01b_AKTA

KTAexplorer

amersham bioscience

amersham bioscience

amersham bio

science

amersham b

ioscience

KTA system Training GuideExplorer 100 / 10

KTAexplorer

amersham bioscience

amersham bioscience

amersham bio

science

amersham b

ioscienceBox

MonitorConductivitypH

UV Detector3 Wavelengths

Pumps

ColumnValves

SampleValve

Buffers

pH/cell

electrode

Sampleholder

Pump A

Buffer switchingvalve

OutletValve

KTAexplorerReverse Flow Valve (inside)Std on KTAexplorer 100 only Injector

amersham b

ioscience

amersham bioscience

amersham bioscience

amersham bio

science

KTA system Training GuidePurifier 100 / 10

23 4 5

6781

4

A

Systempumps

Mixer

Conductivitymonitor

pH detector

In-linefilter

Samplepump

Injectionvalve (V1)

Reverseflow valve (V7)

Outletvalve (V4)

Sample selectionvalve (V5)

Columnselectionvalve (V2)


3 Wavelengthdetector

23 4 5

6781

23 4 5

6781

23 4 5

6781

23 4 5

6781

24

7

3

165

24

7

3

165

UV

Cond

pH

KTAexplorer 100

B

4

A

Systempumps

Mixer

FractioncollectorFrac-950

Conductivitymonitor

pH detector

In-linefilter

Samplepump

Injectionvalve (V1)

Reverseflow valve (V7)

Outletvalve (V4)





23 4 5

6781

23 4 5

6781

23 4 5

6781

23 4 5

6781

24

7

3

165

24

7

3

165

UV

Cond

pH

Buffer selectvalve (V6) Air

sensor23 4 5

6781

Buffer selectvalve (V6)


B

B4

A

Systempumps

Mixer

Conductivitymonitor

pH detector

In-linefilter

Injectionvalve (V1)




23 4 5

6781

23 4 5

6781

UV

Cond

KTApurifier 10

23 4 5

6781

B

4

A

Systempumps

Mixer


In-linefilter

Samplepump

Buffer selectvalve (V6)

Injectionvalve (V1)

Outletvalve (V4)




23 4 5

6781

23 4 5

6781

23 4 5

6781

23 4 5

6781

KTApurifier 10 S

24

7

3

165

24

7

3

165

Buffer selectvalve (V6) 23 4 5

6781

23

45

67

81


Outletvalve (V4)

PUMP P-900 Inlets

Buffer Select Valve IV-908

Pump A Pump B

Mixer

12

3 4 56

78

A11A12 A13

A14A15

A16A17

A18

BA

PUMP P-900 Inlets

Water Salt

Buffer Select Valve IV-908

Pump A Pump B

Mixer

A2 A1 B1 B2

SwitchingValves

12

3 4 56

78

A11A12 A13

A14A15

A16A17

A18

PUMP P-900 Manually Priming Buffer Inlet Lines

Open

Draw Air out ofBuffer Line

Manual Flowpath

A1 or A2 BufferValveA

Manual Flowpath

B1 or B2 BufferValveB

Pump A Pump B

Common inlet A2 A1 B2 B1Common inlet

PUMP P-900 Back - Wash Lines

20% Ethanol : Water

Pump Back-WashLines

Wash OUT

WashIN

BufferIN

BufferOUT

High pressurePiston seal

Ball outletcheck valve

Ball inletcheck valve

Piston

Backwash pump diaphragm

IN-LINE one-wayvalve

Syringe fitting

LOAD (sample) INJECT (sample) LOAD (wash loop)

1

7

6

5

2

3

4

Pump

Column

Waste

PV-9081

2

34 5

6

7

8

Sample

1

7

6

5

2

3

4

Pump

Column

Waste

PV-9081

2

34 5

6

7

8

Sample

1

7

6

5

2

3

4

Pump

Column

Waste

PV-9081

2

34 5

6

7

8

Sample

ON-LINE FILTERS

Polymer Support

P-903 ( 10 ml/min Pump )Low Flow HPLC Filter

P-901 ( 100 ml/min Pump )High Flow Fibre Filter

17

65

2

3

4

PUMPLOADSAMPLE

Waste

COLUMN

Waste

LOAD

1

7

6

5

23

4

PUMP

Waste

COLUMN

Waste

INJECT

17

65

2

3

4

PUMP

Waste

COLUMN

Waste

WASTE

INJECTION VALVE INV- 907

SAMPLE LOADING METHOD

1

7

65

2

3

4

PUMPLOADSAMPLE

Waste

COLUMN

Waste Superloop

Luer LockAdapter

1

7

6

5

2

3

4

PUMPLOADSAMPLE

Waste

COLUMN

Waste

Needle Port

Always tighten by hand with needle inserted

Do Not Overtighten

Volume Max. allowed column pressure

Code No.

10 ml 4 Mpa 18-1113-81 50 ml 4 Mpa 18-1113-82 150 ml 2 Mpa 18-1023-85

SUPERLOOPS

10 ml

150 ml50 ml

BUFFERFLUSH

SAMPLELOAD

SAMPLEINJECT

SAMPLEFLUSH

WASTE PUMP orSYRINGE COLUMN COLUMN

Plunger

Buffer

Sample

EtchedGroove

COLUMN SELECTION VALVES (PV-908 )

Pump

MONITOR

REVERSE FLOW VALVE ( INV-907 )

Column

Pump

Downward FlowPosition 1

Extra Tube

MONITOR

StopPlug

17

65

2

34

1

Column Extra Tube

MONITOR

StopPlug

2

Upward FlowPosition 2

Pump7

65

34

COMPRESSION FITTINGS

Untightened

Correctly tightened

Over tightened Tubing Restricted

Obstructed

PEEKTM Tubing

Gray, 1/16x 1.0 mm ( 7.85ul / cm )

Green, 1/16x 0.75 mm ( 4.42ul / cm )

Orange, 1/16x 0.5 mm ( 1.96ul / cm )

Blue, 1/16x 0.25 mm ( 0.49ul / cm )

Teflon / Tefzel Tubing

3/16 OD

1/16 OD

18-1112-47

1/8 OD 18-1121-16

18-1119-74

BA

0%

B

A

20%

COLUMN TOP

CommandEffect8 mls

System Compensation Volume

Equilibration Phase

0%B

20%B

8mls

COLUMN TOP

BA

0%

COLUMN TOP

B

A

100%

COLUMN TOP

Command 100%

Effect 16 mls

Gradient Delay Volume

100%B

Gradient Delay

Gradient Phase

Col

umn

Pres

sure

( co

mpe

nsat

ion

) Flo

w R

estr

icto

r

Col

umn

Har

dwar

e Pr

essu

re L

imits

Col

umn

Mat

rix

Pres

sure

Lim

itsC

olum

n Pr

essu

res

Col

umn

Shat

ters

DA

NG

ER

OU

S

Mat

rix

Col

laps

es E

XPE

NSI

VE

GEL

Pre

ss D

rop

0.3

MPa

(0.3

-0.

0)

GEL

Pre

ss D

rop

0.3

MPa

(0.3

-0.

0)

0.3

Rea

ding

0.3

Rea

ding

GEL

Pre

ss D

rop

0.1

MPa

(0.3

-0.

2)

GEL

Pre

ss D

rop

0.1

MPa

(0.3

-0.

2)

Pum

p0.

3 M

paPu

mp

0.3

Mpa

Atm

osPr

ess

0.0

MPa

Atm

osPr

ess

0.0

MPa

0.0

MPa

0.0

MPa

Col

umn

Pres

s D

rop

0.3

MPa

(0.3

+ 0

.0)

Col

umn

Pres

s D

rop

0.3

MPa

(0.3

+ 0

.0)

Col

umn

Pres

s D

rop

0.5

MPa

(0.3

+ 0

.2)

Col

umn

Pres

s D

rop

0.5

MPa

(0.3

+ 0

.2)

0.5

Rea

ding

0.5

Rea

ding

0.2

MPa

Flow

Res

tric

tor

0.2

MPa

Flow

Res

tric

tor

Pum

p0.

3 M

paPu

mp

0.3

Mpa

0.2

MP

a0.

0 M

Pa

Gel

Bot

tom

Pre

ssur

e

0.3

MP

a0.

3 M

Pa

Gel

Top

Pre

ssur

e

Pre-

colu

mn

pres

sure

= 0

MPa

Pre-

colu

mn

pres

sure

= 0

MPa

Documents

01b_AKTA