¢0> SPECTRACELL LABORATORIES LABORATORY REPORT · 2019-12-10 · Antioxidants Glutathione Cysteine Coenzyme 0 -10 Selenium Vitamin E (A-tocopherol) Alpha Lipoic Acid Vitamin C SPECTROXTM

¢0> SPECTRACELL LABORATORIES

LABORATORY REPORT Account Number: 186506

John Doe, M.D. 1234 Any Street Suite 244 Anytown, TX 77581 -1234 USA

Name: Janet Doe Gender: Female

Accession Number: Requisition Number:

Date of Collection: Date Received: Date Reported:

Summary of Deficient Test Results

Testing determined the following functional deficiencies:

Vitamin 81 Vitamin K2

Spectrox

Glutathione lmmunidex

lmmunidex 100 100.------.

77

Selenium Vitamin E

lmmunldex vs Age

DOB: 04/1 0/1971

K88809 438507

01 /10/2012 01 /11/2012 01/20/2012

1:~~~~--•

10 ToUl.I Anlioxkllln1 Pllncllon

John F. Crawford, Ph.D. Laboratory Director

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CLIA# 4500710715

SpectraCell Laboratories, Inc. Laboratory Report

Accession Number: K88809 Janet Doe

OVERVIEW OF TEST PROCEDURE 1. A mixture of lymphocytes is isolated from the blood. 2. These cells are grown in a defined culture medium containing optimal levels of all essential.

nutrients necessary to sustain their growth in cell culture. 3. The T-lymphocytes are stimulated to grow with a mitogen (phytohemagglutinin) and growth is

measured by the incorporation of tritiated (radioactive) thymidine into the DNA of the cells .

The growth response under optimal conditions is defined as 100%, and all other growth rates are compared to this 100% level of growth.

For example - we remove vitamin 86 from the medium and stimulate the cells to grow by mitogen stimulation. Growth is measured by DNA synthesis and the rate of growth is dependent only upon the functional level of vitamin 86 available within the cells to support growth. For Vitamin 86 a growth rate of at least 55% of the growth rate observed in the optimal (100%) media is considered normal. Results less than 55% are considered to indicate a functional deficiency for Vitamin 86. Each nutrient has a different reference range that was established by assaying thousands of apparently healthy individuals.

BREAKING DOWN THE REPORT 1. TEST RESULT (o/o CONTROL) This column represents the patient's growth response in the test media measured by DNA synthesis as compared to the optimal growth observed in the 100% media.

2. FUNCTIONAL ABNORMALS An interpretation is provided for those nutrients found to be deficient.

3. REFERENCE RANGE I

This column represents how this patient's result compares to thousands of patients previously tested. A patient's result is considered deficient when it is less than the reference range.

4. GRAPHS The abnormal range of results is noted in the blue area. Abnormal results are indicated in red. The gray cross hatch area is a representation of the range of test results found In a random selection of subjects.

SPECTROX®- TOTAL ANTIOXIDANT FUNCTION SPECTROX® is a measurement of overall antioxidant function. The patient's cells are grown in the optimal media, stimulated to grow, and then increasing amounts of a free radical generating system (H202) are added. The cell 's ability to resist oxidative damage is determined. The increasing levels of peroxide will result in diminished growth rates in those patients with poor antioxidant function capacity.

INDIVIDUAL ANTIOXIDANT LEVELS In the tests for individual antioxidants, it is determined which specific antioxidants may be deficient and thus affecting the SPECTRO X® antioxidant function result. For these tests, the patient's cells are preincubated with one of the nutrient antioxidants, i.e. selenium, and then the Spectrox® test is repeated to determine if the addition of selenium improves the patient's antioxidant function. This process is repeated for each individual antioxidant.

Antioxidants tested with this process: Glutathlone, Cysteine, Coenzyme-010, Selenium, Vitamin E, and Alpha Lipoic Acid

SpectraCell Laboratories, Inc. Laboratory Test Report

1. Vitamin 81 (Thiamin)

2. Glutathione

3. Selenium

4. Vitamin E (A-tocopherol)

5. Vitamin K2

6. lmmunidex

Repletion

so mg daily

uggestions


600 mg t.i.d. (1800 mg daily) of N-Acetylcysteine (NAC). Reduce to 1200 mg daily after 3 months and retest after 6 months. Take each dose with a meal

200 mcg daliy of selenium glycinate or selenomethionine for 3 months and then reduce to 100 mcg daily

400 IU daily of mixed tocopherols

100 mcg vitamin K1 (K2 precursor) daily

lmmunidex is an evaluation of the patient's T-lymphocyte's response to mitogen stimulation. Low responses may be related to the patient's levels of stress, nutritional deficiencies, or pathology. Improvement in the patient's lmmunidex may be facilitated by correction of the functional deficiencies reported in this analysis.

Pleau note: Supplementation is usually required for four to six months to effect the repletion of a functional deficiency in lymphocytes

Suggestions for supplementation with specific micronutrients must be evaluated and approved by the attending physician. This decision should be based upon the clinical condition of the patient and the evaluation of the effects of supplementation on current treatment and medication of the patient.

SpectraCell Laboratories, Inc. Laboratory Test Report

Micronutrients B Complex Vitamins Vitamin 81 (Thiamin) Vitamin 82 (Riboflavin) Vitamin 83 (Niacinamide) Vitamin 86 (Pyridoxine) Vitamin 81 2 (Cobalamin) Folate Pantothenate Biotin

Amino Acids Serine Glutamine Asparagine

Metabolites Choline Inositol Camitine

FattvAcids Oleic Acid

Other Vitamins Vitamin D3 (Cholecalciferol) Vitamin A (Retinal) Vitamin K2

Minerals Calcium Manganese Zinc Copper Magnesium

Carbohydrate Metabolism Glucose-Insulin Interaction Fructose Sensitivity Chromium

Antioxidants Glutathione Cysteine Coenzyme 0 -10 Selenium Vitamin E (A-tocopherol) Alpha Lipoic Acid Vitamin C

SPECTROXTM Total Antioxidant Function

Patient Results % Control

76 53 86 61 21 35 9

36

33 40 55

30 68 59

76

65 81 26

48 69 44 48 55

46 52 54

32 43 97 74 79 83 50

44


Functional Abnormals

Deficient

Deficient

Deficient

Deficient Deficient

Reference Range reater than

>78% >53% >80% >54% >14% >32% >7% >34%

>30% >37% >39%

>20% >58% >46%

>65%

>50% >70% >30%

>38% >55% >37% >42% >37%

>38% >34% >40%

>42% >41% >86% >74% >84% >81% >40%

>40%

0

The reference ranges fisted in the above table are valid for ma le and female patients 12 years of age or older.

I

I I

Adequate Deficient Values In this area

Borderline represent a deficiency and may requ re nutrient repletion

Deficient or dietary changes

B Complex Vitamins

122~---...... in~---.....

Amino Acids & Metabolites 10 70 10

S7 S1 • 45 4S

n 32

20 20 Serine

Other Vitamins & Minerals !111----...... 111----...... HIO----.....,

Borderline

D Values in this area represent a borderline and may require nutrient repletion or dietary changes.

~)

40 90

JO

20

10

Cbo-ilile

72

CJmltinc

M~---.....,

Accession Number: KB8809 Janel Doe

4n~---......

Pantolhena!e

9S

~Add

111----...... 10- ---......

• • • Adequate Deficient

Values in this area Borderline represent a deficiency and

may require nutrient repletion Deficient or dietary changes

Carbohydrate Metabolism

65r------. 10.------, 10.------,

53

42 47

31

20 25 Fl\Jaosc Scnsillvl1y Olucosc-lnsulin Interaction

Individual Antioxidants 65 70 110

SS 60 100

45 90

35 80

~ 70 Glu1alhione CoonLy111C Q· lll

Borderline

D Values in this area represent a borderline and may require nutrient repletion or dietary changes.

Spectrox

100.------.

n

SS

•

10 Tow Antioxidaru Function

100

+ + ++

86 92 ----78 85

70 77

62 Sclcnlum


A Spectro• velue above 65"4-indicates a desirable stalUs for apparenlly healthy Individuals. Since an11oxldanls are pro1ectl118 nulrien!S, the most desired s1a1us would be the gr11a1asl ability to resist oxidative slress.

A Spectrox ¥81118 i.iw.n 40% I nd 85%-or•dicates an average an1ioxiaan1 lunC11on for apparenlly heahhy ind1Vlduals Art a119rage Slatus means !he ab1111y 10 resist 011datlY8 Slress similar to lhe majority of persons However. average stalus Is not Ideal, nor Is 1t clearly deficient

100

92

85

11

70 Alpha l..lfM!k: Acid Vi1runbC

lmmunidex

IOO.------,

The lmmunidex is an indication of the patient's T-Lymphoproliferative response to mitogen stimulation relative to the response of a control population. An average or weakened immune response may improve with correction of the nutritional deficiencies determined by the micronutrient testing.

50

lmmunidiu

An lmmunlde• above 65% indicates a strong response. a measuremenl of cell·medlated immune function.

An lmmunidex belwwn 40% and 115% -Indicates an average response

«) SPECTRACELL LABORATORIES

SUPPLEMENTAL INFORMATION

Name: Janet Doe Gender: Female DOB: Accession Number: K88809

Date Received: 01/11 /2012 Date Reported: 01 /20/2012 Requisition Number: ·

Account Number: Dr.Anyone 1234 Anywhere City, State USA

Laboratory Test Report SpectraCell Laboratories, Inc.

tatll :

Vitamin Bl (Thiamin)

Accession Number: Janet Doe

The patient' lymph cyt . h ve hown a deficienl talu ~ r Vitamin B 1 (Thiamin)

FunctUm:

T hia min i u ed by celJ Lo help make ene rgy from food turn . Thiamin pyropho phate i a cofactor for dehydrogenase enzyme wilh key role in cellular energy production. Thyamin pyropho phate · required for tran ketola e activity which i a component of Lhe pento e pho phate pathway, the ole ource for the ynthe i of ribo e u ed in ynlhe i of the nucleic acid (DNA and RNA). The e reaction al o produce the major ource of cellular NADPH (u ed in fatty acid bio ynthe i and other pathway ). Thiamin tripho phate is locaJized in nerve cell membrane , and play role in tran mi ion of nervou impul es and acety lcholine ynthe i .

Deficiency Symptoms:

Early thjamin deficiency lead Lo of Appetite Irritabi Li ty Fatigue

to clinical ign of: Con tipation MentaJ Depre

au ea ion PeripberaJ europathy

Clinical signs of more evere Lhiamjn deficiency ( Wemicke-Korsafoff Syndrome): Mental Confu ion Lo of Fine Motor Control Loss of Eye Coordination Weakne

Tho e at risk for thlamin defic iency include: Patients offering from Malnutrition Starvation or Malab orption Syndrome

Alcoholic Elderly Patients on re tricted diet Patient with an increa ed meta olic rate Prolonged hemodiaJy i (pregnancy lactalion, fe ver infection trauma) Ga tric partiti ning urgery Inherited Thiamin-Re pon ' ive M taboli Di order

Repletion Information:

Di tary ource rich L in B (per . erving) include: I

ul.ritionaJ upplemem Rice Bran Pork Legume bean . p a , oybean , lentil )

utritional Ye t Wheat Germ

nri hed Grain & Grain Pr duct (cereaJ )

xc ive inge tion of certai n raw fre h-water fi hand shellfi h, tea, coffee, b lucb rrie and red cabbage hould b> avoided as the e food may contain anti-th iamin factor . The 1989 RDA for Lhiamin i between 1.0-1.5 mg for adult . T here i no evid nee of thiamin toxicity form oral admini tration, e cepl for development of en iti ity in very rare case .


Statu :

Glutathione

Th patient' lymph cyt have shown a deficient statu · for GlutaLhione.

Functio11:


Glutathione i · implicated in many cellular function including antio idant protection and detoxificati n. IL i at o ential for the maintenance of cell membrane integrity in reel blood cell . Intra eltuJar glutathion concenLration are principally deri ed by intracellu lar ynthe i , as few cell directly uptake glutathione from th urr unding extracellu lar fluid. The high concentration f glutathione in virtually all cell clearly indicate it

imp rtance in metabolic and oxidative detoxification proce e . Glutathione may be con idered the preeminent antioxidant.


A wide range of human condition uch aging, cancer, athero clero i , arthriti , iral infection AID , cardiovascular, neurodegenerative d ' ease and pulmonary di eases may be produced, or made wor e by "free radical ". Their treatment or prevention often include antioxidants ach as vitamin C, vitamin E, carotenoid and elenium. Glutathione i an e ential component of the antioxidant defen e y tern: producing a " paring effect"

for both tocopherol and a corbate by reducing the oxidized form , and by eliminating hydrogen peroxide by (eacting with glutathione peroxida e. Cellular glutathione functions to decrea e the formation of oxidized LDL, implicated in the development of athero clero i . T-lymphocytes become deficient in glutathione in the progre ion of AIDS which impair immune function . Glutathione is al o required for the ynthe i of ome pro taglandins from n-3 and n-6 polyun aturated fatty acids which are important in the inflammatory r pon e. Patie nts with adult re piratory di tres yndrome are fa orably affected by treatment that increase cellular glutathione.


p rt a orbed from the ga trointe tinal lract and f rich in glutathione do n t appear to contribute to increa c in intra ellular glutathionc level . Cy teine appear to b the limiting amino acid in th intracellular ynthe i of glutathione and upplementali n with up to 2000 mg daily of -Acetyl-L-Cy tcine ppear afe. upplementation with cy teine i n t re omm nded as it ma be p orly tolerated by man patient-.

In addition, it may be rapidly o idized to L-cy tine, a I · u able form for th ·ynthc i of glutathione. n h m cy teine are generally high pr Lein food uch m at . yogun, wheat germ and egg .


Status:

elenium

The paLient' lymphocyte have h wn deficient ·tatu for clcnium.

Function:

Accession Number· Janet Doe

The trace mineral elenium function primarily as a comp ncnt of the antioxidant enzyme glUlathione pero ida e. Glutathione peroxida ea ti ity, which require elenium for activity, facili late the recycling of vitami n and , in optimizing the perf rmanc of the anti oxidant y tern. Low le el of elenium ha e been linked to a higher ri k for cancer cardiovascular di ea e, inflammatory di ea e , and other condition a· o iated with free radical damage, including aging and cataract formation. Se lenium i al o essential fo r healthy cell-mediated immun function , timulating immune propertie of lymphocyte . elenium i al o needed for the acti vati on of thyroid hormone.


Chronic low elenium intake i ociated with an incre ed ri k for heart diseas , cancer and depre ed immune function. Selenium appear Lo provide protection again t heart di ease and troke. elenium upplementation ( I 00 mcg/day) increase the ratio of HDL to LDL and inhibi platelet aggregation .

Selenium and glutathione peroxida e activity are low in patient with rheumatoid arthriti , eczema, p oria i and mo t inflammatory condition . Thi i related to the increa ed ynthesi of proinflammatory pro taglandin and leukotriene . Immune y tern function i enhanced by elenium. by contributing to higher natural killer cell (NKC) activity. Natural killer cell have the ability to de troy cancer ceU and bacterial and viral agents. Heavy metal toxicity ymptoms may be alleviated by selenium, a tin a an antagoni l. Sel nium deficiency may al o contribut to male infertility.


Selenium i safe at the level general ly u ed fo r upplem ntalion ( 100-200 mcg/day). However. taki ng more than 750 mcg of elenium per day may cau e toxicity R action uch as lo of fingernail , • kin rash and neural gical aberration . In the pre ence of iodine deficiency goiter, el nium upplem ntation ha been reported Loe acer ate low thyroid function.

lenium i a ailable in raJ different fonn . Ludie indicate Lhat inorgani ah like odium elenite ar le. effectively ab orbed and not a · biologically active a. organic forms of elenium, u ha elenomethionine or high- elenium content yea t. Rich t ource of dietary elenium are found in :

Wheal Germ Brazi l Nut Whole Wheal Bread Brown Rice

Bran Red wi Oat Turnip

The adult RDA for elenium i 50 mcg/day.

hard


Status:

Vitamin E (a-tocopherol)

The patient lymphocyte have hown a deficienl tatu for vitamin E

Function:


Vitamin E is an anLioxidant that protect cell membrane and other fat- oluble compound from oxidati ve damage by free radical . For example, the oxidative damage to LDL-chole terol appear to lead to the depo ition of cholesterol in the arterial wall leading to athero clerotic di ea e. In the pa t few year many other function of vitamin E have been clarified. Alpha-tocopherol ha direct effect on the control of inflammation, red and white blood cell production, connective ti sue growth and genetic control of cell di vi ion . Vitamin E acts to reduce free radical damage by converting arachidonic acid free radicals to le s harmful derivatives, limiting formation of pro-inflammatory cytokine . In deficiencies of vitamin E, arachidonic acid is converted to pro-inflammatory leukotrienes and cytokines. In neutralizing free radical , vitamin E i oxidized to a free radical. Conver ion back to the reduced form occur by reaction with vitamin C (a corbate).


The principle use of vitamin E is an antioxidant in the protection against heart disease, cancer, stroke and neurodegenerative disease (Alzheimer's). In addition, alpha-tocopherol supplementation is useful in treating other carctiovascular diseases, diabetes, fibrocystic breast disease, menopause ymptom and tardive dyskinesia. It may also have applications in Parkinson' Disea e and arthritis. Vitamin E is important to immune function, protecting thymic function and white blood cells from oxidative stress.

Symptoms of vitamin E deficiency include nerve damage, muscle weaknes , poor coordination, involuntary eye movements, red blood cell fragility, anemia and retrolemal fibroplasia (eye di ea e).


Vitamin E i available in many different formulation , either natural or ynthetic. atural form of vitamin E are de ignated d- a in d-a-tocopherol. Synthetic form are de ignated a di-. The biologically active form of the vitamin i the d- form and it i recommended fo r upplemenlalion over the di - ( ynthetic) form . Beta-tocopherol , gamma-tocopherol and the al pha- and delta-tocorelinol have le than 50% of the bio logical activity than d-a-tocopherol .

The RDA for vitamin E (d-a-tocopherol) i et at 15 1.U. per day. The amount of vitamin E required i dependent upon Lhe amount of polyun a tu rated fat in lhe diet. The more polyun aturated fat in the diet, the greater the ri k for oxidative damage, and the vitamin E requirement i increased. Mo t ·tudies have utilized do e between 200-400 LU. per day. Some tudie report effective u e of vitamin Eat do e up to 3000 LU. per day withouL ob erved ide effect over a two-year period.


Status:

Vitamin K

The patient' lymphocyte have hown a deficiem Latu for vitamin K2.

Function:


The pri mary fu nction of vitamin Ki to aid in the formation of clotting factor and bone protein . [t erve a a cofactor in the production of ix protein that regu late blood clotti ng, including prothrombin. In addition, it help to form o teocalcin, a protein neces ary for the mineralization of bone. Vi tamin K also aids in the formation of gluco e into glycogen for torage in the Ii ver. In addition, it promote lhe prevention and rever aJ of arterial calcification, plague progre ion and lipid peroxidation . Deficiency may increase the risk of calcification of arterial walls, particularly in indi vidual on vitamin D upplementation (Vitamin D promote calcium ab orption). Vitamin K exists in three forms: K , a natlrral form found in plant (phylloquinone); K , which i ynthesized in the inte tine (menaquinone); and K , a y

1nthetic form that must be activated in the liver (meni dione).

3


Excessive bleeding, a history of brui ing, appearance of ruptured capillaries or menorrhagia (heavy periods) are the most common clinical symptoms of overt vitamin K deficiency, although ubclinical deficiency may not affect clotting mechanisms. Due to its critical role in bone formation , long-term vitamin K deficiency may impair bone integrity and growth, eventually predisposing a per on to osteoporosis. Anticoagulants such a Coumadin and other warfarin can deplete vitamin K by blocking the activation of prothrombin. Excess vitamin K will not adversely affect clotting function for patients. However, patients on warfarin or other blood anticoagulants should not supplement with vitamin K unless specifically recommended and approved by their physician. Other cause of deficiency include celiac disease, liver disease, certain medications (i.e. aspirin, Dilantin), very high dose of vitamin A and E (over 600 IU) and gastrointestinal disorders associated with the malab orption of fats, uch a bile duct ob truction, pancreatiti or inflammatory bowel di ea e. Repletion Information:

The RDA for vi tamin Ki I microgram (mcg) per 2.2 pounds of body weight, with 80 mcg per day (maJe ) and 65 mcg per day (female ) being the offic ially recognized amount, although therapeutic do e range Crom I 00 to 500 mcg per day. No Tolerable Upper Intake Level fo r vitamin K ha been e tabli hed. The liver ecures the amount of vitamin K required for the atu ration of clotting factor . Supplementation wnth vitamin KI i recommended a it i the precur or of vitamin K2. A a re ull patient hould receive benefit of both KI and K2. Vitamin K i a fat oluble vitamin o inge tion with fat or oil ignificantly increase ab orption. Since up to 50% of the vitamin i manufactured by bacteria in the gut, the balance of inte tinaJ microflora i important in maintaining adequate endogenou production of vitamin K. Antibiotic u age can up et thi balance. Exogenou food ource particularly rich in vitamin K include kale, green tea, turnip green , pinach, and broccoli. Other ource include lettuce, cabbage, beef Li er, asparagu , watercre , chee oat , pea , and whole wheal.

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¢0> SPECTRACELL LABORATORIES LABORATORY REPORT · 2019-12-10 · Antioxidants Glutathione Cysteine Coenzyme 0 -10 Selenium Vitamin E (A-tocopherol) Alpha Lipoic Acid Vitamin C SPECTROXTM