Welcome to Biology 523: Histology Please take and fill out an index card: Side 1: large print-->...

Welcome to Biology 523: Histology

Please take and fill out an index card:

Side 1: large print--> first name & last initial

Side 2: name:major:

career goal: your hopes for the course: information about you that I should know:

Biology 523: Histology, 4 credits

Instructor: Dr. Elizabeth Connor 353 Morrill IV South telephone: 545-4855 email: econnor@bio.umass.edu office hours: by appointment

ANNOUNCEMENTS

Flu can include: fever, cough, sore throat, runny or stuffy nose, body aches, headache, chills, and fatigue

Preventing flu

• Get vaccinated.

• Wash your hands often.

• Cough or sneeze into a tissue or your elbow instead of into your hands.

• Avoid touching your eyes, nose or mouth, to reduce the spread of germs.

• Don't share food, drinks, utensils and other similar items.

• If you’re sick, stay home!!! Email me about your situation and we will work it out.

OBJECTIVES

#1- ANATOMY: To understand the structural basis for the functioning of a living organism

Close relationship between structure and function

ANATOMY

GROSS ANATOMY (macroscopic)

HISTOLOGY (microscopic)

OBJECTIVES

#2- TECHNICAL ADVANCES: To appreciate that our ability to answer questions is driven by the development of new technology

Why study histology?

What are the uses of histological techniques and microscopic tissue identification?

Tools of the Histologist

Requirements:

Tissue PreparationStart with an organism:

Organ->Tissues-> Cells-> Molecules

fixation/sectioning/ staining

Imaging (Microscopy)

Interpretation - cell biology - physiology - structure of tissues - organ structure/function

Visual Integration**A challenging skill**

Teaching Assistants:

Bryan Olson bolson@nsm.umass.edu

Undergraduate Lab Assistants

Required Textbook: Histology: A Text and Atlas, Ross, Kaye, and Pawlina 6th Edition, Lippincott Williams & WilkinsISBN 0-683-30242-6

*** BRING TEXTBOOK TO LAB ***

Jejenum

Many free online histology materials are available!

Links on Course Webpage

Recommend Histology WorldRecommend MedPics UCSD

Grading: 300 total possible points

Two 1 hour in class exams (55 points each) 110

Final Exam 60

Homework Problems 10

Laboratory Attendance 15

Three Laboratory Practical Exams 55 ( 15, 15, 25 )

Contributions to Images2012 Gallery 20

Group Oral Project Presentation 10

Individual Written Project Report (2 parts) 20

Laboratory Experience (120 Points)

Exercises using Prepared SlidesAssessment: Three Practical Exams (15, 15, 25

points)

Group Lab Project #1

As a class, we will assemble Images2012, an on-line image gallery of tissues, cells and organs examined during the course. (20 points)

>2500 images from previous class efforts also available on our course website

Assessment: Evaluation of postings to Images2012 (20 pts)

Accuracy, labeling, quality of image

Group Lab Project #1

Images2012 Gallery: Your work is protected by-

“Please Note: The imagery created by students in this course is licensed under the Creative Commons Attribution- NonCommercial - ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ or send a letter to Creative Commons, 444 Castro Street, Suite 900, Mountain View,

California, 94041, USA.”

Laboratory Experience (120 Points)

Group Lab Project #2

Sectioning and staining of frozen and paraffin-embedded tissue of a rat organ. Techniques: microtome sectioning, frozen sectioning with cryostat, staining, immunohistochemistry, fluorescence microscopy, bright field microscopy, imaging, image processing.

Assessment: Group Oral Presentation: In class (10 pts) Individual Written Project Report: (20 pts)

First lab:Tuesday, January 31st or

Wednesday, February 1st

To prepare for lab-

Read Lab 1: Microscopy and Imaging Think about project partners (3/group)

Secrets of Success

-Attend every class and every lab.

-Come to class and lab prepared.

-Ask questions.

-Complete all assignments.

-Review & study material after each lecture.

-Answer review questions.

-Attend help and review sessions.

-Identify study partners.

Course Website!

TIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETIMETI

ACCESS TO ISB 264

Exceptions: it will be in use by other courses on:

Monday and Thursday 1:25pm-4:25pmFriday 3:35pm-4:35pm

……….And some other times to come

Challenge:

Identify tissues. (Epithelial, Nervous, Muscle, Connective Tissue)

Identify composite of tissues as an organ.

Use appropriate descriptive language and terms.

Understand the appropriate use of techniques to study material at the histological level

Orientation and Plane of section

Orientation and Plane of section

CROSS SECTION

LONGITUDINAL SECTION

Challenges of Interpreting Plane of Section

= Organ = Surrounding Tissues

Challenges of Interpreting Plane of Section

= Organ = Surrounding Tissues

Staining techniques

Magnification, Details of structure