CDC20064

NTP

2000 18.6%

46.5%

27.8%

20007.6%

17.1%

10.7%

617.6% 17.3% 16.6% 2.8% 1.5% 1.3%)

8

% 1996 35.0 66.0 40.52001 29.8 60.8 35.5 35.7 65.3 44.8 42.1 55.8 43.3 17.6 50.0 24.5 17.5 44.5 23.3 14.8 59.3 21.9 13.0 38.1 15.5

8

8

% 1996 14.6 43.5 19.72001 9.6 42.6 15.3 9.8 51.0 21.2 11.4 29.1 13.1 3.8 23.2 7.9 3.8 26.9 8.8 6.5 45.0 12.6 3.5 22.2 5.3

(Mycobaterium)1007(M.tuberculosis).7

Nontuberculosis Mycobacteria,NTM

Rubyun

-

37

37PH6.8~7.210~1810~30

(1)

(2) (3).

()1~24%NaOH)1min15~20min

0.5~1ml3000r/min30min[2~42%NaOH)15min] 4~5h,10ml,3000r/min30 min,[4%H2SO4,15min].

()

() 4%H2SO41325min)0.1mlL-J225min, .

()

,24h,L-J; 3000r/min30 min,L-J.,[4%NaOH),10~15min]L-J

,;,3000r/min10 min;[2~44%H2SO4],15~20 min)L-J. ,, 4%NaOH),15 min.

1~24%NaOH1~2min15min0.1ml237

3,7,

,8

xg++/12601.87.10.2530004.511.20.4038009.611.60.83

1.37118

2.2%~5%

(1):,,.(2)1/4(3)21/2(4)33/4(5)4(6):1/4

20.

10~100/

2~87~8

,

Bectec -TB64014C14CCO2CO24~25

Bectec-tb9607H9O7~4260

Bect/alert 3DCO2 CO2 CO210

3~6

G. Meissner () 1964,30 G. Canetti J.Grossete1963 ,WHO .D.A.Mitchison,,.

,(WHO) ,30 ,WHO ,

2,4 96 %,,HSR E

,: (1) 10-3mg,,,,2 10-4mg 10-6mg,,

(2) 1 %,20 ,1 %,2 ,,3

(KH2PO4)

1.2.(INH)(RFP)(TB1)(TH1321)(SC).SM-SO4)EMB-Cl)(KM-SO4)(CPM-SO4)(VM-SO4)(PAS-Na)

3.

=/1000mg % g/ml % ml

4 5.5~10ml,-20 3 RFPTB1TH1321

100ml1ml7ml85 50min.37 24h4 1

1 12 20.Tween-80MacFaarland No.1mg/ml

2.122mg/ml0.01 mg/ml0.1 mg/ml(a):22100222mg/ml2 ml0.01 mg/ml10010-4 mg/ml220.7mm,3mm0.01ml

2. (b)100.5ml1mg/ml4.50.1mg/ml.1010-2 mg/ml10-4 mg/ml

3.:221(0.01ml) 10-2 mg/ml10-4 mg/ml10-4 mg 10-6 mg4.:37 4

1..(1)50: (2)50~100: 1+(3)100~200: 2+(4)(200~500): 3+(5)(500): 4+

2.:=/*100%1%

1.(10-4 mg/ml)202.(H37RV)10-3 mg10.1ml 1%(BaCl2)9.9 ml 1%(H2SO4)

2~3mg/ml37 4

INHEMB 10-4+++++3 10-62690%34.60.1Rs

1234H37RV

12345

4

198012%89.6%8.0%4.3%

28 28 TNM

PNB-2-TCH

PNBTCH28 + NTM + + + + + +

3~4

7H9

L-JL-J

-45

Gen ProbeGEN-PROBE HPARNADNARNADNAGEN-PROBE LEADER

Gen ProbeGen ProbeGen ProbeMTDrRNA

Gen Probe18MTD2.53.5rRNAMTD