> late replication chromosomal organization one cell cycle later

lamin B and late replicated DNA

lamin B and early replicated DNA

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Packing it

all in

146 bp; 6x condensation

40x condensation

The HistoneCode

Anchoring the genome

LAPs (Lamin associated proteins) anchor the lamina to the inner envelope

Freeze etch view of the lamina

The nuclear matrix

Diversity of nuclear shapes

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Eosinophil nuclei are constricted

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Chromosomal domains

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Nuclear compartments

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Promyelocytic leukemia (PML) nuclear bodies

Video

Total RNA production (fluorouridine pulse)

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Nucleolar Substructure

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Mammalian cell

Yeast cell

Polymerase I transcribes

rDNA in nucleolar fibrillar centers

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500 nm

Gene Expression Factories

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Animated reconstru

ction

Euchromatin (acH3), DNA (DAPI), Spliceosomal protein SC-35

Spliceosomes

contain poly-A RNA

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Nuclear speckle dynamics

Time lapse sequences show SC-35 protein over 30 min periods

3 Video Sequences

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NuclearmRNA

localizations

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Collagen A1 mRNA

Epstein-Barr Viral mRNAs

mRNA (red)Gene (green)

Nuclear traffic

Visualizing

Nuclear Pores

Negative Stain

Thin Section

Freeze Fracture

CryoEM Tomographic Reconstruction

Annulate lamellae store pores

400 nm

Nuclear pores

In vitro regulation by Ca++

Transport of nucleoplasmin-coated gold particles

It takes two (or three) to tango

Digitonin-permeabilized cells incubated with NLS*BSA*TRITC

-cytosol +cytosol +cytosol, GTP

NLS*BSA + cytosolic

factorsidentifies

nucleoporins on a

Western-style blot

Nup358Nup214Nup153

Nup 98

Nup 62

Nuclear Transport

Ran*GDP

Ran*GTP

Ran*GDP

Ran*GTP

Cytoplasmic compartment Nuclear compartment

Ran-GEFRan-GAP

Exportin

NESExportin

NES

NLS ImportinNLS Importin

FG affinity transport

Follow the FG-repeatsFollow the FG-repeatsFollow, follow, follow, follow

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Animation

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The nucleus - not just a place to find DNA

anymore