Saci Short Description And Applications

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Surface Activated Chemical Surface Activated Chemical Ionization – applications to low Ionization – applications to low molecular weight drugs molecular weight drugs analysisanalysis

Simone CristoniSimone Cristoni

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Surface Activated Chemical Ionization

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Surface Activated Chemical Ionization

Controlled, low

potential

Metal surface

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SACI

High sensitivity

High Versatility (different eluent flow rates, compounds and matrix)

Cost effective (avoid pre purification step working by sample dilution)

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Analysis of addictive drugs

Urine

Hair

Saliva

Sample dilution 1:10

Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow

gradient chromatography

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Analysis of addictive drugs

Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) Acquisition mode: tandem mass spectrometry (MS/MS) Chromatography column: C18 50x4.1 1.8 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA Eluent flow: 500 µL/min Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes,

from 40 to 5 % in 2 minutes. Sample volume injected: 20 µL

Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow

gradient chromatography

Experimental conditions

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Analysis of addictive drugsUrine 2 ng/mL

APCI

SACI

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Analysis of addictive drugsSaliva

4 Retention time (min)

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a) SACI

Sig

nal

in

ten

sity HCT

0

5.3E+5 12.40

LTQ

4 Retention time (min)

14

a) SACI

Sig

nal

in

ten

sity HCT

0

4.1E+4

LTQ

40

Sig

nal

in

ten

sity

3.2E+2

Retention time (min)

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b) APCI

HCT

LTQ

Sig

nal

in

ten

sity

0

1.89E+2

Retention time (min)

14

c) ESI

4

HCT

LTQ

0

Sig

nal

in

ten

sity

5.6E+2

Retention time (min)

14

b) APCI

4

HCT LTQ

Sig

nal

in

ten

sity

4.2E+3

Retention time (min)

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c) ESI

04

LTQHCT 12.20

12.30

10 ng/mL of 6-MAM 1 ng/mL of 6-MAM

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Analysis of pesticides

Water Spinach Sample dilution 1:10

Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow

gradient chromatography

Different matrix

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Analysis of pesticides

Mass spectrometer: ESI-TOF (Bruker Daltonics) Acquisition mode:High mass accuracy full scan MS Chromatography column: C18 50x4.1 3,5 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3OH + 0.1% FA Eluent flow: 500 µL/min Gradient: %B: 5 for 5 minutes, from 5 to 40 % in 10 minutes,

from 40 to 5 % in 2 minutes. Sample volume injected: 20 µL

Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow

gradient chromatography

Experimental conditions

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Analysis of pesticideswater

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Analysis of pesticidesspinach

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Analysis of toxin in food

Wine Grape Sample dilution 1:10

Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow

gradient chromatography

Different matrix

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Analysis of pesticides

Mass spectrometer: HCT Ultra Ion Trap (Bruker Daltonics) Acquisition mode: tandem mass spectrometry (MS3) Chromatography column: C18 250x4 5 µm i.d. Eluents: A:H2O + 0.1% FA %B :CH3CN + 0.1% FA Eluent flow: 1mL/min. Gradient: %B: 5 for 5 minutes, from 5 to 70 % in 10 minutes,

from 70 to 5 % in 2 minutes. Sample volume injected: 20 µL

Avoiding pre-concentration step and matrix effect thanks to both high SACI sensitivity and medium High Flow

gradient chromatography

Experimental conditions

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Analysis of pesticidesspinach

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Analysis of OTAWine – 10 ng/mL

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Conclusions

High sensitivity (LOD, LOQ, linerity ranges) Cost effecting avoiding matrix effect and pre-concentration step

(e.g. Solid Phase Extraction) Possible to use it in a wide range of chromatographic conditions. High throughput screening of complex mixture if coupled with

high mass accuracy analyzers (e.g TOF or FTICT).

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Future developments

Ion exchange chromatography (low operative voltages employed in SACI makes possible to operate in these chromatographic conditions).

High sensitive quantitation analysis of peptide and protein drugs (ioni monocarica; TOF).

Pesticides (developing high sensitive methods for screening of selected groups of pesticides of interest)

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SACI demo site

Web site: http://www.isbiotechnology.com

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acknowledgment

Dr.ssa Sara Crotti – ISB Milan Dr. Lorenzo Zingaro – ISB Milan Dr.ssa Cristina Canton – ISB Milan Dr.ssa Laura Molin – ISB Milan Dr. Pietro Traldi – CNR Padova Prof. Luigi Rossi Bernardi – University of Milan Dr. Simone Rubini – Bruker Daltonics Dr. Elisa Basso – Bruker Daltonics Dr. Leopoldo Dimiziano – Bruker Daltonics

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www.bdal.com