Bioo Scientific - Increase Your Multiplexing Capacity for SeqCap Target Capture

Increase Your Multiplexing Capacity for SeqCap

Target Capture

Roche NimbleGen’s SeqCap EZ Library target enrichment system is a...

Solution-based capture method & enables enrichment of the whole exome

or custom regions of interest

One of the biggest drawbacks of the SeqCap EZ Library target enrichment

system is that there are...

Only 24 barcodes and blockers available for multiplexing

And they are supplied lyophilized which need to be resuspended

Bioo Scientific has increased the ease and multiplexing capability of the

SeqCap target capture baits with the...

NEXTflex™ Pre-Capture Combo Kit(NimbleGen SeqCap Compatible)

The NEXTflex™ Pre-Capture Combo Kit offers up to 96, ready-to-use

barcodes and blockers for multiplexing

Increasing the number of samples you can multiplex from 24 to 96

Reducing the cost of targeted sequencing dramatically

The NEXTflex™ Pre-Capture Combo Kit is a complete Illumina-compatible

library prep solution

Which integrates with the SeqCap Target Capture System

Workflow Stage Reagents Needed Bioo Supplied NimbleGen Supplied

1. Library Preparation

NEXTflex DNA-Seq Library Reagents ✓

NEXTflex DNA Barcodes ✓

2. Hybridize Libraries to Probes

NimbleGen SeqCap Target Capture Baits ✓

NEXTflex DNA Barcode Blockers ✓

NimbleGen SeqCap Hybridization & Wash Kit ✓

NimbleGen SeqCap Pure Capture Bead Kit ✓

3. Amplify Captured Libraries NEXTflex Post-capture Amplification Reagents ✓

The kit has also been validated by both Bioo Scientific and Roche for use with

SeqCap Target Capture

Mapping & Coverage Statistics of the NEXTflex™ Pre-Capture Combo Kit

TargetedBases

63,564,965

Mapped Readson Target

75.6%

Raw ReadsMapped

80.0%

AverageCoverage

43.2x

RawReads

76,154,408

Sensitivity DetectingKnown SNPs

96.8%

Unique ReadsMapped

75.5%

Specificity DetectingKnown SNPs

99.2%

Four NEXTflex libraries were prepared from 1 µg of NA19238, NA19239, NA19240, or NA12762genomic DNA with NEXTflex index adapters using standard NEXTflex protocols. EZ Exome v3.0

targets were enriched from these multiplexed libraries.

To learn more about the validation view the whitepaper: An Integrated Solution to

Simplify Library Preparation & Multiplexing for NimbleGen Sequence Capture

DOWNLOAD WHITEPAPER

If you have any questions email us atnextgen@biooscientific.com

or visit our website atBiooScientific.com/SeqCap