Sleep Analysis Software

SleepSign® for Animal

Summary1. History

Industry standard software developed withOBI and accepted world wide

2. Data acquisitionAny devices can be integrated

3. Sleep stage scoringEasy enough for beginnersSophisticated enough for experts

4. Post analysisQuick and easy reporting

Osaka Bioscience Institute

JapaJapann

OsakOsakaa

http://www.obi.or.jp

•Discovered somnogenic effect of PGD2 Discovered somnogenic effect of PGD2

•Citation of published scientific papers, world NO.1 Citation of published scientific papers, world NO.1

•KISSEI co-developed SleepSignKISSEI co-developed SleepSign

International customers•13 countries, 240 licenses 13 countries, 240 licenses •Cited by most highly ranked scientific Cited by most highly ranked scientific papers papers

JapaJapann USAUSAOsaka Bioscience Osaka Bioscience

InstituteInstituteMolecular Behavioral Biology

Tokyo Medical and Dental UniversityTokyo Medical and Dental UniversityInstitute of Biomaterials and Bioengineering

Stanford UniversityStanford UniversityCenter for Narcolepsy

Eastern Virginia Medical CollegeEastern Virginia Medical CollegeDepartment of Pathology and Anatomy

Harvard Medical SchoolHarvard Medical SchoolDepartment of Neurology

Beijing universityBeijing universityDepartment of pharmacology

Korea Institute of Science Korea Institute of Science and Technologyand TechnologyCenter for Neuroscience

Waseda-Olympus InsWaseda-Olympus Institute. Singaporetitute. SingaporeCenter for Neuroscience

Chinese university Chinese university of Hong Kongof Hong KongCenter for Neuroscience

GlaxoSmithKlineGlaxoSmithKline

Eli LillyEli LillyPfizerPfizerAsiaAsia

EUEUFudan universityFudan university

Hôpital Pellegrin.Boudreaux France

Jawahar Lal Jawahar Lal Nehru UniversityNehru University

IndiaIndia

Import directly

SleepSign

Digital data import

BIOPAC

DSI Dataquest A.R.T

EDF file (European Data Format)

A/D conversion card

Analogue recording

Analogue amplifier

Analogue recording + Video

Analogue amplifier

MPEG encoder card

Camera

A/D conversion card

EEG is a waveA wave is defined by

Amplitude and Frequency

Amplitude

FrequencyFrequency

Human sleep EEG

FFT (Fast Fourier Transformation)

1 sec

FFT maps the power of each frequency bins in a wave

2Hz

Waveform Power spectra

μV2

Hz

μV2

Hz

Rat sleep stages

Wake to NREM

EEG

EMG

FFTHigh amplitude slow wave

Wake NREM NREM

EMG becomes flat

NREM to REM

EEG

EMG

FFTLow amplitude, theta wave

NREM REM REM

EMG is flat

REM to Wake

EEG

EMG

FFT

WakeREM Wake

Not rhythmical

EMG becomes active

Automatic scoring• Easy operation• Computer assists criteria decision• 85-95% agreement to human visual scoring

Quality of sleep

The number of brief wake

Duration of sleep wake episodesTotal amount of each stage

Sleep onset

Evaluate efficacies on sleep regulation

Delta power for sleep stage

Quantity of sleep

Various analysis parameters

The number of Brief wake (Hypnogram)

Timescale graph of sleep stages

4 s

Brief Wake between NREM Sleep in Mice

EEG

EMG

brief wake(<16 s)NREM NREM

WT

H1RKO

W

R

N

8:00 8:15 8:30 8:45

W

R

N

Huang, et al., PNAS 2006

Total amount of sleep stage (Stage Graph)

Total amount spent in each sleep stages per time bins

Light Dark

REMWakeREM

NonREM

Mizoguchi & Eguchi et al., PNAS 2001

Duration of sleep episodes (Vigilance State Parameters)

Huang, et al., PNAS 2006

The amount of delta power (Average FFT)

Stage dependent average FFT per time bin

NREM/Light’’’’’’’’’’’’’’’'

NREM/Dark

Multi Animal ScreeningNew function

Rat sleep stages

Simple interfaceSD

Rat

_1SD

Rat

_2S

D R

at_3

SD R

at_4

Simple pre –setting parameter menu

multi screening is executed after pre-setting common value of screening parameters

Initialize Scoring Ratio

R 8% W 12% NR 80%

Step1: calc each portions of R/W/NR

NR 80%

Step2: multiple ratio of Light/Dark phase

Light phase 80%Dark phase 20%

NR 16% NR 64%

X

R6.4% W 9.6% NR 64%R 1.6%W 2.4% NR 16%

Dark phase 20% Light phase 80%

Adjusting

WR

NR

Loco

mot

ion

activ

ity (c

ount

)

EE

G

pow

er(

V2 )

EE

G/

(+

) ra

tio(%

)

EM

Gin

tegr

al(

V/s

ec)

12:00 14:00 16:00 24:00 02:00 04:00Clock time

Light phase Dark phaserat

Locmoter

Summary1. History

Industry standard software developed withOBI and accepted world wide

2. Data acquisitionAny devices can be integrated

3. Sleep stage scoringEasy enough for beginnersSophisticated enough for experts

4. Post analysisQuick and easy reporting

AcknowledgmentsDr. T. Mochizuki (Beth Israel Deaconess Medical Center and Harvard Medical School)

Dr. X. Tang (Eastern Virginia Medical School)

Dr. Z. Huang (Fudan University and Osaka Bioscience Institute)

Dr. O. Hayaishi, Dr. Y. Urade (Osaka Bioscience Institute)

Dr. S. Nishino, N. Fujiki (Stanford University, Center for Narcolepsy)

Dr. Z. Hu (Third Military Medical University)

Dr. S. Inoue, K. Honda (Tokyo Medical and Dental University)

Dr. N. Eguchi (Waseda-Olympus Bioscience Reserach Institute)